It is discussed in this paper the origins and pathogenesis of treating senile disease from spleen in TCM,and the relationship between various methods of invigorating the spleen and senile deficiency syndromes.It has been proposed that the therapy principles of invigorating qi and spleen,stimulating the appetite to awake the splee,primering blood to go back to the spleen,dehumidification to activate spleen-energy,warming the kidney and spleen,dispersing the depressed liver-energy to belifit the spleen,going on dieting to belifit the spleen and clearing away heart-fire to support spleen etc.are used to prevent and cure senile diseases.It also listed the corresponding treatment prescriptions for the elderly patients.It provides a theoretical basis for treatment of senile diseases from the governance of spleen.And it provided a realistic basis and the implementation of the method for the prevention and treatment of elderly patient's deficiency aging.

0.05).The AI of tumor cells was(9.7?0.9)% in mice treated with endostatin compared to that (1.9?0.4)% in the group of control ( P

Objective To evaluate the quality of immunological diagnosis kits of schistosomiasis. Methods The national reference IgG serum of schistosomiasis was applied for evaluating eight immunological diagnosis kits of schistosomiasis. Results All eight diagnosis kits had no National Production Certificate issued by State Food and Drug Administration. Six kits had good quality with high precision. After the physical examination, two kits did not meet the quality standards. Conclusions The good and bad quality of immunological diagnosis kits of schistosomiasis used in market is intermingled. The quality of these kits should be monitored.

Objective To evaluate the clinical effect of traditional Chinese medicine fumigation and herbal plaster for the patients with anal abscess. Methods A total of 140 patients with anal abscess were randomly divided into 2 groups, 70 in each group. The control group were treated with different operation methods according to the patient's health condition. the Potassium Permanganate solution (1:5 000) bath was used after surgery in the conventional anti anti-infection treatment; and the observation group were treated with herbal fumigation and plaster on the basis of the control group. The wound was treated in the both groups in the following 3 months after operation. The wound swelling disappearance, carrion shedding and healing time, pain relief, medical records of infection and recurrence were measured after the treatment and follow-up period. Results The total effective rate of the observation group was 100% (70/70), and the control group was 91.4% (64/70). The difference between the 2 groups was statistically significant (χ2=4.322,P=0.037). After 3, 7 days, the pain relief in the observation group was better than that in the control group (Z=9.742, 16.860,P=0.008, 0.001). After treatment, the observation group swelling (4.9 ± 0.7 dvs. 6.1 ± 0.7 d,t=10.142), carrion shedding time (3.8 ± 0.5 dvs. 4.2 ± 0.4 d,t=5.227), the wound healing time (22.1 ± 4.0 dvs. 26.9 ± 4.0 d,t=7.099) were lower than those in the control group (P<0.01). During follow-up, 4 cases (5.7%) with secondary infection and 2 cases (2.9%) with recurrence were in the observation group, but 20 cases (28.6%) and 6 cases (8.6%) in the control group.There were significantly difference of the secondary infection and recurrence between two groups (χ2= 6.092,8.934,P= 0.034, 0.001). 2 groups of secondary infection, the differences were statistically significant. Conclusions The combination of traditional Chinese medicine fumigation and herbal plaster could improve the wound healing time, reduce the secondary infection and recurrence rates of patients with anal abscess.

