The antigenic domain of the major surface protein (Nc-p43) of Neospora caninum was examined by polymerase chain reaction of its gene fragments and recombinant expression as GST fusion proteins. The fragments of Nc-p43 were as follow: a total open reading frame (OFR), T; OFR without signal sequence and C-terminal hydrophobic sequence, S; N-terminal 2/3 parts of S, A; C-terminal 2/3 parts, P; N-terminal 1/3 part, X; middle 1/3 part, Y; and C-terminal 1/3 part, Z, respectively. The DNA fragments were cloned into pGEX-4T vector. Recombinant plasmids transformed into Escherichia coli of BL21 pLysS (DE3) strain were induced to express GST or GST fused fragments of Nc-p43 such as 69 kDa protein for T, 66 kDa for S, 52 kDa for A, 53 kDa for P, and 40 kDa proteins for X, Y, and Z, respectively in SDS-PAGE. The Nc-p43 fragments of T, S, and P reacted with a bovine serum of neosporosis while those of A, X, Y, and Z together with GST did not in the western blot. These findings suggest that the antigenic domain of Nc-p43 of N. caninum may be localized in the C-terminal 2/3 parts. Together with A19 clone in SAG1 of Toxoplasma gondii (Nam et al., 1996), the P fragment of Nc-p43 could be used as efficient antigens to diagnose and differentiate those infections with both species.
Amino Acid Sequence ; Animals ; Base Sequence ; Biological Markers/analysis ; Blotting, Western ; Cells, Cultured ; Cercopithecus aethiops ; Coccidiosis/diagnosis ; Neospora/*immunology ; Protozoan Proteins/*analysis/genetics/immunology ; Reverse Transcriptase Polymerase Chain Reaction ; Support, Non-U.S. Gov't ; Vero Cells/parasitology

No abstract available.
Snake Bites* ; Snakes*

Voice changes developing after endotracheal intubation during right hemicolectomy with endotracheal intubation have been found to be due to a right recurrent laryngeal nerve palsy in 43-years-old male patient. It was likely that the inflated cuffed tube rode up to the level of the cricoid cartilage during the course of surgery as traction was placed on the endotracheal tube because the condenser humidifier and breathing circuit weighed heavy. Cuff overexpansion, in addition to muscle relaxation and decreased tracheal elasticity were considered as contributing factors of vocal cord palsy. We believe that tube traction and cuff overexpansion were the mechanism of vocal cord palsy in our patient. So we recommend the routine use of tube stand so that weigh of the breathing circuit does not transmit traction to the endotracheal tube. Concurrently, filling the cuff with a sample of the inspired mixture of gases, saline and 4% lidocaine in special cases or regular deflation of the cuff must be considered.
Cricoid Cartilage ; Elasticity ; Gases ; Humans ; Intubation, Intratracheal* ; Lidocaine ; Male ; Muscle Relaxation ; Respiration ; Traction ; Vocal Cord Paralysis* ; Vocal Cords* ; Voice

No abstract available.
Abdomen, Acute* ; Aged* ; Geriatrics* ; Humans

Large-cell acanthoma is a generally hyperkeratotic, sharply demarcated patch on sun-exposed skin with the outstanding pathologic feature being composed of large, relatively uniform keratinocytes. We describe a case of large-cell acanthoma that involved the skin of the nasal bridge. Patient was a 56-year-old women with a tannish brown patch, 2 cm in size and of 5 years' duration. Controversial issues about nosologic entity of large cell acanthoma are discussed.
Female ; Humans

