1.Status quo and prospect of clinical molecular imaging.
Acta Academiae Medicinae Sinicae 2009;31(2):121-123
Medical molecular imaging not only promotes the development of medical imaging, but also pushes research progress of life science and benefits the amalgamation of multi-subjects in medical imaging. This editorial overviews the history and development trends of medical molecular imaging.
Humans
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Molecular Imaging
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trends
2.Whole body diffusion weighted imaging: a new era of oncological radiology.
Zheng-Yu JIN ; Hua-Dan XUE ; Hua TAO
Chinese Medical Sciences Journal 2008;23(3):129-132
Cancer has become the leading cause of mortality in the urban area of China. Whole body diffusion weighted imaging (WB-DWI), also known as virtual positron emission tomography, has gradually become accepted as an image tool in tumor localization, characterization, staging and monitoring response to therapy or tumor recurrence. Our article aimed to summarize the limited initial clinical use of WB-DWI in the referred area, and to analyze the most potential advantage of WB-DWI in therapeutic monitoring and tumor staging. WB-DWI as a highly sensitive, completely non-invasive, well-tolerated and low price technique has a promising furture in tumor assessment. Profound clinical study is necessary for its further application improvement.
China
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Diffusion Magnetic Resonance Imaging
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methods
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Humans
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Neoplasm Staging
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Neoplasms
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diagnosis
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pathology
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Whole Body Imaging
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methods
3.Application of right heart contrast echocardiography in patients with transient ischemic attack of unknown cause
Xinyu WANG ; Hong JIN ; Lei WANG ; Dan YU
Chinese Journal of Medical Imaging Technology 2017;33(4):487-489
Objective To explore the application value of right heart contrast echocardiography in patients with transient ischemic stroke (TIA) of unknow cause.Methods Totally 120 patients with TIA (TIA group) and 60 cases of normal healthy volunteers (normal group) were enrolled.Two groups underwent right heart contrast echocardiography to observe whether there was a foramen ovale arteriosus (PFO) or not.Diagnostic criteria were left ventricular internal appearing micro bubble≥5 that came from the right atrium to left atrium in three cardiac cycles.Results Small and medium amount of right to left shunt had no significant difference between TIA group and normal group (both P>0.05),and large amount of right to left shunt had significant difference between the two groups (P<0.01).Conclusion Contrast echocardiography can effectively diagnose of PFO,and amount of right to left shunt of PFO has relationship with the onset of TIA.
5.Analysis of gastric bilirubin absorbance values and gastric pH monitoring in children with primary duodenogastric reflux.
Mi-Zu JIANG ; Xiao-Lei HUANG ; Jin-Dan YU
Chinese Journal of Pediatrics 2007;45(4):301-303
Adolescent
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Bilirubin
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metabolism
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Child
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Child, Preschool
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Duodenogastric Reflux
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metabolism
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Esophageal pH Monitoring
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Female
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Humans
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Male
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Stomach
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physiopathology
6.A review of detection methods for human bocaviruses.
Yan LU ; Dan-Di LI ; Yu JIN ; Zhao-Jun DUAN
Chinese Journal of Virology 2014;30(3):298-302
Human bocavirus (HBoV) 1-4 have been detected both in respiratory and stool samples since the first HBoV was discovered in 2005. HBoV-1 is mostly associated with respiratory infection, while HBoV 2-4 are usually associated with intestinal tract infection. A variety of signs and symptoms have been described in patients with HBoV infection, including cough, wheezing, pneumonia, and diarrhea, but the research on pathogenic mechanism of HBoV is limited because HBoV cannot be cultured in vitro due to the lack of appropriate host cells. Three-dimensional epithelial cell culture, reverse genetics, and viral metagenomics are identified as novel tools that may promote the research on pathogenic mechanism of HBoV and the discovery of new viruses. This review summaries currently available diagnostic approaches such as electron microscopy, cell culture, PCR, and immunoassay in order to provide a method reference for indepth research on HBoV.
Animals
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Human bocavirus
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genetics
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growth & development
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isolation & purification
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pathogenicity
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Humans
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Parvoviridae Infections
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diagnosis
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virology
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Viral Proteins
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genetics
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metabolism
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Virology
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methods
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Virulence
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Virus Cultivation
7.Laparoscopic small bowel resection in the treatment of small intestinal tumors.
Lei CHEN ; Xiao-dan JIN ; Mai-yu XUN
Chinese Journal of Oncology 2010;32(10):799-780
Adenocarcinoma
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surgery
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Adenoma
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surgery
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Adult
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Aged
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Female
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Follow-Up Studies
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Gastrointestinal Stromal Tumors
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surgery
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Hemangioma
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surgery
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Humans
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Ileal Neoplasms
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surgery
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Jejunal Neoplasms
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surgery
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Laparoscopy
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methods
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Lymphoma
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surgery
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Male
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Middle Aged
8.Microbial community in the Anammox process of thermal denitration tail liquid.
