No abstract available.
Cholecystectomy

No abstract available.
Adrenalectomy*

To evaluate the ultrastructural changes and the mechanism causing liver injury by lead, light and electron microscopic(LM and EM) examination using Timm sulphide silver method(TSM) was done. Sprague-Dawley rats were divided into a control and 3 experimental groups. The experimental groups were orally administered 0.5% lead acetate(LA). Group 1 received a one time dose of 10 ml of LA by gastric intubation. Groups 2 and 3 continuously received LA instead of drinking water. The control group was composed of 3 rats in each group which did not receive any treatment. Rats of group 1, 2 and 3 and control were sacrificed at 1/2, 1, 1 1/2 hours, 2 days, and at 1, 2, 4, 6 and 8 weeks later, except group 3. Before sacrifice, they were perfused with 0.1% sodium sulphide and 2.5% glutaraldehyde through the abdominal aorta for TSM. The liver was taken for LM and EM examinations. Blood lead concentration began to increase from the 2nd day up to 3.29 microgram/ml at 2nd week, and the urinary delta-ALA level showed a steady increase from the 2nd day. LM and EM examination of liver revealed that absorbed lead granules in group 1 were transported into sinusoidal spaces, Kupffer cells, and the hepatocytes within 1 hour and then disappeared 1/2 hour thereafter. In group 2 deposited lead was found in the hepatocytic cytosol bound to mitochondria. That in turn inhibited mitochondrial respiration with resultant mitochondrial swelling at the 1st week and thereafter at 6th week myelin figure formation and condensation of mitochondria, and peroxisomes were increased at 8th week. Based on these results it can be concluded that a transient intake of subletal dose of LA is biotransformed completely by periportal hepatocytes within 1 1/2 hours, but excessively accumulated lead can induce liver cell injury due to lipid peroxidation of membrane by direct toxic effect of lead and by products of lipid peroxidation. We postulate that lead acetate triggers presumably primarily mitochondrial membrane injury and then other organellar changes may play a role in disturbance of a network of interacting of key events capable of causing cell death.
Rats ; Animals

Nineteen cases of leiomyoma of the skin were examined clinicopathologically. This group included 12 cases of angioleiomyoma, 5 cases of solitary piloleiomyoma, and 2 cases of multiple piloleiomyomas. 1) All twelve angioleiomyomas occured as solitary lesion in the extremities. There was a preponderance in females with a ratio of 2:1. The ages of patients ranged from 24 to 80 years and only one was below the age of 30 years. Six tumors were either painful or tender. Nine tumors in subcutaneous fat were shelled out at surgery. All tumors did not exceed 4cm in diameter. Histologically they could be separated into ten cases of the solid type and two cases of venous type according to Morimoto's classification. Although actin or desmin was easily detected in all tumors, the diagnosis was better made using a combination of hematoxylin-eosin and Masson trichrome stains. 2) Five cases of solitary piloleiomyoma were slow-growing intradermal nodules. The ages of patients ranged from 10 to 77 years. All five cases were female. The lesions were located on the extremities, back and sholuder. Pain or tenderness was present in 3 cases among these tumors. Histologically, all tumors were characterized by subtle poorly circumscribed proliferation of benign smooth muscle in the dermis. 3) Two multiple piloleiomyomas from two female patients, aged 50 and 40 years, were situated on the shoulder and thigh, respectively. Pain was induced by change of temperature in the shoulder lesion. Histologically they were identical to the solitary piloleiomyoma.
Female ; Humans

As an optimal quality of the restorations, there should be a least amount of seating discrepancy between the casting and abutment teeth. However, high viscosity of the cementing medium and its resulting thickness ay prevent complete seating of the restoration. The use of die spacing material provides adequate internal relief for the cementing medium. The purpose of this study is to compare the thickness of three commonly used die spacing materials. Materials and Methods : Stone plates were fabricated and divided into 12 sections to be painted with die spacers. Tru-Fit, Whip-Mix and Belle de St. Claire die spacer which are commonly used in dental practice were tested in this study. Each die spacers were painted layer by layer according to the manufacturer's recommendation. The average thickness of each die spacers were measured with light microscope(x100) and compared between them. Results and Conclusions: A silver-colored Tru-Fit die spacer has the lowest value of thickness without statistical significance comparing with a gold-colored Tru-Fit die spacer and a gray layer of Whip-Mix die spacer has the highest value of thickness without any statistical significance comparing with Belle de St. Claire die spacer. Three and four layers of Tru-Fit die spacer and two layers of Whip-Mix and Belle de St. Claire die spacers seem to be in the acceptable range of thickness of 25 to 45micrometer for optimal seating of the restorations. The standard experimental design and method should be further evaluated for more consistent and objective results.
Paint ; Research Design ; Tooth ; Viscosity

No abstract available.
Hearing Loss, Sensorineural* ; Stellate Ganglion*

No abstract available.
Bias (Epidemiology)* ; Case-Control Studies* ; Humans ; Triplets*

PURPOSE: Although most researchers agree that platelet-rich plasma (PRP) is a good source of autogenous growth factors, its effect on bone regeneration is still controversial. The purpose of this study was to evaluate whether increasing angiogenic factors in the human PRP to enhance new bone formation through rapid angiogenesis. MATERIAL AND METHODS: In vitro, the human platelets were activated with application of shear stress, 20 microgram/ml collagen, 2 mM CaCl2 and 10U thrombin/1 x 109 platelets. Level of vascular endothelial growth factor (VEGF) and platelet microparticle (PMP) in the activated platelets were checked. In the animal study, human angiogenic factors-enriched PRP was tested in 28 athymic rat's cranial critical bone defects with beta-TCP. Angiogenesis and osteogenesis were evaluated by laser Doppler perfusion imaging, histology, dual energy X-ray densinometry, and micro-computed tomography. RESULTS: In vitro, this human angiogenic factors-enriched PRP resulted in better cellular proliferation and osteogenic differentiation. In vivo, increasing angiogenic potential of the PRP showed significantly higher blood perfusion around the defect and enhanced new bone formation around acellular bone graft material. CONCLUSION: Angiogenic factor-enriched PRP leads to faster and more extensive new bone formation in the critical size bone defect. The results implicate that rapid angiogenesis in the initial healing period by PRP could be supposed as a way to overcome short term effect of the rapid angiogenesis.
Angiogenesis Inducing Agents ; Animals ; Blood Platelets ; Bone Regeneration ; Calcium Phosphates ; Cell Proliferation ; Collagen ; Durapatite ; Humans ; Intercellular Signaling Peptides and Proteins ; Osteogenesis ; Perfusion ; Perfusion Imaging ; Platelet-Rich Plasma ; Rats, Nude ; Transplants ; Vascular Endothelial Growth Factor A

No abstract available.
Dental Porcelain* ; Hardness* ; Resin Cements*

No abstract available.
Pregnancy*