1.Somatic Mutational Analysis of MEN1 and Phenotypic Correlation in Sporadic Parathyroid Tumors .
Young Su CHAE ; Hee Jin KIM ; Sun Wook KIM ; Myung Chul CHANG
Journal of the Korean Surgical Society 2009;76(1):15-22
PURPOSE: MEN1 gene mutation causes multiple endocrine neoplasia type 1. It also suggests that somatic MEN1 gene mutation plays a role in sporadic endocrine tumor. In this study, we examined whether somatic mutations of MEN1 gene are responsible for sporadic parathyroid tumors and correlate with clinical manifestations of parathyroid tumors. METHODS: Somatic mutation of MEN1 gene in the formalin-fixed, paraffin-embedded parathyroid tumor tissue from 8 adenomas, 2 carcinomas and 1 hyperplasia were analyzed by direct sequencing. Clinicopathological parameters were reviewed from medical records and compared with the mutational data. RESULTS: Eight of eleven (73%) sporadic parathyroid tumors had somatic MEN1 mutations of 14 different types. In the 14 types, 13 were a point mutation which is composed of 8 missense mutations, 2 nonsense mutations and 3 silent mutations. One of 14 types is a frameshift deletion of 27 base pairs in exon 2. Somatic mutation was frequent in the exon 2 and exon 10. Four types of polymorphism were found. There was no correlation between the presence of mutations and clinicopathological phenotype of parathyroid tumors. CONCLUSION: This result suggests that somatic mutation of MEN1 gene plays a definite role in sporadic parathyroid tumor formation.
Adenoma
;
Base Pairing
;
Codon, Nonsense
;
Exons
;
Hyperplasia
;
Medical Records
;
Multiple Endocrine Neoplasia Type 1
;
Mutation, Missense
;
Phenotype
;
Point Mutation
2.T Cell-specific Immunosuppression Using Tautomycetin or PTD-conjugated Protein Drugs.
Wook Jin CHAE ; Je Min CHOI ; Jung Jin YANG ; Sang Kyou LEE
Yonsei Medical Journal 2004;45(6):978-990
No abstract available.
Animals
;
Antibiotics, Antifungal/*therapeutic use
;
Humans
;
Immunosuppression/*methods
;
Protein Structure, Tertiary
;
T-Lymphocytes/*immunology
;
*Transduction, Genetic
3.Self-expandable Graft Stenting in an Iatrogenic Fistula between Common Carotid Artery and Internal Jugular Vein.
Sung Chul JIN ; Dong Hyuk LEE ; Chae Wook HUH
Journal of Cerebrovascular and Endovascular Neurosurgery 2017;19(3):213-216
A 47-year-old woman with stage IV chronic kidney disease developed a fistula between common carotid artery (CCA) and internal jugular vein (IJV) during central catheterization of the right IJV. The patient was treated with a self-expandable graft stent, which achieved successful closure of the carotid-jugular fistula. As demonstrated in our case, self-expandable graft stents could be a feasible treatment option for CCA-IJV fistulas without additional interventional procedures such as balloon angioplasty.
Angioplasty, Balloon
;
Carotid Artery, Common*
;
Catheterization, Central Venous
;
Female
;
Fistula*
;
Humans
;
Jugular Veins*
;
Middle Aged
;
Renal Insufficiency, Chronic
;
Stents*
;
Transplants*
4.Three Cases of Anti-LW Antibody Identification at a Tertiary Hospital in Korea
Seungwan CHAE ; Kyoung Bo KIM ; Haein YU ; Hwa Jin CHOI ; Dong Wook JEKARL ; Jihyang LIM ; Yonggoo KIM
Korean Journal of Blood Transfusion 2022;33(1):39-45
The Landsteiner–Wiener (LW) antigen is a type of red blood cell antigen. Anti-LW appears in various situations, including alloantibodies, autoantibodies, and even transiently occurring antibodies. Anti-LW has similar characteristics to anti-D, so it can interfere with interpreting pre-transfusion tests and finding compatible blood. This paper introduces three cases in whom anti-LW was detected through antibody identification tests. All three cases were examined using the column agglutination technique with ID-DiaPanel (Bio-Rad, Hercules, CA, USA) on a LISS/Coombs card, ID-DiaPanel p (Bio-Rad) on a NaCl/Enzyme card, and ID-DiaPanel (Bio-Rad) on a LISS/Coombs card using red blood cells treated with dithiothreitol. The auto-control test, direct antiglobulin test, and umbilical cord blood test were also performed. In all three cases, the reaction with D-positive panel cells was stronger than that with the D-negative panel cells, and two of them showed a pan-agglutinated reaction in ID-DiaPanel p (Bio-Rad) with NaCl/Enzyme card. They were reported as anti-LW, and as in these cases, anti-LW can occur under a range of conditions and interfere with proper transfusion. Therefore, it is important to identify anti-LW accurately, and if anti-LW is present, the transfusion of D-negative ABO matched blood should be recommended because of the low expression of the LW-antigen. On the other hand, D-positive blood is not a contraindication when an urgent transfusion is needed.
