STUDY DESIGN: Changes of morphology and phenotype of cultured cells in media added lactate were observed. OBJECTIVES: To evaluate the effect of lactate on morphology and phenotype of cultured intervertebral disc cell. SUMMARY OF LITERATURE REVIEW: It was reported that lactate and pH were important factor in the degeneration of intervertebral disc. However the effect of lactate on morphology and phenotype of cultured intervertebral disc cell have not been studied. MATERIALS AND METHODS: Cells were dissociated enzymatically from rabbit nucleus pulposus. After attaining monolayer growth, the cells were incubated in media added 2mM or 5mM lactate. Total cell counts and morphological changes of the cells were periodically observed. Changes in cell phenotype were investigated by use of anti-collagen antibody stain. RESULTS: The cell groups added no lactate and 2mM lactate showed no difference in cell counts, morphology and phenotype. The cell group added 5mM lactate showed a reduction in final cell Counts and highel'ratio of fibroblastic cell in total population. Anti-collagen I Ab stained the Intra-and extra-cellular area of fibroblastic cells and intracellular area of chondrocytic cells. CONCLUSIONS: The current study suggests that high concentration of lactate inhibit intervertebral disc cell proliferation and accelerate morphological and phenotypical change to fibroblastic cell.
Cell Count ; Cell Proliferation ; Cells, Cultured ; Fibroblasts ; Hydrogen-Ion Concentration ; Intervertebral Disc* ; Lactic Acid* ; Phenotype*

No abstract available.
Humans

We experienced a case of adenocarcinoma arising in sacrococcygeal teratoma. The patient was a 52-year-old woman. She was admitted due to one month of sacral pain. She had a sacral mass since birth. On physical examination, anal fistula was present at the perianal area and pus drainage was noted. MR image showed multiple variable-sized cysts with inhomogeneous density. Resected specimen, mesuring 12.5 7.0 cm in diameter, showed multiple variable-sized cystic lesions admixed with grayish solid portion. The cysts contained mucoid material. The microscopic examination showed mature teratoma composed of cysts lined by pseudostratified ciliated columnar epithelium, intestinal mucosa, mature cartilage, bone, and fat tissue. A moderately differentiated adenocarcinoma developed from the cystic area in the mass.
Adenocarcinoma* ; Adult ; Cartilage ; Drainage ; Female ; Humans ; Intestinal Mucosa ; Middle Aged ; Mucous Membrane ; Parturition ; Physical Examination ; Rectal Fistula ; Suppuration ; Teratoma*

No abstract available.
Depression, Postpartum* ; Female ; Postpartum Period*

The insertion of nasogastric tubes in comatose, obtunded or anesthetized patients is often difficult, frustrating and time-consuming. A large variety of methods inserting nasogastric tubes in those uncooperative patients have been reported. As a new effective method, we used Savary-Gilliard Wire Guide(R), which is designed for introducing Savary-Gilliard Dilator(R) into a strictured esophagus, for inserting a nasogastric tube in a comatose patient who was intubated with a ballooned tracheostomy tube. The insertion was successful in the first attempt and no complication occurred.
Coma* ; Esophagus ; Humans ; Tracheostomy

PURPOSE: We investigated the epidemiological and clinical characteristics of leisure activity- and sports-related ocular trauma for application to its treatment and prevention. METHODS: A retrospective chart review was conducted on 121 patients who visited Gachon University Gil Medical Center from January 2006 to December 2007 because of leasure activity- and sports-related ocular trauma. The incidence of ocular injuries, sex and age, monthly and seasonal distribution, causes, diagnosis, and visual acuity were reviewed. RESULTS: Among the 1592 patients of ocular trauma, 121 patients (7.6%) were related to leasure activity- and sports. The incidence was higher in males (81.8%; 4.5-fold) than in females. The mean age of patients was 23.7+/-7.1 years. Ocular injuries occurred least in winter (19.8%) and occurred more during the week than on weekends (1.6-fold). The most common cause was soccer (39.7%), more specifically being hit with the soccer ball (47.1%). The initial LogMAR visual acuity was worse in the females and in the patients older than 30 years. Traumatic hyphema (19.7%) was the most common cause of visual disturbance, and corneal erosion (11.3%) occurred frequently. CONCLUSIONS: Leasure activity- and sports-related ocular trauma is becoming an increasingly significant cause of ocular morbidity, and trends relating to characteristics such as sex and age were found. These outcomes can be put to practical use in the prevention and treatment of sports-relatedocular injuries.
Female ; Humans ; Hyphema ; Incidence ; Leisure Activities ; Male ; Retrospective Studies ; Seasons ; Soccer ; Sports ; Visual Acuity

