Thirty-six aborted embryos ranging from 64 days to 8 months were collected and epiphysial cartilage of the finger and long bones of limbs were studied histologically. The results are as follows: 1.Degeneration of cartilage cells of different degrees were seen in the cartilaginous tissues examined. 2.Some of the cartilage cells necrosis were alone and others formed a mass of 3 to 5 or from 5 to 10 cells. 3.The elder the age of embryo was, the higher the rate of cell necrosis would be. 4.There were noncellular regions and giant cells in the normal cartilaginous tissue. The authors consider these changes are rather physiological in nature and would be a necessary process in the development of the cartilage.

Objective To investigate the effect of Shuxuetong injection on anticoagulation of nephrotic syndrome.Methods 42 patients were randomly divided into 2 groups:Shuxuetong group and control group.The Shuxuetong group were injected Shuxuetong injection while the control group were injected low molecular dextran and Danshen injection.Hemorheology and clotting index were detected before and after treatment.Results Hemorheology and clotting index of the two groups were improved.Hemorheology and clotting index of the Shuxuetong group were improved better than that of the control group.Conclusions The results suggested that the Shuxuetong injection was a safe and effective anticoagulation drug for the treatment of nephrotic syndrome.

Enzyme histochemistry of neurons in the enteric nerve plexus of guinea pigs were studied with light microscope semi-quantitatively and microphotometer quantitatively. The results showed that the neurons differ greatly in A1P (alkaline phosphatase), AcP (acid phosphatase), 5'-Nase (5'-Nucleotidase), TPPase (thiamine pyrophosphatase), NsE (non specific esterase) and ChAT (choline acetyltransferase). There were disparities to a certain extent in reactions of MAO (monoamine oxidase), AP (aminopeptidase) and AChE (acetylcholinesterase) among different segments of gastrointestinal between submucous and myenteric plexus, but all neurons were positive for the enzymes stated above. The neurons in each ganglion were relatively similar in the enzyme activities. There were about 50-66% neurons in the enteric nerve plexus showing strong reaction of ChAT, which may be cholinergic neurons. There were significant differences in enzymatic activities, except NsE, between submucous plexus and myenteric plexus statistically. Submucous plexus showed stronger reactions of AcP and AP than those of myenteric plexus, while myenteric plexus showed stronger reactions of A1P, 5'-Nase, TPPase, MAO, ChAT than those of submucous plexus. The ganglia of intramural plexus in stomach were not well developed as those of intestine, especially the submucous plexus of stomach, in which there were only few scattered neurons, and they showed weaker enzyme activities than those of intestine. The enteric neurons in duodenum and proximal colon showed strongest activities for most enzymes among different segments of intestine. The above results indicate that the enteric neurons exist remarkable differences in metabolism and functional states.

Objective To evaluate the feasibility and influence factors of ultrasound guided thrombin injection(UGTI) for the treatment of iatrogenic femoral pseudoaneurysms(PA). Methods Fifteen iatrogenic PA patients following femoral arterial puncture for coronary arterial angiography and PCI were treated with UGTI. One coronary arterial angiography and 14 PCI were performed in 5 males and 10 females. Nine simple PA with one lobe and 6 complex PA with two or three lobes. PA form,size,neck length and width,thrombin dose, therapic effect and complications were analysed. Duplex sonographic follow-up examination was performed at 24 hours and 5-7 days. Results Mean volume of PA was (13?5)cm 3. Twenty-one thrombin injections were performed. Mean thrombin dose was (250?120) IU in simple and (650?150) IU in complex PA. Primary success rate was 100% for 9 simple and 66.7 % for 4 of 6 complex PA. Recurrence occurred in one complex PA after 24 hours of UGTI and another complex PA reappear was detected after 15 days of UGTI, PA were completely obliterated after 3 times thrombin injections. Secondary success rate was 100%. No thromboembolic,infectious,allergic complications occurred. Conclusions UGTI is safe and effective as first-line therapy of iatrogenic pseudoaneurysms following femoral arterial puncture for PCI. Geometry form of PA is determinant factor of primary success rate.

Objective:To study the characters of perioral muscle p re ssure of the adult patients with skeletal crossbite. Methods:20 patients were diagnosed to be with skeletal crossbite. The perioral muscle press ures on upper and lower central incisors, first molars and cuspids were measured buccally and lingually at rest position. 10 health adults were served as the co ntrols. Results:The perioral force in skeletal crossbite group was larger than that in the control group on the correspondence area(P0.05). In the skeletal cross bite group, the perioral force on the mandibular area was larger than that on th e correspondence maxillary area(P0.05). The muscle pressure on the labial side was larger than that on t he lingual correspondence side in both groups(P

This study was performed to assess the accuracy of pulsed Doppler echocardiographic(PDE) diagnosis of ventricular septal defect(VSD). 119 children with congenital heart disease, aged 9 months to 17 years, admitted to our hospital for cardiac surgery, were studied. These children had VSD(56), ASD(18), PDA(43), PS(1) and Cortriatrium(1). A specific PDE diagnosis of VSD can be made by following the turbulent VSD jet(TVJ) through the septum, and a PDE diagnosis of VSD was made in 53 out of 56 patients(95%) proven by catheterization and operation. There were two false positive PDE diagnosis of VSD by PDE, were proven to be perimembranous VSD at operation, and 3 children who were classified into subarterial infundibular VSD by PDE, were proven to be perimembranous VSD at operation. PDE seems to be a very useful tool in preoperative evaluation of VSD.
Catheterization ; Catheters ; Child ; Diagnosis* ; Echocardiography, Doppler, Pulsed* ; Heart Defects, Congenital ; Heart Septal Defects, Ventricular* ; Humans ; Thoracic Surgery

