1.Gas Chromatography-Mass Spectrometry Analysis of Metabolic Profiling of Lung Tissues of Mice after Instillation of Fine Particulate Matter
Chunzhen SHI ; Xu MAO ; Xi HAN ; Chong FAN ; Meng JIN
Chinese Journal of Analytical Chemistry 2017;45(8):1116-1122
A method based on gas chromatography-mass spectrometry (GC-MS) was established to analyze the changes of intracellular metabolites and study the toxic mechanisms of different concentrations of particulate matter (PM2.5) effecting the lung tissues in mice.Nasal drip experiments of PM2.5 suspensions (0, 7.5, 20.0, 37.5 g/L) for mice were carried out, and the intracellular metabolites in lung tissues were extracted, pretreated and analyzed.Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were employed for pattern recognition, and an obvious distinction among different conditions was found.According to the PLS-DA loading diagram and variable important factor (VIP) value, 7 kinds of potential biomarkers, alanine, valine, leucine, ornithine, fumaric acid, citric acid and purine (p<0.01), were determined with significant differences between four different concentrations of PM2.5.Metabolic pathway analysis indicated that the oxidative stress reactions were enhanced, and the TCA cycle and the purine metabolism in lung cells were restrained after dripping PM2.5 to the lung tissues in mice.This study could provide a new perspective and theoretical basis for the further analysis on toxic mechanisms by PM2.5.
2.Electrophysiological changes in rat ventricular myocardium at different stages of experimental diabetes
Zhangqiang CHEN ; Shenjiang HU ; Juan DU ; Weiya SHI ; Xi JIN ; Yueliang SHEN ; Qian XIA
Chinese Journal of Pathophysiology 1986;0(02):-
AIM: To explore the probable mechanisms of diabetes-induced arrhythmias. METHODS: Diabetes was induced in male SD rats,using a single injection of alloxan into tail vein. Untreated age-matched animals were used as controls. All animals were observed by 2,4,6 and 8 weeks,respectively. Transmembrane potentials were recorded with conventional glass microelectrodes. RESULTS: Action potential duration(APD) at all level (APD10,APD20,APD30,APD50,APD70,APD90) was significantly lengthened in right ventricular papillary muscle from week 2 of diabetes. At week 8,APD was more lengthened at any level of repolarization than that at week 2. No differences were observed in the maximum rate of depolarization(V_ max ),overshoot(OS) and action potential amplitude(APA) as well as the resting membrane potential(RP) from the 2th to 8th week of diabetes. CONCLUSION: The results indicate that prolongation of APD may be prominently responsible for the increased incidence of cardiac re-entry-arrhythmias and sudden death,especially at late stages of diabetes.
4.Lead acetate induced DNA damage in blood lymphocytes of rats.
Jian-hua ZHOU ; Lian XUE ; Xi-jin SHI ; Liu-ming PENG ; Chen BIAN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(5):290-292
Animals
;
Comet Assay
;
DNA Damage
;
drug effects
;
Lymphocytes
;
drug effects
;
Male
;
Organometallic Compounds
;
toxicity
;
Rats
5.Expression of BSAP/CD30 in classic Hodgkin lymphoma using double-staining technique.
Yan-Feng XI ; Wen-Qi BAI ; Jin-Fen WANG ; Quan-Hong WANG ; Shi-Lan JIAO
Chinese Journal of Pathology 2007;36(2):136-137
Adolescent
;
Adult
;
Aged
;
Biomarkers, Tumor
;
metabolism
;
Child
;
Female
;
Gene Expression Regulation, Neoplastic
;
Hodgkin Disease
;
genetics
;
metabolism
;
Humans
;
Ki-1 Antigen
;
metabolism
;
Male
;
Middle Aged
;
PAX5 Transcription Factor
;
metabolism
;
Staining and Labeling
;
methods
;
Young Adult
6.Compare of complete and incomplete radical debridement for thoracolumbar spinal tuberculosis
Weidong JIN ; Qian WANG ; Zili WANG ; Jiandang SHI ; Guangqi GENG ; Xi ZHU ; Jialin ZHANG
Chinese Journal of Orthopaedics 2014;34(2):196-203
Objective To explore the compare of complete and incomplete radical debridement for thoracolumbar spinal tuberculosis.Methods Data of 296 patients with spinal tuberculosis from January 2000 to January 2011 were retrospectively analyzed.All patients were divided into two groups according to completeness of debridement:complete debridement group (group A) and incomplete debridement group (group B).There were 162 cases in group A including 86 males and 76 females,with an average age of 38.74± 17.26 years.There were 134 cases in group B including 73 males and 61 females,with an average age of 35.64± 18.21 years.All paticnts had undergone anterior debridement,focal graft implantation,anterior or posterior deformity correction,and internal fixation.Regular follow-up was required in the two groups.Results Residual sclerotic walls (36.54%),multipie cavities (34.62%),affected bony bridges (13.46%),sequestmm (3.37%),abscess (7.21%) and other lesionses (4.81%) were found in the group B.The first three factors were made up 84.62% of the total.The mean follow-up time was 76.13±8.32 months in the group A and 79.24±5.49 months in the group B.The symptoms,C-reactive protein and erythrocyte sedimentation rate were improved more obviously in group A than those in group B.Six months after operation,tuberculosis healing rate in group A and group B was 29.01% (47 patients) and 4.48% (6 patients),respectively.The mean healing time was 4.36± 1.27 months in the group A and 9.15±2.53 months in the group B,with significant differences.The mean the time of chemotherapy was 5.21± 1.38 months in the group A and (10.45±2.15) months in the group B,with significant differences.Reoperation rate in group A and group B was 0.62% (1/162) and 4.48% (6/134),respectively.Conclusion Sclerotic bone,multiple cavities,and bony bridges are parts of foci in spinal tuberculosis.Clearing tuberculous foci with sclerotic bone,multiple cavities,and bony bridges can increase the curative effect,shorten the time of chemotherapy and reduce the side effects of drug,thus early resumption can be achieved.
