Objective To explore the relationship of vascular endothelial growth factor(VEGF),IFN-? and IL-4 and effect of Bude-sonide on their in asthmatic rats.Methods Thirty-six male SD rats were randomly divided into 3 groups:asthma group,Budesonide-treatment group and control group.On the first day of the experiment and the 8th day,the rat models of the asthma group and Budesonid treatment group were allergized by the OVA/Al(OH)3 through intraperitoneal injection,respectively.And starting from the 15th day,they were challenged by the OVA through atomization for 2 weeks.Control group was allergized and challenged by NS atomization.Budesonid treatment group was interfered in Budesonide inhalation before suscitation in 0.5 h.After 12 h the same inhal done was again in Budesonide group.Twenty-four hours after the last challenge,the rats in 3 groups were sacrificed,and blood and bronchoalveolar lavage fluid(BALF) were collected.The concentrations of IL-4,IFN-? and VEGF in serum and BALF were measured by enzyme-linked immunosorbent assay.Results The concentrations of IL-4 in serum and BALF in asthma group and Budesonide treatment group were significantly increased than those in control group(P

Objective To explore the effects of physiological deep-sea water(PDSW) on hyperthermal tolerance of Kunming (KM ) mice in the 45 .0 ℃ environment .Methods Deep-sea water from the south Chinese sea was processed ,and the metallic ele-ments dissolved in the DSW were analysed .The mice were randomly divided into 2 groups :the control group received tap water ;the experimental group treated with PDSW for 15 d .And then the mice were fed in the 45 .0 ℃ conditions .The survival time and histo-morphometric analyses of the brain ,lung ,heart ,liver and kidney were investigated .Results The survival time in PDSW-fed group was significantly longer than that of the control group (P< 0 .05) .Moreover ,histomorphometric analyses showed that PDSW could protect the brain ,lung ,heart ,liver and kidney of KM mice from the 45 .0 ℃ conditions .The results of western blot revealed that ex-pression of HSP72 of liver tissues for PDSW-fed group substantially increased ,when compared with the control mice(P< 0 .05) . Conclusion PDSW could improve hyperthermal tolerance of KM mice ,which maybe in the relation with expression of HSP72 pro-moted by PDSW .

OBJECTIVETo investigate the clinical effect of penile augmentation with superficial fascia retrocession.

METHODSThe first annular incision line was designed, reserving the inner plate 0.5 -0.8 cm. The second annular incision line was determined, according to the length of penile erection. The skin tissue between the double loops was stripped, reserving the superficial fascia and the superficial dorsal veins. Before the closure of two annual incision with no tension, the superficial fascia tissue was pushed proximally and fixed sectionally to augment penis.

RESULTSFrom Jun 2009 to Jun 2011, 60 patients with redundant prepuce or phimosis were treated with this method. During 3 to 12 months follow-up, the postoperative penile perimeter increased significantly. The glans were exposed and the prepuce looked smooth. The function of the penis maintained undamaged.

CONCLUSIONSThe penile augmentation with superficial fascia retrocession can make full use of autologous tissue to augment penis, meanwhile, redundant prepuce can be resected. It is very practical for clinical application.

Adolescent ; Adult ; Circumcision, Male ; methods ; Humans ; Male ; Middle Aged ; Penis ; surgery ; Subcutaneous Tissue ; surgery ; Transplantation, Autologous ; Young Adult

Objective To explore the effect of calcium dobesilate on the apoptosis of retinal cells in diabetic rats .Methods Thirty-six Wistar rats were randomly into three groups:normal group ( n =12 ) , model group(n=12), test group(n=12).The rat in model and test groups of diabetes was given with high fat diet for 8 weeks, and then was made by intraperitoneal injection of streptozotocin ( STZ ) .After successfully es-tablished the diatetes animal model , the rats in test group was lavaged with 150 mg? kg -1? d-1 calcium dobesilate continuous 8 weeks, the rats in normal and model group were given same amount of normal saline . Neuronal apoptosis in retina was detected by TdT -mediated dUTP nick end labeling ( TUNEL ) . The expression of B -cell lymphoma -2 ( Bcl-2 ) , Bcl -2 assaciated X protein ( Bax ) and p53 protein and mRNA were assayed by Western blot and reverse transcription -polymer-ase chain reaction.The activity of cysteinyl aspartate specific proteinase 3 ( Caspase 3) was measured by spectrophotometry .Results Compared with normal group , apoptotic index of retina , the expression of Bax , p53 protein and mRNA , the activity of Caspase 3 was increased ( P<0.01 ) , the expression of Bcl -2 protein and mRNA was decreased in model group ( P<0.01 ) . The expression of Bax , Bcl -2 and p53 mRNA was ( 0.20 ±0.03 ) vs ( 1.00 ±0.09 ) , ( 1.39 ±0.10 ) vs (0.21 ±0.02);(0.28 ±0.05) vs (0.88 ±0.08), in normal group and model group, respectively.Compared with model group,apoptotic index of retina (12.40 ±1.24), the expression of Bax (0.57 ±0.05) and p53 (0.35 ±0.03) protein, the expression of Bax ( 0.56 ±0.05 ) and p53 ( 0.36 ±0.04 ) mRNA, the activity of Caspase 3 (1.72 ±0.17) in test group were all decreased.While the expression of Bcl -2 protein (0.63 ±0.06) and mRNA (0.74 ±0.04 ) were increased in test group ( all P<0.01 ) .Conclusion The calcium dobesilate inhibit neuronal apoptosis by regulation expression of cell apoptotic related protein .