Objective To detect the expression of microRNA(miRNAs)in the late stage terminal hindgut de-velopment in fetal rats.Methods Twenty -four female rats were randomly divided into 2 groups.They were matched in proportion of male and female 21 .The experimental rats(n =1 2)received 1 0 g/L ethylenethiourea (1 25 mg/kg)by gavage on gestational day 1 1 ,and the control rats (n =1 2)received same amount of distilled water.The fetal rats were obtained by caesarean section on the gestational day 1 6 in each group.One centimeter rectum was taken out from 2 similar weight fetal rats for extracting total RNA by Trizol method.There were 24 fetal rats in each group.Chip hybri-dization was conducted after Poly(A)and biotin added to the RNA.Then,the chip was washed and dyed,and scanned thereafter.According to the differences in the expression profile of miRNAs and target gene analysis results,the miRNAs probably regulating the key gene of hindgut development was selected for target genes expression analysis.Results Compared with control group,expressions of 1 1 1 miRNAs in terminal hindgut of experimental group were up -regulated on the gestational day 1 6,and 1 1 7 miRNAs were down -regulated.Ten miRNAs of biggest differential expression be-tween them were selected for target genes prediction,pathway analysis,and Gene Ontology analysis.The results showed that some genes were closely related to rat fetus terminal hindgut growth and development,such as Shh,Hoxd13,and so on.According to the differential expression of miRNAs and target genes analysis,miR -1 93 might have an important role in the Hoxd13 gene for anorectal development.Real -Time PCR and Western blot showed that the expression of Hoxd13 and its protein level were significantly decreased when miR -1 93 highly expressed in rat intestinal epithelial cells,and the difference was statistically significant compared with the control group(1 .00 ±0.1 2 vs 0.71 ±0.1 0) (0.88 ±0.06 vs 0.75 ±0.08)(t =3.329,3.1 30;P =0.029,0.01 1 ).Conclusions miRNAs probably have an im-portant regulatory role in their target gene expression in terminal hindgut development of fetal rats,and miR -1 93 can significantly inhibit the expression of Hoxd13 gene in rat intestinal epithelial cells.

Objective To evaluate the performance of Sysmex XN‐9000 automated hematology analyzer .Methods According to international and domestic standards ,performance of analyzer was evaluated .Results The within‐batch and between‐batch preci‐sion ,carryover pollution rate ,linearity range and the accuracy of Sysmex XN‐9000 analyzer were all conform to related require‐ments .Leukocyte classification results compared with manual classification ,the correlation of neutrophil ,lymphocyte ,monocyte and eosinophil were fine ,but correlation of basophil was not very ideal .Conclusion The performance of Sysmex XN‐9000 analyzer could be satisfying ,could meet the needs of clinical inspection and diagnosis and treatment .

Objective To evaluate the value of CYRTA21-1,CEA and SPECT in diagnosis of bone metastasis in lung cancer.Methods 216 patients with lung cancer were examined by radionuclide bone imaging and serum CYFRA21-1,CEA.20 patients with benign lung disease were also studied.Results 121 of the 216 lung cancer patients were confirmed bone metastasis including 101 multi-metastasis and 20 singu- lar-metastasis. The positive rate was 56.02 %.The serum CYFR21-1,CEA level and positive rate of lung cancer patients with bone metastasis were obviously higher than those of patients without bone metastasis or with benign lung disease.The level of serum CYFR21-1,CEA had significant difference(P

Objective To evaluate the clinical feasibility of network live broadcast of digital subtraction angiography(DSA)video streaming.Methods DSA video streaming was captured by an advanced image capture board.MPEG-4 and Directshow framework were used for data compression and transmission.Data of DSA video streaming could be transmitted easily from server sender filter to client receiver filter according to TCP and UDP protocols.Images of 24 cases were captured,which were compared with images of DSA workstation by experienced doctors.The subiective evaluation criteria included the manifestition of normal and pathological blood vessels,and sharpness,contrast degree and real time efficiency of images.Results The delay time of live broadcast was less than one second in 100 M LAN.Among 24 cases,excellent imaging quality was got in 17 cases,good in 5 cases and midst in 2 cases.Conclusion Excellent images and synchronism of DSA video are achieved in this system.which can meet clinical requirements of diagnosis and synchronism.

Objective To investigate the relationship between IP_(3)/Ca~(2+)signal transduction process and the changes of intracellular calcium in detrusor cells in rats.Methods The model of overactive detrusor(OAD) was successfully established in female Wistar rats.Intracellular calcium fluorescence intensity of cultured detrusor cells in OAD rats and detrusor stability(DS) rats was measured by laser scanning confocal microscopy(LSCM).After pretreated by 10~(-5) mol/L IP_(3 )alone or with its inhibitor heparin,the intracellular calcium fluorescence intensity of cultured detrusor cells of DS rats in Ca~(2+)-contained and Ca~(2+)-free solutions was measured with LSCM.Results Intracellular calcium fluorescence intensity of cultured detrusor cells in OAD rats was stronger than that of DS rats(P