Objective To explore the clinical effect of high-frequency repetitive transcranial magnetic stimulation (rTMS) on post-stroke depression (PSD). Methods Sixty PSD patients were randomly divided into an rTMS group and a control group, each of 30 patients. In addition to conventional treatment, rTMS group members were given high-frequency rTMS treatment for 10 d while the control group were given sham stimulation. In order to test the efficacy, the two groups were compared using the Hamilton depression rating scale (HAMD) before and after 10 and 30 days treatment. Results After 10 days of treatment the rTMS group had significantly lower HAMD scores than before treatment, and average HAMD scores differed significantly between the rTMS group and the controls, with the former performing significantly better. There was no obvious difference in HAMD scores after 30 days of treatment between the two groups. Conclusions High-frequency rTMS is a safe and effective treatment for PSD.

37% TBSA third degree burns was inflicted to rats and they were decapitated on the third day postburn.The 3H-proline absorption ratio of the everted small intestine of the burn group (1.87?0.27) was significantly lower than that of the control group (2.35?0.49) In the burn group,the ATPase activity of the small intestinal mucosa was 5.61?2.27 nmole/min/mg protein,and the Na+-K+-ATPase activity was 2.86?0.83 nmole/min/mg protein,while they were 9.63?1.04 nmole/min/mg protein and 5.36?0.95 nmole/min/mg protein in the control group respectively.Thus,it was apparent that the two activities decreased markedly in the rats with burn injury It was found that the amino acid absorption ratio was positively correlated with the activity of Na+-K+-ATPase of the small intestine mucosa in the burn group as well in the control group.The relative coefficient was 0.744 p(1) 0.025 in the control,and 0.776 p(1) 0.025 in the burn group.It suggests that the decrease of amino acid absorption ratio in the burn group is likely due to the decreased activity of Na+-K+-ATPase activity.

OBJECTIVE:To guide the formulation of emergency management plan for pharmacy department and reduce emer-gency risk. METHODS:By the methods of risk integration,considering the frequency and severity of the hazard events,the risk integral value of emergency events was calculated and hazard level was judged. RESULTS:The top three hazard events of pharma-cy department were information system failures (75.21),fire (63.00) and cytotoxic drugs overflow (62.21). All of the above events were moderate risk. According to the results of this evaluation,pharmacy department targeted emergency drills,in order to improve the ability to cope with risks. CONCLUSIONS:In order to improve the ability of facing and dealing hazard events,disas-ter vulnerability analysis is the base of developing and exercising emergency plans.

Humans ; Stress, Physiological ; Takotsubo Cardiomyopathy ; diagnosis ; therapy

Abstract?AlM: To evaluate the difference of contrast sensitivity ( CS) in photopic and scotopic environments in eyes with myopia and myopic astigmatism operated with femtosecond laser in situ keratomileusis ( femto-LASlK ) and laser in situ keratomileusis ( LASlK) .?METHODS: ln a prospective study 160 myopia and myopic astigmatism patients' eyes were involved, which accepted femto- LASlK or LASlK in our hospital from January 2010 to February 2012. The myopia degree was -1. 50 ~ -10. 00D, the astigmatism degree ≤-6. 0D. Eighty eyes were treated with femto-LASlK in group A, and 80 eyes were treated with LASlK in group B, All patients in the treatment group completed the final 6mo of follow-up. The uncorrected visual acuity ( UCVA) and the best spectacle-corrected visual acuity ( BSCVA) , objective and manifest refractions, results of slit-lamp examination, the side effects, intraocular pressure, corneal topography, CS in photopic and scotopic environments were noted.? RESULTS: All of operations on 160 cases were successful without severe complication after 6 mo follow-up. CS of femto-LASlK group ( group A) at each spatial frequency environment were higher than that of LASlK group (group B). ln group A, after 1mo the photopic CS, after 3mo of scotopic CS recovered to the preoperative level, 6mo after surgery improved than before the operation. ln group B, after 3mo photopic CS to the preoperative level, scotopic CS at 6mo after operation was still not recovered to the preoperative level.?CONCLUSlON:Femto-LASlK for correction of myopia and myopic astigmatism, in improving the postoperative contrast sensitivity under shade environment has more advantages than LASlK.