To know wheter the injury of the ligament may induce the meniscal tear, we statistically analyzed the incidence of meniscal tear with χ2 in cases of fresh and old(more than 3 months since injury) ligament injury in 419 joints of 407 adults who had been treated during the time between early 1984 and the end of 1992 in St. Mary's Hospital. The results were as follows: 1. The meniscal tear occurred more frequently in old ligament tear(44/70, 62.9%) than in fresh ligament tear(7/26, 26.9%) in cases of isolated ACL tear(P=0.062). 2. There was no significant difference between the old(10/34, 29.4%) and the fresh tear(24/96, 25.0%) in the combined ACL tear(P=0.615). 3. Overall ACL tear patients showed higher incidence of meniscal tear in the old ligament tear(54/1-4, 51.9%) than in the fresh ligament tear(31/122, 25.4%)(P=0.000). 4. The frequency of meniscal tear was very low in the isolated PCL tear(fresh tear: 0/14, old tear:1/17, 5.9%). In cases of combined PCL tear the incidence was rather higher in the fresh tear(11/33, 33.3%) than in the old tear(2/30, 6.7%)(P=0.015). 5. Overall PCL tear patients showed higher incidence of meniscal tear in the fresh ligament tear(11/47, 23.4%) than in the old ligament tear(3/47, 6.4%)(P=0.020). 6. The meniscal tear occurred more frequently in the ACL tear than in the PCL tear in both isolated(ACL : 85/96, 53.1%, PCL : 1/31, 3.2%) and overall ACL or PLC tear group(ACL : 85/226, 37.6%, PCL : 14/16.7%)(P=0.000). 7. The meniscal tear occurred less frequently in the isolated clootgeral ligament tear(9/94, 9.6%) than in the isolated cruciate ligament tear(52/127, 40.9%)(P=0.000). 8. The incidence of tear between the medial and the lateral meniscus showed no statistically significantly difference(P=0.215). From these results it would be suggested that the ACL tear, especially isolated tear may induce meniscal tear. On the other hand there was no statistically significant relationship between the incidence of meniscal tear and those of either PCL tear or collateral ligament tear.
Adult ; Collateral Ligaments ; Hand ; Humans ; Incidence ; Joints ; Ligaments ; Menisci, Tibial ; Tears

Although cystic neoplasms of the pancreas are an uncommon disease, it occures often enough that proper sugicalmanagement depends on accurate preoperative diagnosis. It is very difficult to diagnose accurately by conventionalradiological methods alone. Angiographic findings are fairly characteristic for these tumors, but they are in noway specific. The imaging characteristics of ultrasonography and CT are particularly useful in the diagnosis ofthis particular tumors. This report presents an analysis of 2 cases of cystadenoma and 3 cases cystadenocarcinomaexamined by ultrasonography and CT. Ultrasonography findings are complelx multicytic mass in the pancreas withthick irregular wall, daughter cyst, septa and solid component. These septa and solid component are highlyechogenic if the tumor is mucin producing. Computed tomographic findings are large cyst possessing irregular thickwall, solid component and septa which are enhanced by contrast infusion. Punctate or curvilinear calcification isseen occasionally. Tortuous or beaded vascular collaterals are seen around the cyst. On the other hand, pseudocystis usually a single cyst possessing thin smooth wall, and solid component or septa are unusual in pseudocyst.
Cystadenocarcinoma ; Cystadenoma ; Diagnosis ; Hand ; Mucins ; Nuclear Family ; Pancreas ; Ultrasonography

Discoid medial meniscus was first reported in l930 by Watson-Jones. Discoid medial menisci are very rarely reported although the presence of discoid lateral menisci are not uncommon. From Jan. 1989 to Dec. 1994, we have experienced five cases who had discoid medial menisci with pain due to substance tear following knee injury in arthroscopy. There were three complete type anct two incomplete type. Out of flve cases, longitudinal tea1 and bucket-handle type tear were two, respectively. The remained one was a compound tear.
Arthroscopy ; Knee Injuries ; Menisci, Tibial*

No abstract available in English.
Adult ; Clinical Study ; Fractures, Comminuted ; Humans

Prostatic adenocarcinoma is a common genitourinary malignancy which frequently is assiciated with bony metastasis at initial diagnosis. The detection of bony metastasis is important because it often alters the therapeutic strategy. The radionuclide one scintigraphy is known to be the most sensitive diagnostic method in detecting bony metastasis from prostatic adenocarcinoma. In the histologic grading of prostatic adenocarcinoma, the Gleason's grading system is the most widely employed one which is based on the degree of glandular differentiation and the growth patterns of tumor in relation to the prostatic stroma. The purpose of this study is to know the correlation of Gleason's grade and bone scintigraphic findings in prostatic carcinoma. We analyzed 99mTc-diphosphonate bone scintigraphies of 19 cases with prostatic adenocarcinoma confirmed by transurethral prostatic biopsy. Ten of the 19 cases showed asymmetrical multiple radioisotope uptake suggesting bony metastases and their Gleason's grade were 4 or 5 in seven cases. The remaining three cases were Gleason's grade 2 or 3. Another nine cases showed symmetrical radioisotope uptake with normal renal imaging suggesting no bony metastases and their Gleason's grade were 1,2 or 3 in seven cases. The remaining two cases of these nine cases were Gleason's grade 4 and 5. As the results, there was a correlation between the Gleason's grade and bone scintigraphic findings. The asymmetrical multiple skeletal uptake of radioisotope may suggest that the Gleason's grade is likely to be higher.
Adenocarcinoma ; Biopsy ; Diagnosis ; Methods ; Neoplasm Metastasis ; Radionuclide Imaging