Objective To investigate the distribution and antimicrobial resistance of clinical isolates of Acinetobacter baumannii isolated from our hospitalized patients from 2008 to 2013 , and analyze the correlation be-tween the medication frequency and the drug resistance .Methods The distribution and the drug susceptibility testing for Acinetobacter baumannii isolated from clinical specimens were retrospectively analyzed .The using trend of antibiotics was analyzed by calculation the DDDs .The relation-ship between the drug resistance rate and defined daily dose ( DDDs) was statistically analyzed.Results Acinetobacter baumannii varied in drug resistance,the drug resistance rates to penicillins ,cephalosporins,quino-lones,and carbapenems were high.In 2013,no antibacterial drugs is sen-sitive to Acinetobacter baumannii rate of more than 50%.The resistance rate of Acinetobacter baumannii was negatively correlated with the dosage of piperacillin/tazobactam.Conclusion The resistance rate of Acineto-bacter baumannii is high.And the isolates are multidrug resistant .

Objective To investigate the impact of adding folic acid,vitamin B12 and probucol to standard antihypertensive medication on plasma homocysteine (Hcy) and asymmetric dimethylarginine (ADMA),serum NO and eNOS of essential hypertensive patients.Method A total of 120 patients with hypertension were randomly divided to three groups (n =40 each):group 1 (standard medication),group 2 (adding folic acid 5 mg/day and vitamin B12 500 μg twice daily) and group 3 (adding folic acid 5 mg/day and vitamin B12 500 μg twice daily and probucol 500 mg twice daily).Plasma Hcy and ADMA,serum NO and eNOS levels were observed at baseline,2 and 12 weeks after various therapy.Results In group 1,concentrations of plasma Hcy [(23.06 ± 14.15) μmol/L,(23.67 ± 12.31) μmol/L,(23.25 ± 11.64)μmol/L],ADMA[(0.21 ± 0.12) μmol/L,(0.23 ± 0.13) μmol/L,(0.21 ± 0.09) μmol/L] and serum NO [(64.14 ± 15.07) μmol/L,(65.29 ± 15.04) μmol/L,(65.32 ± 13.58) μmol/L],eNOS[(20.02 ±4.50) μg/L,(20.79 ± 4.03) μg/L,(19.82 ± 5.70) μg/L] remained unchanged during the 12 weeks therapy (all P ＞0.05).In group 2,concentrations of plasma Hcy [(12.54 ±6.49) μmol/L] and ADMA [(0.18 ± 0.07)μmol/L] were significantly decresed after the treatment of 12 weeks than the treatment baseline value [(21.51 ± 7.82) μmol/L,(0.20 ± 0.12) μmol/L] and 2 weeks value[(19.38 ± 8.14)μmol/L,(0.21 ± 0.12) μmol/L],however the concentrations of serum NO and eNOS showed contrary results of the Hcy and ADMA's.(all P ＜0.05).In group 3,similar changes occurred at 2 weeks after therapy (P ＜0.05 2 weeks vs.baseline and 12 weeks vs.2 weeks).Plasma ADMA level was positively correlated with Hcy at baseline (r =0.546,P ＜ 0.05).Conclusions Supplementation of folic acid,VitB12 and/or probucol helps to improve endothelial function and reduce plasma Hcy and ADMA levels in patients with hypertension.

OBJECTIVETo explore an effective method for treatment of senile habitual constipation.

METHODSSixty-four cases were randomly divided into a treatment group treated with moving cupping at Hechelu plus Shenque (CV 8) Bazhenxue moxibustion, and a control group with oral administration of Tab phenolphthaleinum, 32 cases in each group. Improvement of constipation in the patients were observed and compared.

RESULTSThe short-term (14 days) clinical cured-markedly effective rate was 84.4% in the treatment group, which was significantly better than 59.4% of the control group (P < 0.05), and there was a significant difference between the two groups in the long-term (2 months) therapeutic effect (P < 0. 01).