Objective To discuss the safety and efficacy of laparoscopic modified ventrifixation for women with uterine prolapse.Methods Thirty-seven women with Ⅱ-Ⅳ degree uterine prolapse were undergent laparoscopic modified ventrifixation in the the First Hospital of Qinhuangdao and Anzhen Hospital of Beijing from January 2008 to June 2012.The midpart of a noabsorble PROLENE soft mesh was sutured to the anterior cervical fascia.Two back ends of the mesh were passed through extraperitoneal channels through ligamentum latum uteri and sutured to the abdorminal wall to fix uterus.The effect and complications were observed.Results The objective and subjective success rate were 100％ and 91.9％ respcetively at 6 months after operation.All patients were followed up for 6 to 48 months.Prolapse recurrence rate was 10.8％ (4/37).Conclusion Laparoscopic modified ventrifixation is effective,safe and mini-invasive in the treatment of uterine prolapse.The surgery may be a satisfactory procedure for women with uterine prolapse hoping for uterine preservation.

Objective To summarize and analyze the emergency management for acute renal failure of solitary kidney induced by upper urinary calculi. Methods A total of 78 cases of acute renal failure of solitary kidney induced by upper urinary calculi were included.The mean course was 2.8 days (range,1 to 8 days).The time from onset to emergency management was within 72 hours in 68 out of the 78 cases.10 cases were treated with percutaneous nephrostomy drainage (PCN) under the guidance of B-ultrasound;25 cases with emergent open surgery;12 cases with indwelling ureteral stent retrogradely;and 31 cases were treated with transurethral ureteroscopy ballast lithotrity. Results One died of bleeding and infection after operation.In 65 cases treated with in 72 hours after obstruction, the serum Cr level decreased from 202～885 ?mol/L to 58～130 ?mol/L;the serum BUN level decreased from 9.8～42.4 mmol/L to 2.8～7.0 mmol/L after urgent treatment during a period of 3 to 12 days,and the renal functions recovered. Conclusions The principles of management for this acute condition should be diagnosing early and promptly,releasing obstruction as soon as possible,preventing complications effectively and reserving renal functions.KUB and B-ultrasonography are the first choice of diagnostic measures.Transurethral ureteroscopy ballast lithotrity can be recommended as the first choice of emergent treatment.

Administration, Inhalation ; Endothelium-Dependent Relaxing Factors ; pharmacology ; Humans ; Hypertension, Pulmonary ; drug therapy ; Nitric Oxide Donors ; administration & dosage ; therapeutic use ; Nitroglycerin ; administration & dosage ; therapeutic use ; Nitroprusside ; administration & dosage ; therapeutic use ; Phosphodiesterase Inhibitors ; administration & dosage ; therapeutic use

Objective It was demonstrated that pulsatile perfusion (PP) is more physiologic and better than non-pulsatile perfusion (NPP) in maintaining blood circulation in vital organs. The purpose of this study was to determine the effect of PP on cerebral cortical blood flow(CCBF) after deep hypothermic circulatory arrest(DHCA) and the duration of safe DHCA. Methods Thirty-nine adult healthy mongrel dogs of either sex, weighing 10-15 kg were divided into two groups: PP group (n = 18) and NPP group (n = 21) . The animals were anesthetized with intravenous pentobarbital 25mg?kg-1 . After tracheal intubation the animals were mechanically ventilated. Right femoral artery and vein were cannulated for intra-arterial pressure monitoring and infusion. The chest was opened and heart exposed. CPB was started after insertion of venous drainage catheter into right atrium and arterial cannula into ascending aorta. The bypass pump was shut off when the brain was cooled to 20℃. Circulatory arrest was maintained for 40 min, 60min or 80 min respectively. CCBF was measured during cooling and rewarming at 35℃, 30℃, 25℃and 20℃ by hydrogen clearance technique. Results During cooling CCBF gradually decreased and there was no difference in CCBF between the groups. After 40 min DHCA during rewarming CCBF increased significantly faster in PP group than in NPP group during the early period but CCBF returned to pre-cooling baseline level at the end of rewarming in both groups. After 60 min DHCA during rewarming when brain temperature returned to 30℃ and above , CCBF increased significantly faster in PP group than that in NPP group and returned to pre-cooling baseline level at the end of rewarming in both groups. Electron microscopic examination revealed that ultrastructure of neurons was normal after 40 min DHCA in both groups. After 60 min DHCA the ultrastructure was normal in PP group but swelling of neurons andedema of mitochondria could be seen in NPP group. After 80 min DHCA marked neuronal damages could be seen in both groups.Conclusions The results of our study suggest that PP improves CBF after DHCA and can protect brain from ischemic and hypoxic damages induced by DHCA and the duration of safe DHCA at 20℃ should be less than 60 min.