5.Relationships among Depression, Self-rated Health, Health Behaviors and Drug Abuse by Job Status.
Su Mi CHAE ; Jin Wook JEONG ; Sang Young LEE ; Kyung Hwa HEO
Korean Journal of Occupational Health Nursing 2015;24(3):245-257
PURPOSE: This study was performed to investigate the present condition of drug abuse and its association with depression, self-rated health and health behaviors by job status in Korean adults. METHODS: Data were derived from the study on four addiction problem and suicide in 2014. Multiple logistic regression was used to analyze patterns of drug abuse according to depression, self-rated health and health behaviors. RESULTS: The prevalence of drug abuse during the past year was 17.1% of the 4,018 subjects. About 3.3 times risk for drug abuse was found among individuals who had high depression scores. The risk of drug abuse was higher among those who were smoking (OR:1.46, 95% CI:1.17~1.83), drinking more frequently (OR:1.30, 95% CI:1.07~1.58), sleeping insufficiently (OR:1.31, 95% CI:1.03~1.67), eating irregularly (OR:1.45, 95% CI:1.19~1.76). Drug abuse problem was detected more seriously among employed than unemployed adults. CONCLUSION: Health-related behaviors, such as smoking, drinking, sleeping, eating should be considered simultaneously when designing strategies to deal with drug abuse problem, and it is important to understand the interaction between drug abuse and mental health. Furthermore, workplace based intervention can be effective in solving drug abuse problem.
Adult
;
Depression*
;
Drinking
;
Eating
;
Health Behavior*
;
Humans
;
Logistic Models
;
Mental Health
;
Prevalence
;
Smoke
;
Smoking
;
Substance-Related Disorders*
;
Suicide
6.A Case of Propionic Acidemia.
Woo Seop YEOUM ; Kwang Wook LEE ; Byeong Ho CHAE ; Baek Keun LIM ; Hong Jin LEE
Journal of the Korean Pediatric Society 1999;42(6):901-907
Propionic acidemia is an autosomal-recessive inborn error of branched-chain amino acid metabolism. It is caused by deficient activity of propionyl-coenzyme A carboxylase and is characterized by a spectrum of clinical and biochemical findings. It usually manifests in the neonatal period or early infancy. Since Childs et al first described the propionic acidemia of infants in 1961, it has rarely been reported. There have been no previous report of this organic acidemia in Korea. We present a case of propionic acidemia in a 4-day old male, who had poor feeding, dehydration, and hyperammonemia and died at 12 days of age. Diagnosis was established by gas chromatography and mass spectrometry, and this case is the first reported propionic acidemia in literature in Korea. A review of the related literature was included.
Child
;
Chromatography, Gas
;
Dehydration
;
Diagnosis
;
Diethylpropion*
;
Humans
;
Hyperammonemia
;
Infant
;
Korea
;
Male
;
Mass Spectrometry
;
Metabolism
;
Methylmalonyl-CoA Decarboxylase
;
Propionic Acidemia*
7.A Case of Pulmonary Inflammatory Pseudotumor.
Hong KOH ; Hyun Wook CHAE ; Su Jin LEE ; Bong Shik YOON ; Gwang Cheon JANG ; Kyung Hoon KANG ; Jeong Hae KIE
Pediatric Allergy and Respiratory Disease 2006;16(3):248-252
Inflammatory pseudotumor of the lung is considered to be a rare, benign, neoplastic lesion, consisting mainly of spindle mesenchymal cells, sometimes in such a way that its histological appearance mimics that of a spindle cell sarcoma, fibrous histiocytoma or fibrosarcoma. A case of inflammatory pseudotumor of the lung occurring in a 13-year-old boy is reported with pathologic findings, including its ultrastructure. The patient had had no symptoms and accidentally discovered his condition after a chest X-ray examination at a regular school physical check up. The mass was located in the suprahilar area of the left lung. Exploratory thoracotomy revealed a large mass that was removed, together with the left upper lobe of the lung. Microscopically, the mass was composed of numerous interstitial inflammatory cells, mainly lymphoplasma cells. Ultrastructurally, the spindle-shaped mesenchymal cells were arranged haphazadly and the normal pulmonary structure was nearly totally destroyed. Emphasis is given to complete resection of the tumor for both diagnostic and therapeutic purposes.
Adolescent
;
Child
;
Fibrosarcoma
;
Granuloma, Plasma Cell
;
Histiocytoma, Benign Fibrous
;
Humans
;
Lung
;
Male
;
Plasma Cell Granuloma, Pulmonary*
;
Sarcoma
;
Thoracotomy
;
Thorax
8.Analysis of Twenty-Three Cases of Cytomegalovirus Disease.