7.Antisense oligonucleotide mediated inhibition on telomerase activity in gallbladder carcinoma cell
Jin Bin ; JINAG Xi-hong ; WANG Wei ; XU Ke-sen ; SHI Zhao-hui
Chinese Journal of Current Advances in General Surgery 2005;8(1):27-30
Objective:To study the antisense oligonucleotide mediated inhibition on telomerase activity and cell proliferation of GBC-SD cell.Methods:We design the antisense,sense,and random oligonucleotide with phosphoric acid modification for the hTR(Human Telomerase RNA)template sequence.MTT and PCR methods were used to observe the inhibition on telomerase activity and cell proliferation of GBC-SD cell ,and fibroblast cells were used as control group.Results:PS-ODN can lead to the reduction of cell survival rate of GBC-SD cell,wich dosage dependence.Tne experimental group cell detected by scanning electron appeared apoptotic feature.Conclusion:PS-ODN can inhibit telomerase activity of GBC-SD cell effectively and induce the cell apoptosis.
8. Relationship between intestinal mucosa associated microbiota and clinical manifestations in the patients with ulcerative colitis
Shenhao WANG ; Wenting ZHONG ; Xiaolan LU ; Haitao SHI ; Gang ZHAO ; Jing ZHANG ; Yaofeng JIN
Chinese Journal of Digestion 2018;38(11):774-779
Objective:
To investigate the changes of the intestinal mucosa-associated microbiota in the patients with ulcerative colitis (UC), and to explore their correlation with the clinical manifestations.
Methods:
From June to October 2016, at Gastrointestinal Endoscopy Center, the Second Affiliated Hospital of Xi′an Jiaotong University, 28 patients with UC and 16 healthy individuals who underwent colonoscopy examination were enrolled. The mucosa specimens of them were collected for fluorescent
9.Flow cytometry combined assay for phosphatidylserine and CD62p expressed by preserved platelets.
Xi-Lin OUYANG ; Jing-Han LIU ; Qun LUO ; Qun SHI ; Wei HAN ; Xi-Jin LI ; Dayong GAO
Journal of Experimental Hematology 2002;10(1):66-69
Human platelets have distinct characters when preserved by different methods. A efficient flow cytometric assay for different preserved platelets expression of CD62p and phosphatidylserine(PS) is in dire need. Efficient flow cytometric assay for CD62p and PS expressed by preserved platelets was established and the major conditions were optimized. The platelets need not to be washed to wipe off plasma and can be labelled diredtly during the sample preparation. It is efficient for flow cytometric analysis when fresh platelet riched plasma (FPRP) was set as negative control, thrombin actived FPRP, and liquid nitrogen treated FPRP were set as positive control respectively. Gly-Pro-Arg-Pro acetate salt (GPRP) was applied to prevent platelets aggregation and fibrin formation, stabilize platelets and minimize the artificial platelets activation. This is also the key to conquer difficulty of flow cytometric quantitive analysis when platelet, Ca(2+) and plasma coexist. This flow cytometric method is specially suitable for the multi-parameter assay including PS expression for cryopreserved platelets. Minimal sample manipulation, no fixation, and GPRP application resulted in minor artifacts and good sample stability. Results suggested, this flow cytometric assay for preserved platelets is simple and efficient. In addition, the author prepared four different methods treated platelets that can be easily distinguished through this flow cytometric assay. It not only makes sure the practicability of this flow cytometric assay, but also suggests the value of the treated platelets applied in preserved platelets flow cytometric ass
Blood Platelets
;
metabolism
;
Flow Cytometry
;
methods
;
Humans
;
P-Selectin
;
biosynthesis
;
Phosphatidylserines
;
analysis
;
Reproducibility of Results
;
Tissue Preservation
10.Effects of langchuangjing granule on apoptotic of CD4+ T and CD19+ B in spleen of BXSB mice with systemic lupus erythematosus.
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(9):692-694
OBJECTIVETo study the effect on apoptotic of CD4+ T, CD19+ B in spleen of BXSB mice with systemic lupus erythematosus treated with Langchuangjing Granule and to probe into the mechanism of treatment.
METHODSThe apoptosis was examined by the flow cytometric analysis and immunofluorescence double-staining method.
RESULTSApoptosis of male BXSB mice speeds up. Langchuangjing Granule can restrain the excessive apoptosis of CD4+ T and CD19+ B cells in spleen.
CONCLUSIONLangchuangjing Granule treated systemic lupus erythematosus by restraining the excessive apoptotic of T, B lymphocytes, probably restraining the release of excessive amount of apoptotic DNA fragments, so decreasing abnormal proliferation of B cells and the produce of autoantibodies.
Animals ; Antigens, CD19 ; analysis ; Apoptosis ; drug effects ; B-Lymphocytes ; pathology ; CD4-Positive T-Lymphocytes ; pathology ; Drugs, Chinese Herbal ; pharmacology ; Female ; Lupus Erythematosus, Systemic ; immunology ; pathology ; Male ; Mice ; Mice, Inbred Strains ; Random Allocation ; Spleen ; pathology