Objective To analyse the health system responsiveness of Huzhou of Zhejiang Province. Methods Performance evaluation method proposed by WHO was employed for the study of responsiveness. The local residents of Huzhou of Zhejiang Province in 2007 were investigated with multilevel cluster stratified sampling. Data were obtained by household survey and involved items such as dignity,independence,privacy,communication,timely attention,equipment,social support and selection of medical staff,and were subjected to empirical analysis. Results Household survey was conducted in 1353 residents,among whom 28.16% lived in town and 71.84% lived in countryside. The index of health system responsiveness of Huzhou was 7.96,slightly higher than that of Zhejiang Province (7.71). The responsiveness was significantly higher for countryside than that for town in independence,communication and timely attention (P

Objective: To investigate the effect of mannitol combined with the vein graft supported with bone marrow mesenchymal stem cell (BMSC) on the cognitive function and the expression of brain-derived neurotrophic factor (BDNF) in brain tissue of vascular dementia (VD) rats. Methods: The BMSCs of rat were isolated and purified by whole bone marrow culture and adherence screening methods. Seventy nine healthy male SD rats were randomly assigned to sham operation group (n = 10) and model (n = 69) groups. A VD model was established by the ligation of the bilateral common carotid arteries at an interval of 3 days. Four weeks after modeling, the 32 rats in the model group were randomly allocated into mannitol pretreatment BMSC transplantation group (tail intravenous injection of mannitol 1.5 g/kg, tail intravenous injection of 1 × 106 mL BMSCs 1 mL after pretreatment for 1 to 30 minutes), BMSC transplantation group (injecting the same amount of BMSCs but not mannitol), and medium control group (injecting the same amount of basal medium). After removing the dead rats, there were 9, 11, and 8 rats, respectively in the above 3 groups. Seven rats were included in the sham operation group. Morris water maze test was used to detect cognitive function of the rats, and an enzyme-linked immunosorbent assay was used to detect the BDNF content in brain tissue 4 weeks after intervention. Results: Circled Digit oneComparing the 4 groups at the same time points, the escape latency in the BMSC transplantation group was shorter than that in the medium control group (all P < 0.01), and residence time in the platform quadrant was prolonged (P < 0. 05). The BMSC transplantation group after mannitol pretreatment was also significantly shorter than the escape latency in the BMSC transplantation group (P < 0. 01), the residence time in the platform quadrant was prolonged (P < 0.05), and they were all close to the level of the sham operation group (P > 0.05). Circled Digit twoThe BDNF content in the BMSC transplantation group was significantly higher than that in the medium control group in the frontal cortical and hippocampal regions (P < 0.01). The mannitol pretreatment BMSC transplantation group was more significantly higher than the BMSC group (P < 0.01), however, they were lower than the level in the sham operation group (P < 0.01). Conclusion: Compared with intravenous injection of BMSCs alone, the improvement of cognitive function of the VD rat model treated with mannitol combined with BMSC transplantation is much better; the BDNF expression in cortex and hippocampus is higher.

Objective To construct an independent, comprehensive, analyzable and applicable data source using existing technique and massive data in the hospital for fulfilling manager's impersonality quantity management and appraised needs on working quality, efficiencies and benefit. MethodsAn integrated ODS database was built up by analyzing ODS running mode, designing data model, building fact tables and dimension tables, putting forward a project of data transfer. ResultsAfter solving disperse applying of transaction processing, data conflietion of multi-application and off-line of history data, a new ODS database can be constructed to provide an active, objective data source for manager. Conclusion The ODS database based on HIS can make great data in hospital used fast and efficiently in clinician scientifie management and decision.