Jin LI ; Deshuang YU ; Dan ZHAO ; Xiaochen WANG
Chinese Journal of Biotechnology 2014;30(12):1865-1875
An anaerobic sequencing batch reactor (ASBR) was used to treat thermal denitration tail liquid and microbial community was studied. Activated sludge was taken from the reactor for scanning electron microscope analysis. The images showed that the dominant cells in the flora were oval cocci. Its diameter was about 0.7 μm. Through a series of molecular biology methods such as extracting total DNA from the sludge, PCR amplification, positive clone authentication and sequencing, we obtained the 16S rDNA sequences of the flora. Phylogenetic tree and clone library were established. The universal bacteria primers of 27F-1492R PCR amplification system obtained 85 clones and could be divided into 21 OTUS. The proportions were as follows: Proteobacteria 61.18%; Acidobacteria 17.65%; Chlorobi 8.24%; Chlorofexi 5.88%; Gemmatimonadetes 3.53%; Nitrospirae 2.35% and Planctomycetes 1.18%. The specific anammox bacterial primers of pla46rc-630r and AMX368-AMX820 PCR amplification system obtained 45 clones. They were divided into 3 OTUS. Candidatus brocadia sp. occupied 95.6% and unknown strains occupied 4.4%.
Ammonia
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chemistry
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Bacteria
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Phylogeny
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Polymerase Chain Reaction
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RNA, Ribosomal, 16S
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Sewage
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microbiology
9.Antitumor activity of recombinant antimicrobial peptide penaeidin-2 against kidney cancer cells.
Ming-Xiang, MENG ; Jian-Fang, NING ; Jing-You, YU ; Dan-Dan, CHEN ; Xiao-Lin, MENG ; Jin-Ping, XU ; Jie, ZHANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(4):529-34
Penaeidin-2 (Pen-2) is an important antimicrobial peptide derived from the Pacific white shrimp, Penaeus vannamei, and possesses both antibacterial and antifungal activities. Recent studies suggest that recombinant penaeidins show similar activities to the native Pen-2 protein. Previous researches have shown that some antimicrobial peptides (AMPs) exhibit cytotoxic activity against cancer cells. To date, there have been no studies on the antitumor effects of Pen-2. This study evaluated the potential of recombinant pen-2 (rPen-2) in the selective killing of kidney cancer cell lines ACHN and A498, and its action mechanism. MTT assays found the maximal growth inhibition of HK-2, ACHN and A498 cells treated with 100 μg/mL rPen-2 at 48 h was 13.2%, 62.4%, and 70.4%, respectively. DNA-specific fluorescent dye staining showed a high percentage of apoptosis on cancer cells. Flow cytometry revealed that the apoptosis rate of HK-2, ACHN and A498 cells was 15.2%, 55.2%, and 61.5% at 48 h respectively, suggesting that rPen-2 induced higher apoptosis rate in cancer cells than in HK-2 cells. Laser confocal scanning microscopy demonstrated that the plasma membrane was the key site where rPen-2 interacted with and destroyed tumor cells. Scanning electron microscopy showed the morphologic changes of the cell membranes of kidney cancer cells treated with rPen-2. These results suggest that rPen-2 is a novel potential therapeutic agent that may be useful in treating kidney cancers.
10.Double-chamber stirred bioreactor improves the repaired effect of beta-tricalcium phosphate on goat knee cartilage defects
Jianwei LI ; Hui ZHAO ; Xiaoqiang ZHANG ; Lei WANG ; Liheng XIA ; Dan JIN ; Gang WANG ; Bin YU
Chinese Journal of Tissue Engineering Research 2014;(3):377-382
BACKGROUND:A preliminary experiment developed a double-chamber stirred bioreactor which can carry out osteogenic and cartilage induction at the same time.
OBJECTIVE:To explore the effects of double-chamber stirred bioreactor on the repair of goat knee cartilage defects with tissue-engineered cartilage.
METHODS:Twelve goats were selected to make bilateral femoral condyle osteochondral defects models and randomized to three groups:experimental group, implanted with the composites ofβ-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor under mechanical stimulation;control group, implanted with the composites ofβ-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor;blank control group, without treatment. After 12 and 24 weeks of implantation, general observation, Masson staining, II col agen immunohistochemical staining and histological scoring were performed.
RESULTS AND CONCLUSION:In the experimental and control groups, new cartilage tissue and bone tissue were visible, but the experimental group showed better repair effects than the control group (P<0.05). The blank control group had no cartilage formation. These findings indicate that under the mechanical stimulation by the double-chamber stirred bioreactor in vitro, the repair effect of tissue-engineered osteochondral complex on knee joint cartilage defects can be improved.