Objective To investigate the antineoplastic action of 5-aza-2-deoxycytidine(CdR) in combination with arsenic trioxide on HL 60 myeloid leukemia cells.Methods HL 60 leukemia cells were incubated with CdR and arsenic trioxide and their antineoplastic action was determined by cells grouth curve,surface differentiation antigen spikes of subdiploid of cell cycle and superoxide dismutase etc.Results CdR combined with arsenic trioxide produced a greater growth inhibition andmove strong induced the differentiation of HL 60 cell than that in anyone of the two agents used independently.Conclution These results suggest that the arsenic trioxide may enhance the anti-leukemic action of CdR.

Objective To investigate the effects of cinnamic acid to enhance the exercise endurance in mice .Methods Through the exhaustive swimming to establish mice exhaustive exercise model ,the mice were divided into control group ,exercise control group and exercise cinnamic group(n=10) ,the mice exhaustive swimming time were comparted ,blood lactic acid(LAC) lev‐els were used to explore the endurance of cinnamic acid in mice;the super oxide dismutase (SOD) ,malondialdehyde (MDA) and peroxidase (CAT) content in liver homogenates were used to detect mice endurance enhancement and antioxidant capacity of cin‐namic acid ,and expression of Na+ /K+‐ATP enzyme ,Ca2+ /Mg2+‐ATP enzyme on mice quadriceps by Western blot to further study its effect on improving exercise capacity in mice .Results Cinnamic acid significantly prolonged the swimming time of mice;Exercise cinnamic group compared with control group:although the LAC content in plasma elevated ,the difference was not statistically sig‐nificant(P>0 .05) ,SOD and CAT in liver homogenate was significantly decreased(P< 0 .05) ,MDA increased significantly(P<0 .01) ,CAT was decreased but with no statistically significant(P>0 .05) ,the expression level of Na+ /K+‐ATP enzyme and Ca2+ /Mg2+‐ATP enzyme decreased significantly (P< 0 .01) .Exercise control group compared with control group:the LAC content in plasma increased significantly(P<0 .01) ,SOD and CAT in liver homogenate was significantly decreased(P<0 .01) ,MDA increased significantly(P< 0 .01) ,expression level of Na+ /K+‐ATP enzyme and Ca2+ /enzyme Mg2+‐ATP decreased significantly (P<0 .01);Exercise cinnamic group exercise control group:the LAC content in plasma was significantly reduced(P<0 .01) ,SOD and CAT in liver tissue significantly increased(P< 0 .01) ,MDA decreased significantly(P<0 .01) ,CAT decreased significantly(P<0 .01) ,the expression level of Na+ /K+‐ATP enzyme and Ca2+ /Mg2+‐ATP enzyme decreased significantly(P<0 .05) .Conclusion Cinnamic acid has good oxidation resistances and anti‐fatigue ability .

