Objective To observe the effect of several antihistamines,including epinastine,on the release of leukotriene B4(LTB4),interleukin 4(IL-4)and interleukin 5(IL-5)from spleen lymphocytes of allergic mice challenged by ovalbumin(OVA).Methods OVA-sensitized BALB/c mice were used as animal model of allergy.Spleen lymphocytes isolated from these mice were treated with epinastine(0.1,1.0,10 μmol/L),ebastine(10 μmol/L),cetirizine(10 μmol/L),and dexamethasone(1.0 μmol/L),respectively,followed by incubation with OVA.Those cells without pretreatment with antihistamines served as the control.One hour after the challenge,enzyme-linked immunosorbent assay(ELISA)was applied to measure the level of LTB4,IL-4 and IL-5 in the cell culture supernates.Results Following the incubation with OVA,the levels of LTB4,IL-4 and IL-5 increased significantly in culture supernates of control spleen lymphocytes.Epinastine significantly inhibited the release of LTB4,IL-4 and IL-5 in a dose-dependent manner.At the concentration of 10 μmol/L,the inhibitory effect of epinastine was more potent than that of ebastine and cetirizine for IL-5 and LTB4 release(all P＜0.05):for IL-4,the efrect of epinastine was similar to that of ebastine (P＞0.05)but more potent than that of cetirizine(P＜0.05).Conclusion Epinastine has a potent anti-inflammatory effect.

Objective To assess the cerebral protective effects of different doses of propofol during mild hypothermic cardiopulmonary bypass (32℃-35℃) .Methods Thirty consecutive ASA Ⅱ-Ⅲ patients aged 18-50 years, undergoing elective cardiac surgery under mild hypothermic CPB were randomly divided into 3 groups: in group Ⅰ and Ⅱ the patients were given a bolus of propofol 1 mg kg-1 at the beginning of CPB followed by propofol infusion at a rate of 3 mg ? kg -1? h -1 (group Ⅰ ) or 6 mg? kg-1 ?h-1 (group D ) till the end of CPB and in group Ⅲthe patients received midazolam infusion at 0.2 mg? kg-1 h-1 instead of propofol. Anesthesia was induced with intravenous midazolam 0.15 mgkg-1 ,fentanyl 5 ?g kg-1 and vecuronium 0.1 mg? kg-1 .After tracheal intubation fentanyl 30-50 ?g kg-1 was infused during CPB and muscle relaxation was maintained with intermittent iv boluses of vecuronium. Surgery was performed with mild hypothermic CPB(32℃-35℃ ) .Arterial blood pH and PaCO2 were maintained within normal range and MAP was maintained at 50-80 mm Hg during CPB. Hematocrit was maintained at 25%-30% .Internal jugular vein(IJV) was cannulated and the catheter was advanced retrogradely till jugular bulb. Naso-pharyngeal temperature was monitored. Blood samples were taken simultaneously from artery and UV befor CPB (A) ,when mild hypothermia was being stably maintained(B) ,when the patients were rewarmed to 361 (C)and 30min(D),4-6 h(E)and 24 h(F) after discontinuation of CPB, for determination of cerebral oxygen metabolism and balance, cerebral lactate production (CLP), blood malondialdehyde (MDA) and superoxide dismutase (SOD) levels. CLP was calculated from arterial-cerebral venous blood lactate difference /arterial blood lactate concentration. Lactate oxygen index (LOI) was calculated from arterial-cerebral venous blood lactate difference /arterial-cerebral venous blood O2 content difference.Results In group I (low propofol dose) 30% patients developed cerebral O2 inbalance during rewarming but blood MDA, CLP and LOI were decreased. In midazolam group (group Ⅲ) cerebral O2 balance was improved but there was no depression of blood MDA, CLP and LOI. In groupⅡ (high propofol dose) cerebral oxygenation was improved and blood MDA level, CLP and LOI were significantly decreased.Conclusion The study shows that propofol infusion at a rate of 6 mg?kg-1? h-1 during CPB can improve cerebral oxygenaton, inhibit blood MDA level, CLP and LOI, and plays a role in the cerebral protection during cardiac surgery with CPB, but more vasoconstrictors are needed.

Objective To evaluate the effects of IL-1? and combination of IL-1?,IL-11,LIF and GDNF on inducing human neural stem cells(hNSCs)to differentiate into dopaminergic(DA)neurons in vitro. Methods A great deal of neurospheres was obtained by the technology of serum-free culturing and single-cell cloning,and was splitted into 3 groups,which were cultured in different media.In control group,the differentiation medium used only contained 10%FBS.In IL-1? group,the medium contained IL-1? and 10%FBS.In united factors group,the medium contained IL-1?,10%FBS supplemental with IL-11,LIF and GDNF.After 3 weeks,the tyrosine hydroxylase(TH)positive cells were detected by using immunofluorescence,and image processing about the number,the induced differentiational rate,the cell bodies' areas and the cells' perimeters of TH positive neurons was carried out.TH/MAP-2 double-immunofluorescence labeling was used to calculate the percentage of DA neurons in total neurons. Results In the control group,there were few TH positive neurons with poorly developed morphology.The presence of IL-1? induced more DA neurons,but these cells were still immature.In the united factors group,the number of maturer TH positive DA neurons was the most. Conclusion IL-1? has an obvious effect on inducing hNSCs derived from human fetal mescenphalon to DA neurons.The utilization of IL-1?,IL-11,LIF and GDNF in combination has a cooperative effect on inducing differentiation of hNSCs to mature DA neurons.

