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3.Studies on the VP4 and VP7 Genes of Bovine Rotaviruses from Field Samples Using RT-PCR and RFLP Analysis.

Seong Jin JEON ; Shien Young KANG ; Chung Ho CHANG ; Chung Won CHUNG ; Won Yong KIM

Journal of the Korean Society of Virology 1998;28(2):165-174

Characterizations of the VP4 (P type) and VP7 (G type) genes of Korean isolates of bovine rotavirus were performed using RT-PCR/RFLP and nucleotide sequencing analysis. After RT-PCR amplification of partial length (1094bp) of the VP4 and full length (1062bp) of the VP7 genes, amplified PCR products were digested with restriction endonucleases and digestion patterns were compared with those of reference rotaviruses. With the VP4 genes, four RFLP (AD) profiles were observed; three (A, B and C) were the same as those of bovine rotavirus NCDV (P[1]), IND (P[5]) and B223 (P[11]), respectively, Profile D was the same as that of porcine rotavirus OSU (p[7]). With the VP7 genes, five RFLP profiles (I-V) were observed; three of them (1, II and III) were the same as those of bovine rotavirus NCDV (G6), Cody I-801 (G8), and B223 (G10), respectively, Profile IV and V were atypical to those of reference bovine rotaviruses used in this study. These two profiles were identified as G6 and G5, respectively, after analyzing and comparing the nucleotide sequences. The G typing analysis revealed that 61.9% (26/42) were G6, which included G6 subtype; 28.6% (12/42) were G5; 7.1% (3/42) were G10; 2.4% (1/42) were G8. The P typing analysis revealed that 54.8% (23/42) were P(5); 28.6% (12/42) were P(7); 11.8% (5/42) were P(11); 4.8% (2/42) were P(1). Our results showed that G6/P(5) were the most prevalent rotaviruses in diarrheic calves in Korea. Also, this is the first report that G5P(7) rotaviruses were identified from cattle with diarrhea.
Animals ; Base Sequence ; Cattle ; Diarrhea ; Digestion ; DNA Restriction Enzymes ; Korea ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length* ; Rotavirus*

10.Chromosome aberration and glutathio -S- transferase activitiy in peripheral lymphocytes of workers exposed to ethylene oxide.

Jin Sook KIM ; Sung Eun LEE ; Hai Won CHUNG

Korean Journal of Epidemiology 1993;15(2):212-221

1.Severe Acute Respiratory Syndrome.

2.An epidemiological study on temporomandibular disorders in young adult females.

3.Studies on the VP4 and VP7 Genes of Bovine Rotaviruses from Field Samples Using RT-PCR and RFLP Analysis.

4.A clinical review of 1292 cases of burn patients.

5.A clinical review of 1292 cases of burn patients.

6.Incidence of sepsis associated with total parenteral nutrition solutions made in the nursery and pharmacy.

7.A Case of Mucocutaneous Lymph Node Syndrome Complicated by Partial Intestinal Obstruction.

8.Effect of Clinical Improvement of Schizophrenic Symptoms on 99m Tc-HMPAO Brain SPECT.

9.Evaluation for the sensitivity of LISS in antiglobulin test.

10.Chromosome aberration and glutathio -S- transferase activitiy in peripheral lymphocytes of workers exposed to ethylene oxide.

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