OBJECTIVE: To improve the oral bioavailability of glycyrrhizin by preparing glyeyrrhizin-sodium deoxycholate/phos-pholipid-mixed micelles (GL-SDC/PL-MMs). METHODS: GL-SDC/PL-MMs was prepared by a film dispersion method. In order toevaluate the property GL-SDC/PL-MMs comprehensively, physical and chemical properties determination, in situ intestinal absorption, and pharmacokinetics test were carried out. RESULTS: The average particle size of drug-loaded micelles prepared by film dispersion method was (82.99±7.5) nm and Zeta potential was (-32.23±1.05) mV. Compared with the control drug, glycyrrhizin loaded in SDC/PL-MMs significantly improved its in situ intestinal absorption. The oral bioavailability was markedly enhanced, indicated by the increase of ρmax to 77.26 μg · mL-1, which was 2.82 times of that of the control drug. CONCLUSION: SDC/PL-MMs is expected to be developed into a new drug delivery systems of GL.

OBJECTIVE: To determinate triptolide and wilforlide A in biological samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and to verify the method. METHODS: After 0.4 mL blood, urine or 0.4 g hepatic tissues with internal standard were extracted by ethyl acetate, they were separated on a Allure PFP Propyl (100 mm x 2.1 mm, 5 µm) with a mobile phase of methanol-20 mmol/L ammonium acetate using gradient elution. For mass spectrometric detection, electrospray ionization (ESI⁺) in positive mode was elected and the data was collected using multiple-reaction monitoring (MRM). RESULTS: The linearity was good (r > 0.995 0) and the limit of detection was 2 ng/mL or 2 ng/g for triptolide and wilforlide A. The recovery was 61.08%-102.98%. The intra-day and inter-day precision was less than 12.58% for each biological sample, and the accuracy was 90.61%-105.80%. CONCLUSION This method is simple, convenient and good selective, and could be applied to analysis of triptolide and wilforlide A in different biological samples. And the method may provide technical support for forensic medicine identification, clinical diagnosis and treatment of tripterygium wilfordii Hook. f. poisoning.
Chromatography, High Pressure Liquid ; Diterpenes/urine* ; Epoxy Compounds/urine* ; Humans ; Oleanolic Acid/urine* ; Phenanthrenes/urine* ; Sensitivity and Specificity ; Tandem Mass Spectrometry

In order to make students have integrative qualities and reform the shortage of former teaching methods in the food microbiology experiment,a new experiment teaching system was established,introducina the integrative and innovative experiment to the students gradually.The reformation of experiment in food microbiology has trained the students well and improved their special skills.Most important of all,it can offer them a chance to realize their own ideas.The students can design an innovative and integrative experiment to carry out their originalities.Good effects have been made since this system is very helpful to improve their consciousness of innovation and integrative abilities in doing experiments.

CD2 Antigens ; metabolism ; CD3 Complex ; metabolism ; Epstein-Barr Virus Infections ; Hematopoietic Stem Cell Transplantation ; Herpesvirus 4, Human ; isolation & purification ; Humans ; Killer Cells, Natural ; pathology ; virology ; Lymphoproliferative Disorders ; metabolism ; pathology ; therapy ; virology ; Poly(A)-Binding Proteins ; metabolism ; Prognosis ; T-Cell Intracellular Antigen-1

The effects of continuous subcutaneous insulin injection (CSII, n=306) and multiple subcutaneous insulin injection (MSII, n=310)on hyperglycemia in patients with diabetes mellitus were compared. The results suggest that the effect of CSII is more prompt and efficient with lower incidence of hypoglycemia as compared with MSII.