Objective To assess the cerebral protective effects of different doses of propofol during mild hypothermic cardiopulmonary bypass (32℃-35℃) .Methods Thirty consecutive ASA Ⅱ-Ⅲ patients aged 18-50 years, undergoing elective cardiac surgery under mild hypothermic CPB were randomly divided into 3 groups: in group Ⅰ and Ⅱ the patients were given a bolus of propofol 1 mg kg-1 at the beginning of CPB followed by propofol infusion at a rate of 3 mg ? kg -1? h -1 (group Ⅰ ) or 6 mg? kg-1 ?h-1 (group D ) till the end of CPB and in group Ⅲthe patients received midazolam infusion at 0.2 mg? kg-1 h-1 instead of propofol. Anesthesia was induced with intravenous midazolam 0.15 mgkg-1 ,fentanyl 5 ?g kg-1 and vecuronium 0.1 mg? kg-1 .After tracheal intubation fentanyl 30-50 ?g kg-1 was infused during CPB and muscle relaxation was maintained with intermittent iv boluses of vecuronium. Surgery was performed with mild hypothermic CPB(32℃-35℃ ) .Arterial blood pH and PaCO2 were maintained within normal range and MAP was maintained at 50-80 mm Hg during CPB. Hematocrit was maintained at 25%-30% .Internal jugular vein(IJV) was cannulated and the catheter was advanced retrogradely till jugular bulb. Naso-pharyngeal temperature was monitored. Blood samples were taken simultaneously from artery and UV befor CPB (A) ,when mild hypothermia was being stably maintained(B) ,when the patients were rewarmed to 361 (C)and 30min(D),4-6 h(E)and 24 h(F) after discontinuation of CPB, for determination of cerebral oxygen metabolism and balance, cerebral lactate production (CLP), blood malondialdehyde (MDA) and superoxide dismutase (SOD) levels. CLP was calculated from arterial-cerebral venous blood lactate difference /arterial blood lactate concentration. Lactate oxygen index (LOI) was calculated from arterial-cerebral venous blood lactate difference /arterial-cerebral venous blood O2 content difference.Results In group I (low propofol dose) 30% patients developed cerebral O2 inbalance during rewarming but blood MDA, CLP and LOI were decreased. In midazolam group (group Ⅲ) cerebral O2 balance was improved but there was no depression of blood MDA, CLP and LOI. In groupⅡ (high propofol dose) cerebral oxygenation was improved and blood MDA level, CLP and LOI were significantly decreased.Conclusion The study shows that propofol infusion at a rate of 6 mg?kg-1? h-1 during CPB can improve cerebral oxygenaton, inhibit blood MDA level, CLP and LOI, and plays a role in the cerebral protection during cardiac surgery with CPB, but more vasoconstrictors are needed.

No abstract available.
Humans* ; Melanocytes* ; Tretinoin*

Angiolymphoid hyperplasia with eosinophilia (ALHE) is a benign, uncommon disorder of unknown etiology, that usunlly appears as papules or nodules on the head and neck. Histopathologically, ALHE is a angioproliferating lesion which shows characteristically plump epithelioid or histiocytoid endothelial cells, accompanied by an inflammatory infiltrate that mainly consists of lymphocytes and eosinophils. We report a case of angiolymphoid hyperplasia with eosinophila associated with arteriovenous malformations in a 23-year-old man. In our patient, we observed arteriovenous malformation, changes which could have occurred by vascular repair due to a vascular malformation.
Angiolymphoid Hyperplasia with Eosinophilia ; Arteriovenous Malformations ; Endothelial Cells ; Eosinophils ; Head ; Humans ; Hyperplasia* ; Lymphocytes ; Neck ; Vascular Malformations ; Young Adult

Child ; Child Health Services ; trends ; Delivery of Health Care ; organization & administration ; Education, Medical ; Hospitals, Pediatric ; economics ; organization & administration ; Humans ; Pediatrics ; trends ; Specialization ; Urban Population

From June 2004 to December 2005 anesthesia was done for 43 patients undergoing orthotopic heart transplantation (OHT) in Fuwai Hospital. Most patients were premedicated with oral diazepam 5-10 mg, and intramuscular morphine 0.2 mg?kg-1 and scopolamine 0.1-0.3 mg. Radial artery was cannulated and Swan-Ganz catheter was placed. ECG, direct BP, HR, CVP, PAP, CO, SpO2, SvO2, PETCO2 were monitored before and during operation. Anesthesia was induced with midazolam 1-3 mg, etomidate 0.2-0.3 mg?kg-1 , fentanyl 5-15?g?kg-1 or sufentanil 50-100?g, vecuronium 0.1 mg?kg-1 or rocuronium 0.6 mg?kg-1 , and maintained with isoflurane inhalation and propofol infusion and intermittent i. v. boluses of fentanyl or sufentanil and vecuronium. Hemodynamic suppression was mild during anesthesia. The average duration of aortic cross-clamping and CPB was 57-133 min and 123-230 min. The cardiovascular support used for weaning the patients from CPB included dopamine, ephedrine and isoproterenol. Nitroglycerin, NO and iloprost were administered for pulmonary artery vasodilation. Pacing was started at the termination of CPB because of bradycardia in 1 of 43 patients. Sinus rhythm appeared after the patients were weaned from CPB and the function of the transplanted heart was satisfactory. Basiliximab, cyclosporine A, cellcept and methyl prednisolone were administered for immunosuppression during perioperative period. Forty-two of the 43 patients were discharged from hospital without any rejection episodes or other complications. One patient died of multiple organ failure after heart-kidney transplantation.