Objective To study whether solasodine hydrochloride (SBHL) could enhance the effect of arsenic trioxide in inducing apoptosis and affecting telomerase activity in cervical cancer HeLa cells. Methods Using cell culture methods, cervical cancer HeLa cells were cultured in vitro. The optimal concentration of SBHL was determined by MTT method from 0, 10, 20, 40, 80, 160, to 320 μmol/L. HeLa cells were grown in improved RPMI1640 supplemented respectively with arsenic trioxide(5 μmol/L As2O3), As2O3(5 μmol/L)+ SBHL( 40 μmol/L) and none (control group). The growth morphology of HeLa cells was observed under phase contrast microscopy after culture for 24, 48, and 72 h. Apoptosis of HeLa cells was determined under transmission electronic microscopy. The method of MTT was used to study the cell survival percentage. The technique of flow cytometry was used to measure cell cycle and cell apoptosis percentage. The method of tartrate-resistant acid phosphatase-enzyme linked immunosorbent assay (TRAP-ELISA) was used to determine telomerase activity of HeLa cells. Results Under phase contrast microscopy, in control group HeLa cells were round, densely packed; in As2O3 group the numbers of the cells were less, cell spacing increased; in As2O3 + SBHL group the cells shrinked significantly, nuclear fragmented as a petal-like, gap became larger. Under transmission electronic microscopy, there were rich microvillus on the cell surface in control group, cell intervals clear, immature connections, and the intervals did not close. The structure of the mitochondria in the cytoplasm was integrated. Most of the chromatin in the nucleus were, euchromatin and characteristics of apoptosis with heterochromatin increased and the chromatin condensed into masses, on the boundary of nuclear membrane. The microvillud on the cell surface were ruptured and decreased in As2O3 + SBHL group. The chromatin condensed into masses. The formation of apoptotic bodies was observed. The difference was statistically significant between groups in cell survival percentage at 24, 48, 72h(x2 = 10.39 , 13.88 , 17.21,respectively, all P < 0.05). Cell survival percentage in SBHL + As2O3 group (52.80%) was significantly less than that of As2O3 group(77.51%, x2 = 9.29, P < 0.05) at 72 h. In cell cycles, the difference was statistically significant between groups in C1 phase and S phase(F = 7.46,22.14, all P < 0.05), respectively. Compared with , control group[ (41.57 ± 1.56)%, (50.45 ± 2.37)%], cell percentages in S phase in As2O3 + SBHL group[(20.06 ± 4.98)%] and As2O3 group[(27.10 ± 5.32)%] were decreased(P< 0.05 or < 0.01), while cell percentage in C1 phase was increased[(58.70 ± 5.18)%, (69.67 ± 4.17)%, P< 0.05 or < 0.01]. The difference was statistically significant between groups in apoptotic percentage of HeLa cells (F = 4.01, P < 0.05). Compared with control group[ (1.18 ± 1.40)%], apoptosis percentage was significantly increased in As2O3 + SBHL group and As2O3 group [(21.08± 1.22)%, (6.04±2.53)%, P< 0.05 or < 0.01], respectively, and As2O3 + SBHL group was higher than As2O3 group(P < 0.01). The difference was statistically significant between groups in telomerase activity (F = 21.28, P< 0.05). Telomerase activity was inhibited in As2O3 group(1.214 ± 0.621) and As2O3A + SBHL group(0.865 ± 0.284) compared to control group (2.107 ± 0.057, all P < 0.05), and telomerase activity in As2O3 + SBHL group was lower than that of As2O3 group (P < 0.05). Conclusions SBHL enhances the effect of As2O3 in inducing apoptosis in HeLa cells, which is related to its inhibiting telomerase activity in HeLa cells.

OBJECTIVE:To study the antioxidation activity of different processed products of polygonum multiflorum thunb and its crude.METHODS:The capabilities of different processed products of polygonum multiflorum thunb and its crude in eliminating free radical were studied and compared by chemical luminescent technique.RESULTS:The luminescent inhibi?tion ratio was used to represent the activity of superoxide dismutase(SOD),the result showed that the ratio of luminescent in?hibition of the crude sample of polygonum multiflorum thunb ranked the top,i.e.the crud sample had the highest antioxidation activity,then the black bean product,product that steamed in clear soup and that steamed in black bean alcohol solu?tion.CONCLUSION:If one can develop a preparation method in which only the purgative component of polygonum multiflo?rum thunb were destroyed while the effective components remained,the tonification function of which can be enhanced and the anti-aging effect of which can be brought into full play.

Air ; analysis ; Chromatography, Gas ; methods ; Phenylphosphonothioic Acid, 2-Ethyl 2-(4-Nitrophenyl) Ester ; analysis ; Workplace

Air Pollutants, Occupational ; analysis ; Chromatography, Gas ; methods ; Workplace ; alpha-Chlorohydrin ; analogs & derivatives ; analysis

Asthma ; blood ; Child ; Child, Preschool ; Complement Membrane Attack Complex ; analysis ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Intercellular Adhesion Molecule-1 ; blood ; Male ; Vascular Cell Adhesion Molecule-1 ; blood

This pap er introduces something of photodynamic therapy including the basic principles,pho tosensitizers and recent situation of clinical trial.Its light source,research and clinical applications to malignant tumors are also presented.

Objective To investigate the genetic diversity of five populations of cultivated Morinda officinalis in Guangdong Province using random amplified polymorphic DNA(RAPD).Methods Sixty-four individuals in five populations of cultivated M.officinalis were analyzed by RAPD markers to determine the genetic variations among the populations.The data of genetic diversity were analyzed with Popgen32 software.Results The levels of genetic variation and patterns of population structure in M.officinalis were investigated using RAPD markers.Of the 100 primers screened,15 primers produced highly reproducible RAPD bands,using these primers,224 discernible DNA fragments were generated with 112(50.00%) polymorphic fragments,indicating considerable genetic variation at the species level.In contrast,there were relatively high levels of polymorphism at the population level with the percentage of polymorphic bands ranging from 37.05% to 53.13%,and the mean percentage of polymorphic loic(P=45.86%).Genetic variation among populations was detected based on Nei's genetic diversity analysis(0.175 6),Shannon's diversity index(0.287 6) for every population with Shannon's index 0.103 3—0.236 2 and Nei's indexes 0.074 5—0.154 0.Conclusion There is a little genetic differentiation among populations of cultivated M.officinalis and result in different cultivated types,the genetic diversity with M.officinalis(POP2) is higher than that of other populations.It may be the main reason of the difference of cultivated M.officinalis quality.