Next generation sequencing technologies,providing high speed and throughput that contribute greatly to DNA sequencing,have many applications in clinical virology,including identification of novel or unsuspected viruses,evaluation of human viromes,investigation of viral genetic diversity and evolution,monitoring of antiviral drug resistance,and analysis of virus-host interaction.Meanwhile,due to the complexity in bioinformatics data processing and lack of quality management standards,further efforts and explorations need to be put in the application and quality management of NGS in clinical laboratory.

Objective To evaluate the effect of combination of phacoemulsification and trabeculectomy on patients with cataract and glaucoma.Methods 19 patients(23 eyes)with co-existing glaucoma and cataract were treated with triple procedure by means of scleral tunnel flap.The mean follow-up period was(15.8 ±3.5)months.Results Compared with preoperative visual acuity,16(64%)of 25 eyes had a best corrected visual acuity of ≥0.6 at the last follow-up(0.1～1.0)(P＜0.01);The mean preoperative intraocular pressure(IOP)was(23.01 ±2.63)mmHg which decreased to(14.02 ±2.05)mmHg(P＜0.05 ,P ＜0.01)after treatment.Conclusion The triple procedure is effective for treating patients with co-existing cataract and glaucoma,which could enhance the visual acuity,control IOP and reduce complications.

An animal model of hemorrhagic shock is widely applied .It is an important platform to clarify the pathogenic mechanism, develop hemostasis materials , test first aid equipments and filter resuscitation medications .This paper summa-rizes the commonly used animal models of hemorrhagic shock at home and abroad , focusing on animal selection , model cre-ation, influencing factors , and fields of application in order to provide useful reference for model selection and application by medical practitioners and researchers .

Objective To explore effect of small dose of diuretics combined with Dahuangzhechong pill on testosterone, Cystatin C(CysC), leptin and β2 microglobulin(β2-MG) in serum in patients with chronic renal failure.Methods 69 cases of kidney disease patients were collected and divided into two groups.Testosterone, Cystatin C, leptin and β2 microglobulin in serum were compared before and after the treatment.The control group was given hydrochlorothiazide tablets, treatment group was given Dahuangzhechong pill based on the control group.All patients were treated for 3 months. Results After treatment, levels of testosterone elevated (P <0.05), CysC, leptin and β2 microglobulin decreased (P <0.05) in the two groups compared with before treatment;Compared with the control group , level of testosterone was higher (P<0.05), levels of CysC ,leptin and β2-MG were lower (P<0.05).Conclusion Low dose diuretics combined with Dahuang Zhechong pill has good effect in treatment of chronic renal failure curative.The mechanism may be related to elevate serum testosterone levels and reduce serum CysC , leptin and β2-MG level in patients.

ObjectiveTo analyze the results of implementing the tutorial system for medical interns, and to put forward some suggestions. MethodsThe medical interns and the doetors selected as tutors were asked to do questionnaires to study the tutorial system's feasibility and effectiveness. Simultaneously, we adopted the Objective Structured Clinical Examination ( OSCE ) to assess its process and resuits. Results74.3％ of the students and 75.5％ of the doctors think that they need the tutorial system,73.2％of the students think that this system can train better clinical skills,67.9％ of the doetors think that they can review the knowledge learned through teaching. Implemented after one year, students' OSCE scores are improved. ConclusionTutorial system for medical interns has achieved preliminary success, and the preparatory work and related-system building need to be further improved.

Referring to relative documents,it reviews the developments on the relation between IIS and gastro-intestinal hormones(gastrin,gallbladder bile-syst contractin,active vessel intestinal peptide and article P),and sums up the relativity study on the relativity between different TCM types of IIS and gastro-intestinal hormones secretion level.

Diagnostics is the bridge from basic medicine to clinical medicine.For the students contacting the clinic for the first time,we take measures to cultivate the consciousness of showing love to patients and dedicating yourself to work.It is beneficial to cultivate the students'ability in clinical practice.

Cognitive impairment caused by ischemic stroke is becoming more and more concerned.It is crucial to explore its pathophysiological mechanism and related clinical predictive indexes for understanding the occurrence,development and treatment of the disease.This article discusses the pathophysiologic mechanism of cognitive impairment after stroke from the aspects of white matter damage,amyloid abnormal deposition,blood-brain barrier damage,and synaptic plasticity injury,and reviews the risk factors,imaging and biological markers that can be used to predict cognitive impairment after stroke.

Objective To study the status quo and tendency of the utilization of antibiotics in our hospital in order to provide ref-erence for clinical rational drug use .Methods A retrospective analysis was performed on the consumption sum ,DDDs and defined daily cost(DDC) of antibiotics ,which taken from HIS system in drug storehouse from 2010 to 2013 .Results 2010~2013 ,the anti-microbial drug sales in proportion to the amount of drug sales were 35 .99% ,26 .47% ,14 .69% and 14 .52% respectively .Cephalo-sporins andβ-lactamase inhibitor have been in the forefront of the antimicrobial agents in the sales amount .The sales amount of Ce-foxitin sodium and Piperacillin/tazobactam has rapid grow th ,for three consecutive years of antimicrobial agents in the sales amount ordering frist and second .2010~2013 ,Cephalosporins and Macrolides antibiotics has been in the forefront on the sorting of DDDs . The DDDs of antifungal drugs increased year by year ,quinolones and nitroimidazoles was declining .The DDDs of Clarithromycin tablets ,Azithromycin enteric-coated capsules and Roxithromycin Capsules in the front row .2011-2013 ,the DDC of Cefuroxime in-creased the larger from 15 .37 to 68 .06 .Conclusion The antibacterial drug sales amount of drug sales amount is reduced year by year from 2010 to 2013 ,and the application of antimicrobial agents is given priority to Cephalosporins ,Antifungal drug sustained growth ,Macrolides antibiotics and Penicillins keep at a high level .

A total of 587 keywords expressing the health information needs of diabetics were selected from diabetes-related 8762 questions covered in Yahoo Q and A, coded according to the cataloguing system and classification strategies of diabetes information, processed by text formatting, and clustered by multi-dimensional analysis, which showed that the most concerned health information in diabetics is the daily management, diagnosis and treatment of diabetes while rare attention is paid to its prevention.