The purpose of this study was to evaluate the postoperative outcomes between the glaucoma triple procedure and the small incision cataract surgery using foldable silicone intraocular lens(IOL). This prospective randomized study comprised of 42 eyes which had the glaucoma triple procedure and 41 eyes which underwent small incision cataract surgery at chonnam University Hospital from May, 1994 to December, 1996. At least 3 months follow-up period was needed. Corrected visual acuity of 0.5 of better was obtained in 50% of triple procedure group and 88% of small incision cataract surgery group with the average lines of improved visual acuity of 5.6 and 7.2 respectively. There was no significant change in the refractive cylinder during the early postoperative period in both group. In the glaucoma triple procedure group, posterior capsular opacification(PCO) developed in significantly low incidence in the Mitomycin C using group and the incidence of PCO in eyes with IOL decentration was greater than in those without IOL decentration. In conclusion, patients who underwent glaucoma triple procedure using foldable silicone IOL showed excellent postoperative IOP control with success rate of 81%, rapid visual rehabilitation and refractive stability. We also found that glaucoma triple procedure with Mitomycin C was associated with lower incidence of PCO.
Cataract* ; Follow-Up Studies ; Glaucoma* ; Humans ; Incidence ; Jeollanam-do ; Mitomycin ; Postoperative Period ; Prospective Studies ; Rehabilitation ; Silicones* ; Visual Acuity

AIDS, which is being spreaded world-wide, would have the number of the patients and the infected persons to several million persons since the first patient had been reported among the homosexualists in 1981, and in our country patients of 323 persons had been found till 1993, therfore we could have significant concern and warning. Accordingly, this writer intended to present basic data to the blood Project and AIDS preventive movement by investigating knowledge, attitude and concern regarding AIDS including blood donors, and analyzed data of 1,920 pages by questionaire method from March to August 1994. According to the results, it is rather well-known as far as general knowledge, attitude and concern regarding AIDS are concerned, however, items which are 20-40% wrongly known are as followings: AIDS is infected by virus. It is infected by insects such as mosquito. It is infected with tight kiss with the person infected with AIDS. Risk of being infected with AIDS will be reduced if using condom when sexual intercourse. It may be infected through the foods. It may be infected by the same bacteria attacking venereal disease. And WHO(world Health Organization) designated Dec. 1st as AIDS'Day. Items which are 40-60 wrongly known are as followings: AIDS can be infected by blood donation, Now vaccine preventing AIDS has been developed. In our country, Preventive law of AIDS is established. And in the field of the attitude and concern, it was surveyed as important as being hahdied by the broadcasting and newspaper, and 60470% of the persons answered that they would avoid AIDS infected person, and 76% of male and 87.1% of female gave the reply thet AIDS infected persons should be administered extraordinarily by isolated accommodation by the nation.
Bacteria ; Blood Donors* ; Coitus ; Condoms ; Culicidae ; Female ; Humans ; Insects ; Jurisprudence ; Male ; Periodicals ; Sexually Transmitted Diseases ; World Health Organization

OBJECTIVE: We previously described that Diva is highly expressed in matured metaphase II (MII) oocytes compared to immature germinal vesicle (GV) oocytes in mouse.1 We report here that the expression of Diva transcript as well as protein is oocyte-specific. To elucidate its physiological role in oocyte, the binding partner(s) of Diva has been identified by using immunoprecipitation (IP) followed by Mass Spectrometry. METHODS: NIH/3T3 cells were transiently transfected for 24 h with either empty vector for control or FLAG-tagged mouse Diva construct, and IP was performed with anti-FLAG antibody. The immuno-isolated complexes were resolved by SDS-PAGE on a 12% gel followed by Coomassie Blue staining. For in-gel digestion, 15 bands of interest were excised manually and digested with trypsin. All mass spectra were acquired at a positive reflector mode by a 4700 Proteomics Analyzer (Applied Biosystems, Framingham, MA). Proteins were identified by searching the NCBI nonredundant database using MASCOT Peptide Mass Fingerprint software (Matrixscience, London). RESULTS: Diva-associated complexes were formed in FLAG-tagged mouse Diva-overexpressed NIH/3T3 cells via IP using anti-FLAG-conjugated beads. Among the excised 15 bands, actin and actin-binding proteins such as tropomyosin, tropomodulin 3, and alpha-actinin were identified. Binding between Diva and actin or tropomyosin was confirmed by IP followed by Western blot analysis. Both bindings were also detected endogenously in mouse ovaries, indicating that Diva works with actin and tropomyosin. CONCLUSIONS: This is the first report that immuno-isolated Diva-associated complexes are related to actin filament of the cytoskeletal system. When we consider the association of Diva with actin and tropomyosin, oocyte-specific Diva may play a role in modulating the cytoskeletal system during oocyte maturation.
Actin Cytoskeleton ; Actinin ; Actins ; Animals ; Blotting, Western ; Dermatoglyphics ; Digestion ; Electrophoresis, Polyacrylamide Gel ; Female ; Immunoprecipitation ; Mass Spectrometry* ; Metaphase ; Mice ; Microfilament Proteins ; Oocytes ; Ovary ; Proteomics ; Tropomodulin ; Tropomyosin ; Trypsin

A 38-year-old female patient presented dystrophic nail changes on her all finger and toe nails for 10 years. On examination, nails were uniformly affected with longitudinal ridging, loss of normal luster and brownish discoloration. This patient has not had any inflammatory skin diseases or systernic illness for the past 10 years. Biopsy examination from the dystrophic nail showed non-specific eczematous changes. The clinical and histologic features of this yatient was considered as the consistent findings with the ty enty-nail dystrophy, occured on the idiopathic base, coined by previous authors.
Adult ; Biopsy ; Female ; Fingers ; Humans ; Numismatics ; Skin Diseases ; Toes