2.Investigate for the micro syndrome of HR-HPV cervical lesions
Cui XU ; Haitao HUANG ; Zhe JIN
International Journal of Traditional Chinese Medicine 2011;33(9):788-790
ObjectiveThrough the study of cervical lesions vagina inmage to investigate the micro-syndrome identified methods for cervical lesions. MethodsThrough the methods of retrospective study, collecting cases of HR-HPV infection (HC Ⅱ positive) and colposcopy detection, the objective evaluation of micro-syndrome differentiation was primarily established. ResultsRGB mode quantitative analysis for colposcopy images showed category 3 (the purple samples) occupied 44.4%, category 2 (the red samples)occupied 12.6%; category 1 (the white samples) occupied 43%. ConclusionsRGB mode quantitative analysis for colposcopy images was appropriate exploration of micro-differentiation in cervical lesions. There is a close relationship between traditional Chinese medicine syndrome and mucosai color differentiation by colposcopy.
3.Study on Chemical Constituents from Zingiberis Rhizoma
Zhe WANG ; Yongri JIN ; Xuwen LI
China Pharmacy 2017;28(24):3403-3405
OBJECTIVE:To study the chemical constituents of Zingiberis Rhizoma.METHODS:The compounds of Zingiberis Rhizoma were classified and purified by silica gel,Sephadex LH-20 column chromatography and thin layer chromatography (TLC).The structure of compounds were analyzed and identified according to chemical property and spectrum data.RESULTS & CONCLUSIONS:Five compounds were isolated from Zingiberis Rhizoma,i.e.methyl-6-gingerol (1),4-gingerol (2),β-eudesmol (3),2,5-dihydroxybisabola-3,10-diene (4),6-shogaol (5).The compounds 2,3,4 are isolated from Zingiberis Rhizoma for the first time.
4.Effect of Qingdushuan on PTEN-MDM2-p53 Network of Cervical Cancer SiHa Cell
Yanyan YU ; Zhe JIN ; Jiaoying LOU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(06):-
Objective To discuss the effect of Qingdushuan on SiHa cells of cervical cancer proliferation and protein expression in PTEN-MDM2-p53 network from the level of cell biology and molecular biology. Methods SiHa cells were cultivated with 4% containing serum. Normal control group, blank serum group, Qingdushuan group, Baofukang group and interferon group were established. The influence of inhibiting and proliferating SiHa cell was detected by MTT assay. Three protein expression was detected by Western blot method. Results After cultured with drug containing serum, the number of cells decreased, 4% concentration of serum for 72 h was the strongest. Compared with the control group, there were significant differences (P
5. Research and application of non-clay low damage temporary bridging drilling/completion fluids system
Academic Journal of Xi'an Jiaotong University 2009;21(3):176-183
Following the basic theory of protecting gas-reservoirs from damage with the temporary bridging technology, inert calcium carbonate (CaCO3) particles, whose diameter is consistent with the size of pores or apertures in the reservoir, were selected as the bridging agent, and modified resolvable starch was selected as filtration loss reducing particles to form the non-clay low damage temporary bridging drilling/completion fluids system (NLTDFS). Under the simulated condition of the well bottom during real drilling, NLTDFS was used to conduct dynamic and static damage experiments of cores for 48 hours, respectively, and then the experimented cores were permeated with pure nitrogen from the undamaged end to the damaged one to measure their recovery of permeability. The results showed that the permeability recovery rate of the core reached 90% or so, and the damaged depth was less than 1 cm, which demonstrates that NLTDFS has higher temporary bridging effectiveness and lower damage to the gas-reservoir than other drilling fluids system. NLTDFS has been used to drill many horizontal wells, and four of them have obtained high yield of natural gas. The yield of natural gas of LP1 well reached 85 × 104 m 3/day after completion with the rump pipe. The formation of the stable well wall and smooth drilling led to an API loss less than 4 mL and an HTHP loss less than 15 mL.
6. Research and application of non-clay low damage temporary bridging drilling/completion fluids system
Academic Journal of Xi'an Jiaotong University 2009;21(3):176-183
Following the basic theory of protecting gas-reservoirs from damage with the temporary bridging technology, inert calcium carbonate (CaCO3) particles, whose diameter is consistent with the size of pores or apertures in the reservoir, were selected as the bridging agent, and modified resolvable starch was selected as filtration loss reducing particles to form the non-clay low damage temporary bridging drilling/completion fluids system (NLTDFS). Under the simulated condition of the well bottom during real drilling, NLTDFS was used to conduct dynamic and static damage experiments of cores for 48 hours, respectively, and then the experimented cores were permeated with pure nitrogen from the undamaged end to the damaged one to measure their recovery of permeability. The results showed that the permeability recovery rate of the core reached 90% or so, and the damaged depth was less than 1 cm, which demonstrates that NLTDFS has higher temporary bridging effectiveness and lower damage to the gas-reservoir than other drilling fluids system. NLTDFS has been used to drill many horizontal wells, and four of them have obtained high yield of natural gas. The yield of natural gas of LP1 well reached 85 × 104 m 3/day after completion with the rump pipe. The formation of the stable well wall and smooth drilling led to an API loss less than 4 mL and an HTHP loss less than 15 mL.
8.A case of retroperitoneal fibrosis.
Xiang-Shan XU ; Yuan-Zhe JIN ; Qi WANG
Chinese Journal of Cardiology 2009;37(11):1047-1048
10.Affection of Zidan decoction containing serum on endometrial receptivity in uterine immunity environment
Qian CHEN ; Zhe JIN ; Qiudan LU ; Ying CAO ; Nan LI
International Journal of Traditional Chinese Medicine 2012;34(3):220-223
Objective To investigate the effect of Zidan decoction containing serum on endometrial receptivity in uterine immunity environment,and then to discuss if it's effect on endometrial receptivity through its controlling the expression of cell factors which related to endometrial receptivity.Methods Mice's endometrium tissue samples and uterine Natural Killer (UNK) cells were obtained from pregnant mice on gd 4.5 days and gd 12.5 days.After endometrial glandular epithelial cells,endometrial stromal cells and UNK cells were separated through primary culture,epithelial cells and endometrial stromal cells at rate of 1 ∶ 1 a certain concentration UNK were mixed together and highly purified-cultured.Different concentrations of Zidan decoction was acted on the culture solution.FBS,black and aspirin control group were setup for contrast.The expression of LIF and VEGF which influenced by Zidan decoction in the immunity environment was assayed by Realtime-PCR.Results The expression of LIF and VEGF was significantly increased in the immunity environment of uterine (in 10% Zidan decoction group,10% aspirin group and 10% blank control group:LIF was 2.10±0.20,1.98±0.14,0.90±0.05 separately,and VEGF was 3.17±0.31,2.52±0.09,0.92±0.06respectively,P<0.05).Conclusion Zidan decoction could improve endometrial receptivity by increasing the expression of cell factor which related to endometrial receptivity with the help ofUNK paracrine.