CONCLUSIONMoving cupping at the Hechelu combined with Shenque (CV 8) Bazhenxue moxibustion is an effective method for senile habitual constipation.

Acupuncture Points ; Administration, Oral ; Constipation ; Humans ; Moxibustion

OBJECTIVETo explore an effective method for treatment of tennis elbow.

METHODSOne hundred and twenty-eight cases were randomly divided into a treatment group and a control group, 64 cases in each group. The treatment group were treated by electroacupuncture at Hegu (LI 4) and Taichong (LR 3), combined with cake-seperated moxibustion at Ashi points and Shousanli (LI 10) in the affected elbow, and the control group by routine block treatment of 1 mL lidocane and 25 mg prednisone.

RESULTSThe cured rate and the effective rate were 40.6% and 93.7% in the treatment group, and 25.0% and 78.1% in the control group, respectively, with a significant difference between the two groups (P < 0.05).

CONCLUSIONElectroacupuncture combined with cake-separated moxibustion is an effective method for tennis elbow.

Acupuncture Points ; Elbow Joint ; Electroacupuncture ; Humans ; Moxibustion ; Tennis Elbow

OBJECTIVETo establish a novel Myc gene transgenic mouse model for spontaneously forming B-lymphoma and assessing its tumorigenesis potential.

METHODSFreshly isolated hematopoietic progenitor cells served as the target for Myc gene transfer mediated by a retrovirus vector. These cells were engrafted into C57BL/6 mice with (60)Co-gamma ray radiation in advance. Tumor latency was measured and the tumor loaded mice were followed for survival time. Tumor was identified with histology and immunostaining. The exogenous Myc gene was detected by Western blot (in liver, spleen, tumor tissue) and flow cytometry (FCM) \[in bone marrow (BM)\].

RESULTSMice BM-infected with mutant Myc gene more readily gave rise to B-cell lymphomas than those infected with wild type Myc gene did Myc gene was expressed highly in BM and tumor tissues but not in liver and spleen.

CONCLUSIONOur model will be a tool in assessing the transforming potential of Myc mutants and in studying cooperation between Myc and other oncogenes. Mutant Myc is more effective than wild-type Myc in promoting B cell lymphomagenesis in mice.

Animals ; B-Lymphocytes ; Cell Transformation, Neoplastic ; Flow Cytometry ; Lymphoma ; Lymphoma, B-Cell ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Retroviridae Infections

OBJECTIVETo express the cloned gene glycoprotein I (gpI) of varicella-zoster virus (VZV), Beijing VZV 84-7 strain in insect cells and to purify its expression product.

METHODSThe gene coding for gpI of VZV was amplified from viral DNA by PCR and cloned into baculovirus transfer vector (pBacPAK9), and recombinant transfer vector plasmid pBacVZVgpI was obtained. The inserted gpI gene in the pBacVZVgpI was sequenced. Insect cells Sf 9 were co-transfected with the recombinant transfer vector plasmid pBacVZVgpI and wild type linear baculovirus BacPAK6 (digested with Bsu36I) DNA. The recombinant baculoviruses containing the VZV 84-7 gpI gene was isolated through several rounds of limited dilution. Recombinant protein gpI was expressed in insect cells Sf 9, postinfected with recombinant baculoviruses. The expressed recombinant gpI was purified by lectin affinity chromatography and its antigenicity and immunogenicity were investigated.

RESULTSThe gene coding for gpI of VZV was obtained by PCR and the gpI gene of pBacPAK9 was confirmed by DNA sequencing. The recombinant gpI was expressed in insect cells Sf 9, post-infected with recombinant baculovirus and identified by SDS-PAGE and western blotting, with its product in cell culture reaching the peak in 72 hours and with a molecular mass of 58 kd and 70 kd, the same as theoretical values. Results of immunoassay with cell lysates infected by recombinant baculoviruses indicated that recombinant protein expressed in insect cells had ability of eliciting specific antibodies against native VZV in mice and complement-dependent neutralizing antibodies. The purified recombinant gpI gave a product with a purity of more than 80%. ELISA and Western-blot analysis demonstrated that purified protein had specific VZV antibody-binding activity. This suggested that the recombinant gpI expressed in insect cells had the same biological characteristics as its native counterpart.

CONCLUSIONBaculovirus-insect cells could be used to express the gene of VZV gpI, which could provide a basis for quantitative analysis of VZV antigen, and preparation of its subunit vaccine.

Animals ; Cell Line ; DNA, Viral ; genetics ; Enzyme-Linked Immunosorbent Assay ; Gene Expression ; genetics ; Genetic Vectors ; genetics ; Polymerase Chain Reaction ; Recombinant Proteins ; genetics ; isolation & purification ; Spodoptera ; cytology ; genetics ; Viral Envelope Proteins ; genetics ; isolation & purification