1.Molecular Identification and Distribution of Aquaporins in Human and Rat Testes.
Nam Cheol PARK ; Young Soo PARK ; Jin Sup JUNG
Korean Journal of Fertility and Sterility 2000;27(2):133-144
OBJECTIVE: Several water channels (aquapoins; AQP) that belong to the MIP (major intrinsic protein) family have identified. In the selected tissues including red blood cells or renal tubules, water movements are abundant and/or physiologically important. Unexpectedly, a high water permeability of human and ram sperm has been reported. Recent studies showed that AQP7 and AQP8 are present in testes so that the high water permeability of human sperm suggested to be mediated by AQPs. METHOD: To identify the identity of aquaporins expressed in testes, RT-PCR was performed using degenerative primers, which were designed to correspond to highly conserved sequences surrounding the Asn-Pro-Ala (NPA) motifs in the aquaporins. New expressed AQP series were reconfirmed by immunohistochemical study using rabbit polyclonal antibodies. RESULTS: DNA sequencing of PCR products revealed that AQP2 and AQP3 mRNA as well as AQP7 and AQP8 are expressed in human and rat testes, AQP2 are expressed in spermatozoa, interstitial cells and myofibroblasts and AQP3 are expressed in myofibroblasts of semineferous tubules on immunocytochemical stain. CONCLUSION: These results indicate that multiple aquaporins are expressed in testes, and that they may have important roles in the spermatogenesis and the germ cell function of testis.
Animals
;
Antibodies
;
Aquaporins*
;
Conserved Sequence
;
Erythrocytes
;
Germ Cells
;
Humans*
;
Myofibroblasts
;
Permeability
;
Polymerase Chain Reaction
;
Rats*
;
RNA, Messenger
;
Sequence Analysis, DNA
;
Spermatogenesis
;
Spermatozoa
;
Testis*
;
Water Movements
2.A procedure for delayed gastric emptying on pyloric preserving pancreaticoduodenectomy.
Tae Il JIN ; Jae Hong KIM ; Ju Sup PARK
Journal of the Korean Surgical Society 1993;45(5):588-695
No abstract available.
Gastric Emptying*
;
Pancreaticoduodenectomy*
3.A procedure for delayed gastric emptying on pyloric preserving pancreaticoduodenectomy.
Tae Il JIN ; Jae Hong KIM ; Ju Sup PARK
Journal of the Korean Surgical Society 1993;45(5):588-695
No abstract available.
Gastric Emptying*
;
Pancreaticoduodenectomy*
4.Clinical review of low anterior resection for rectal cancer using double stapling technique.
Byung Seok KIM ; Duk Jin MOON ; Ju Sup PARK
Journal of the Korean Society of Coloproctology 1993;9(2):143-150
No abstract available.
Rectal Neoplasms*
5.Clinical review of low anterior resection for rectal cancer using double stapling technique.
Byung Seok KIM ; Duk Jin MOON ; Ju Sup PARK
Journal of the Korean Society of Coloproctology 1993;9(2):143-150
No abstract available.
Rectal Neoplasms*
6.A clinical study on the by pass procedures of common bile duct for viliary disease.
Geon KIM ; Duk Jin MOON ; Ju Sup PARK
Journal of the Korean Surgical Society 1992;42(1):61-69
No abstract available.
Common Bile Duct*
7.Thiersch Operation for the Treatment of Old Patients with Rectal Prolapse.
Joon Cheol PARK ; Byung Seok KIM ; Duk Jin MOON ; Ju Sup PARK
Journal of the Korean Society of Coloproctology 1998;14(3):523-530
Although many kinds of operation for rectal prolapse exist, generally they could be divided into the transabdominal and transperineal approach. The former has low recurrence rate as compared with the latter, but needs laparotomy. Unfortunately, many patients with rectal prolapse are old and debilitated. So they are not suitable candidates for a major abdominal operation. For those patient, a transperineal method may be proper, but the recurrence rate may be high and continence is not always achieved. We reviewed 12 patients who were older than 70 years of age and treated by the Thiersch operation among the 52 cases of rectal prolapse from Feb. 1992 to Mar. 1997 at Kwangju Christian Hospital. The results were summarized as follows; 1) The male to female ratio was 1 : 1.4. 2) Durations of rectal prolapse were distributed from 6 months to 30 years. Seven cases had duration of longer than 10 years. 3) The preoperative incidence of incontience was 8 (67%) of 12 cases. Among the 8 patients with incontinence, the male was 3 (37.5%) and female was 5 (62.5%). 4) Postoperative review of incontinence revealed complete disappearance in 4 cases (50%), improvement in 3 cases (37.5%), and no improvement in 1 case (12.5%). 5) After the Thiersch operation, rectal prolapse recurred in only 1 of the 12 cases (8.3%), a wound infection developed in 1 case (8.3%), defecation difficulty due to the small Thiersch ring was present in 3 cases (25%). The results of this study show that the Thiersch operation may be an effective method in treating rectal prolapse, especially in patient who are old or have poor general condition and manifested incontinence.