Jin Wook KIM ; Dong Hyun SHIN ; Myoung Don OH ; Hyun Chae JUNG ; Woo Ho KIM ; In Sung SONG ; Kang Won CHOE
Korean Journal of Infectious Diseases 1998;30(3):251-258
BACKGROUND: Cytomegalovirus(CMV) disease is an important opportunistic infection and contributes to significant morbidity and mortality in immunocompromised hosts. To determine predisposing conditions to CMV disease and its prognosis, the authors reviewed the clinical courses of patients with CMV disease. METHODS: We reviewed medical records of 23 patients with CMV disease diagnosed at Seoul National University Hospital from 1987 to 1997. RESULTS: CMV pneumonia was diagnosed in 8 patients. Underlying conditions of the patients were allogeneic bone marrow transplant(BMT) in 4 patients, lupus nephritis in 1, dermatomyositis in 1, and renal transplantation recipient in 1. The cumulative dose of corticosteroid given to the BMT recipients before the development of CMV pneumonia ranged between 1,000 and 4,700 mg, whereas that to the non-BMT patients ranged between 2,100 and 6,000 mg. Of the 8 patients with CMV pneumonia, five patients(75%) died. Of the 15 CMV gastroenteritis, two had CMV gastric ulcers. The gastric ulcers showed clinical and endoscopic improvement with systemic ganciclovir therapy. Among 13 patients with CMV enterocolitis, nine had preceding GI diseases, and nine had received systemic corticosteroids. Five patients died. The mortality was lower in patients with underlying bowel diseases than in those without ones(22% vs. 75%, respectively). CONCLUSION: The mortality of CMV disease was 62.5%, 0%, and 38% in patients with CMV pneumonia, gastric ulcer, and enterocolitis, respectively. GVHD, systemic corticosteroid and/or immunosuppressive therapy were major risk factors of CMV diseases.
Adrenal Cortex Hormones
;
Bone Marrow
;
Cytomegalovirus*
;
Dermatomyositis
;
Enterocolitis
;
Ganciclovir
;
Gastroenteritis
;
Humans
;
Immunocompromised Host
;
Kidney Transplantation
;
Lupus Nephritis
;
Medical Records
;
Mortality
;
Opportunistic Infections
;
Pneumonia
;
Prognosis
;
Risk Factors
;
Seoul
;
Stomach Ulcer
9.Prevalence of PPNG in Seoul ( 1993 ).
Jae Hong KIM ; Jin Bok KIM ; Chul Wook KWON ; Young Tae KIM ; Joong Hwan KIM ; Chae Soo KIM ; Sung In CHO ; Hak Kyu LEE
Korean Journal of Dermatology 1995;33(2):275-279
BACKGROUND: In recent years gonorrhea has been panendemic and remains one of the most common STDs in the world, especially in developing countries. OBJECTIVE & METHOD: For the detection of a more effective teripeutic regimen and assessing the prevalence of PPNG, will have been trying to study the paieits who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. RESULTS: In 1993, 187 strains of N. gonorrhoeae were isolated, ar, ong which 139(74.3%) were PPNG. CONCLUSION: Our results suggests that the prevalence of PPNG in Seoul is still increasing.
Developing Countries
;
Gonorrhea
;
Prevalence*
;
Public Health
;
Seoul*
;
Sexually Transmitted Diseases
10.Heart-Model-Based Automated Method for Left Ventricular Measurements in Cardiac MR: Comparison with Manual and Semi-automated Methods.
Seung Hoon CHAE ; Whal LEE ; Eun Ah PARK ; Jin Wook CHUNG
Journal of the Korean Society of Magnetic Resonance in Medicine 2013;17(3):200-206
PURPOSE: To assess the effect of applying an automated heart model based measurements of left ventricle (LV) and compare with manual and semi-automated measurements at Cardiovascular MR Imaging. MATERIALS AND METHODS: Sixty-two patients who underwent cardiac 1.5T MR imaging were included. Steady state free precession cine images of 20 phases per cardiac cycle were obtained in short axis views and both 2-chamber and 4-chamber views. Epicardial and endocardial contours were drawn in manual, automated, and semi-automated ways. Based on these acquired contour sets, the end-diastolic (ED) and end-systolic (ES) volumes, ejection fraction (EF), systolic volume (SV) and LV mass were calculated and compared. RESULTS: In EDV and ESV, the differences among three measurement methods were not statistically significant (P = .399 and .145, respectively). However, in EF, SV, and LV mass, the differences were statistically significant (P=.001, <001, <001, respectively) and the measured value from automated method tend to be consistently higher than the values from other two methods. CONCLUSION: An automatic heart model-based method grossly overestimate EF, SV and LV mass compared with manual or semi-automated methods. Even though the method saves a considerable amount of efforts, further manual adjustment should be considered in critical clinical cases.
Axis, Cervical Vertebra
;
Heart
;
Heart Ventricles
;
Humans