Objective It was estimated that about 70% patients suffered from mild brain function disturbances after cardiac surgery with CPB Methods for early detection of brain injury after CPB in current use like EEG, transcranial Doppler, CT, MRI are expensive and not sensitive It was repored that combined assays of S 100 protein and NSE were conducive to early detection of brain ischemic injury and prediction of the prognosis The purpose of this study was to assess the changes in cerebral blood S 100 protein and NSE levels during and after open heart surgery with mild hypothermic CPB Methods 15 consecutive ASA Ⅱ Ⅲ patients undergoing elective open heart surgery under CPB were studied Aortic cross clamping time was 30 60 min and CPB time was less than 120 min Patients with hypertension and neurologic or endocrine diseases were excluded Anesthesia was induced with midazolam 0 15mg/kg, fentanyl 5?g/kg and vecuronium 0 1mg/kg Fentanyl 30 50 ?g/kg was continuously infused after tracheal intubation and during CPB Vecuronium was given intermittently to maintain muscle relaxation Midazolam was infused at 0 2mg?kg -1 h -1 during CPB Temperature was reduced to 32℃ 35℃ during CPB Aterial blood pH and PCO 2 were maintained within normal range and Hct between 25% 30% during CPB Internal jugular vein was caunulated and the catheter was advanced retrogradely until jugular bulb Jugular venous blood samples were taken for determination of S 100 protein and NSE content before CPB (A),when mild hypothermia (32℃ 35℃ ) was steadily maintained (B), rewarming to 36℃ (C), 30 min (D),4 6h (E) and 24h (F) after termination of CPB Results (1) After institution of CPB, S 100 protein level increased significantly (P

Objective To evaluate the efficacy and safety of 0.75% ropivacaine versus 0.75% bupivicaine for combined spinal and epidural anesthesia(CSEA) for cesarean section. Methods Forty primiparae(ASA Ⅰ-Ⅱ) scheduled for elective cesarean section were divided into two groups: ropivacaine group received 0.75% ropivacaine 1 .2-1 . 4ml(9-10. 5mg) for spinal anesthesia and bupivicaine group received same amount of 0.75% bupivicaine. During operation when spinal analgesia was inadequate, 1.6% lidocaine was supplemented via epidural catheter. Blood pressure , heart rate and SpO2 were monitored. Sensory block(pin prick), motor block(modified Bromage scale), quality of analgesia and relaxation of abdominal wall were assessed. Apgar score of the neonates and umbilical artery blood gas as well as side effects were recorded. Results There was no significant difference between the two groups in age, height, body weight of the patients and duration of operation. The height of block was comparable between the two groups but the onset time was longer and duration of block was shorter in ropivacaine group. Analgesia and muscle relaxation were satisfactory and Apger score was 10 at 1 mm and 5mm in both groups. Blood gas values were within normal range in both groups. Motor block was weaker with ropivacaine than that with bupivacaine(P

Objective To investigate the mechanism for rhein to improve the insulin sensitivity in diabetes mellitus. Methods Male Wistar rats were randomly divided into normal control group (NC,n=15) and diabetic group (DM,n=40) induced by STZ and high-fat diet.Diabetic rats were randomly divided into diabetic control group (DM-C,n=15) and diabetic group treated with rhein (DM-T,n=15).Then DM-T group was given intragastric administration with rhein at the dose of 100 mg/ (kg-1?d-1) for 11 weeks.FBG,fasting serum insulin (FIns),triglyceride (TG),total cholesterol (TC),insulin sensitivity index (ISI) and other biochemical parameters in each group were measured.The protein expressions of PPAR-? and GluT-4 were determined by immunohistochemistry and Western blot. Results At the end of the 17th week,compared with NC group,DM-C group showed higher FBG and TG,lower ISI,lower protein expressions of PPAR-? and GluT-4 in the adipose tissue.Compared with DM-C group,DM-T group showed lower FBG [(15.73?3.08 vs 22.57?3.23) mmol/l,P