Defecation
;
Female
;
Gwangju
;
Humans
;
Incidence
;
Laparotomy
;
Male
;
Rectal Prolapse*
;
Recurrence
;
Wound Infection
8.Tumor Angiogenesis and Vascular Endothelial Growth Factor Expression in Cervical Intraepithelial Neoplasia.
Hye Jean PARK ; Hye Jin PARK ; Hye Sung MOON ; Woon Sup HAN ; Sun Hee SUNG
Korean Journal of Pathology 2000;34(7):524-530
Angiogenesis is an essential requirement for development, progression, and metastasis of malignant tumors. Vascular endothelial growth factor (VEGF) is one of the important angiogenic factors. Recently the role of angiogenesis has been known in premalignant lesions. This study was performed to determine whether the angiogenesis and VEGF expression were increased in association with histological grade of cervical intraepithelial neoplasia (CIN) and to see the relationship between the angiogenesis and VEGF. Immunostainings for factor VIII and VEGF were performed on 52 cases of cervical neoplasia (12 cases of CIN I, 11 cases of CIN II, 15 cases of CIN III, 7 cases of microinvasive squamous cell carcinoma, and 7 cases of invasive carcinoma) and 5 cases of normal cervix. The results showed a significant increase of microvessel count from normal cervix through CIN grades to invasive squamous cell cacinoma. VEGF expression was increased in proportion to the CIN grades. There was no significant correlation between microvessel count and VEGF expression. In conclusion, the tumor angiogenesis is an early event in tumorigenesis of uterine cervix. In addition, no significant relationship between the microvessel count and VEGF expression in CIN suggests the possibility of other growth factors affecting mainly angiogenesis of premalignant lesion of uterine cervix.
Angiogenesis Inducing Agents
;
Carcinogenesis
;
Carcinoma, Squamous Cell
;
Cervical Intraepithelial Neoplasia*
;
Cervix Uteri
;
Factor VIII
;
Female
;
Intercellular Signaling Peptides and Proteins
;
Microvessels
;
Neoplasm Metastasis
;
Vascular Endothelial Growth Factor A*
9.Non-cirrhotic Portal Hypertension in Idiopathic Myelofibrosis: A case report.
Sung Eun KIM ; Young Nyun PARK ; Woo Ick YANG ; Jin Sup CHOI ; Chanil PARK
Korean Journal of Pathology 2000;34(5):386-388
We report a case of non-cirrhotic portal hypertension in a 73 year-old woman, who had 19-year history of idiopathic myelofibrosis. There were esophageal varix, splenomegaly, and ascites. The biopsied liver showed irregular sinusoidal/ perisinusoidal fibrosis and occasional central-to-central fibrous connection. In areas with extensive fibrosis, coarse collagen fibers filled the sinusoidal spaces and compressed hepatocytes. However, nodular regeneration was absent. Double immunohistochemical stain for smooth muscle actin and proliferation cell nuclear antigen (PCNA) revealed diffusely activated stellate cells, some of which showed nuclear PCNA staining. There was also extramedullary hematopoiesis with bizarre megakaryocytes. The portal vein and its branches were patent. Idiopathic myelofibrosis is a rare cause of non-cirrhotic portal hypertension: the portal hypertension was considered to be the result of sinusoidal/perisinusoidal fibrosis in this case.
Actins
;
Aged
;
Ascites
;
Collagen
;
Esophageal and Gastric Varices
;
Female
;
Fibrosis
;
Hematopoiesis, Extramedullary
;
Hepatocytes
;
Humans
;
Hypertension, Portal*
;
Liver
;
Megakaryocytes
;
Muscle, Smooth
;
Portal Vein
;
Primary Myelofibrosis*
;
Proliferating Cell Nuclear Antigen
;
Regeneration
;
Splenomegaly
10.Ultrasonographic ejection fraction of normal gallbladder
Jin Hun PARK ; Seung Yup KIM ; Yaung Hee PARK ; Ik Won KANG ; Jong Sup YOON
Journal of the Korean Radiological Society 1984;20(2):301-304
Real-time ultrasongraphy is a simple, accurate, noninvasive and potentially valuable means of studying gallbladder size and emptying. The authors calculated ultrasonographically the ejection fraction of 80 cases of normally functioning gallbladder on oral cholecystography, from June 1983 to April 1984, at the department of radiology, Hangang Sacred Heart Hospital. The results were obtained as follows; 1. Ultrasongraphic Ejection Fraction at 30 minutes after the fatty meal was 73.1+-16.85. 2. There was no significant difference in age and sex, statistically.
Cholecystography
;
Gallbladder
;
Heart
;
Meals