Objective To study the effect of Danxuan Koukang(DXKK) drug serum on proliferation and proliferating cell nuclear antigen(PCNA) production of human oral mucosal fibroblasts(FB) induced by areca nut extract(ANE).Methods Human oral mucosal FB were cultured in vitro,100 ?g/mL ANE was used as inducement and 10% DXKK drug serum as intervenor.The cell proliferation rates were measured by MTT assay,PCNA productions were examined by immunocytechemistry.Results MTT assay showed OD value in DXKK groups were significantly decreased compared with the induced group(P

Injury to the inferior vena cava (IVC) resulting from stab wound carries a high mortality rate. 1 Conventional open repair of the inferior vena cava is very challenging. Its morbidity and mortality ranges from 33-66%. 2 The predictors determining the outcome are the mechanism and type of injury, the initial blood pressure, the hemodynamic response to fluid resuscitation, the location of the vena caval injury, the presence of multiple other vascular and solid organ injuries.

Objective To review the clinical features and diagnosis of syphilitic aortic aneurysm .Methods A case of syphilitic aortic aneurysm was reported and the relevant literatures were reviewed .Results The patient showed progressive hoarse and heart murmurs and was diagnosed by computed tomographic angiography of aorta and syphilis serology test .In the era of antibiotics ,syphilitic aortic aneurysm is a very rare and dangerous case with covert onset ,various manifestations and high mortality .Conclusions The prevalence of late cardiovascular syphilis is increasing with the increase of early-onset syphilis and HIV epidemic .Syphilis serologic test should be available for the young patients with aortic aneurysm even though the risk factors of coronary heart disease are not present .Medical and surgical treatments for syphilis should be provided for the patients who are clinically suspected of syphilitic aortic aneurysm .

[Objective] To observe the effect of Leweiyin of traditional Chinese compounds prescription on gastrointestinal hormones and oxygen free radicals in IBS rabbits of spleen deficiency,and to explore the possible mechanism for the treatment of IBS with spleen deficiency.[Methods] 36 two-month-old rabbits with white feather and black eyes(WHBE rabbit),half male and half female,were randomly divided into 6 groups:normal group,model group,Xiangsha Liujun pill group,Lizhu Changle group,low-dose Leweiyin-treated group,high-dose Leweiyin-treated group.Except the normal group,others were made into IBS model of spleen deficiency which was induced by damp heat stress and gavage with Fanxieye as well as method of either hunger or gorge.The drug groups were administered preventively from the medium-term of the model reproduction to the end of modeling.The rabbits of each group were observed.NO,NOS,MDA,SOD in serum and SP in colonic membrane were detected.[Results] NO,NOS,MDA in serum and SP in colonic membrane significantly more increased than the normal group(P

Objective To study the effectiveness of postoperative radiotherapy and prognostic factors of soft tissue sarcoma. Methods Sixty-four soft tissue sarcoma patients admitted from June 1987 to May 1997 were treated by complete,partial or extended resection and postoperative external beam radiotherapy. Fourty-eight patients received 1.8～2.0?Gy/fraction,5 f/w, to a dose of 40～72?Gy (median 60?Gy). Sixteen patients received 1.2～1.5?Gy/f, bid, 6 hr interval to a dose of 64.8～85.0?Gy (median 66.5?Gy). The 5-year survival and locoregional control rates were estimated by Kaplan-Meier method. Logrank test and Cox regression were used to study the significance of predictive factors. Results The overall 5-year survival and locoregional control rates were 60.2%, 57.9% respectively. Those of the conventional group and hyperfractionated group were 54.3% and 61.9% vs 64.3% and 42.9% ,respectively. Cox regression analysis showed that histologic grade, size and site of the primary tumor, fractionation of radiotherapy were independent prognostic factors for survival. Conclusions Complete or extended resection followed by radiotherapy are satisfactory for soft tissue sarcoma. Histologic grade , size, site of the primary tumor and method of fractionation were prognostic factors.

0 05) The Kappa value was 0 83 and intraobserver agreement was bet ter b etween SeO 2 and SpO 2 .ConclusionThe sensor sticked to earlobe is feasible and simple to monitor puls oxygen saturation continuousl y in children