The aim of the current study was to demonstrate the potential therapeutic efficacy of resveratrol in oral cancer patients. Lysophosphatidic acid (LPA) intensifies cancer cell invasion and metastasis, whereas resveratrol, a natural polyphenolic compound, possesses antitumor activity, suppressing cell proliferation and progression in various cancer cell lines (ovarian, gastric, oral, pancreatic, colon, and prostate cancer cells). In addition, resveratrol has been identified as an inhibitor of LPA-induced proteolytic enzyme expression and ovarian cancer invasion. Furthermore, resveratrol was shown to inhibit oral cancer cell invasion by downregulating hypoxia-inducible factor 1α and vascular endothelial growth factor expression. Recently, we demonstrated that LPA is important for the expression of transcription factors TWIST and SLUG during epithelial-mesenchymal transition (EMT) in oral squamous carcinoma cells. In this study, we treated serum-starved cultures of oral squamous carcinoma cell line YD-10B with resveratrol for 24 hours prior to stimulation with LPA. To identify an optimal resveratrol concentration that does not induce apoptosis in oral squamous carcinoma cells, we determined the toxicity of resveratrol in YD-10B cells by assessing their viability using the MTT assay. Another assay was performed using Matrigel-coated cell culture inserts to detect oral cancer cell invasion activity. Immunoblotting was applied for analyzing protein expression of SLUG, TWIST1, E-cadherin, and GAPDH. We demonstrated that resveratrol efficiently inhibited LPA-induced oral cancer cell EMT and invasion by downregulating SLUG and TWIST1 expression. Therefore, resveratrol may potentially reduce oral squamous carcinoma cell invasion and metastasis in oral cancer patients, improving their survival outcomes. In summary, we identified new targets for the development of therapies against oral cancer progression and characterized the therapeutic potential of resveratrol for the treatment of oral cancer patients.
Apoptosis ; Cadherins ; Carcinoma, Squamous Cell ; Cell Culture Techniques ; Cell Line ; Cell Proliferation ; Colon ; Epithelial-Mesenchymal Transition ; Gastropoda ; Humans ; Immunoblotting ; Lysophospholipids ; Mouth Neoplasms* ; Neoplasm Metastasis ; Ovarian Neoplasms ; Prostatic Neoplasms ; Stilbenes ; Transcription Factors ; Vascular Endothelial Growth Factor A

BACKGROUND: High-frequency jet ventilaion is considered a reliable technique for anesthesia and critical care including respiratory failure but there are adverse reactions such as carbon dioxide retension and dry of respiratory mucosa. The purpose of this study was to confirm the effects of combined high- frequency jet ventilation (HFJV) and converntional mechanical ventilation (CMV) on the cardiovascular system, arterial blood gases tension and mean airway pressure in 9 Korea mongrel dogs with pulmonary edema induced by oleic acid. METHODS: During CMV with 20 breaths/minute, 10 ml/kg of tidal volume and F1O2 1.0, parameers were evaluated (base line value). When pulmonary edema was developed, HFJV was applied initially with 120 breaths/minute, inspiratory time 30% and driving pressure 40 psi F1O2 1.0 for 60 minutes (control value) and thereafter simultaneous use of CMV was applied with the tidal volume of 10 ml/kg and each respiratory rate 8, 4, 2, 1, 0.5 per minute for 30 minutes. RESULTS: Combined application of HFJV and CMV (above repiratory rate 1 per minute) achieved the improvement of oxygenation and carbon dioxide elimination, and Paw was decreased without undesirable effects on cardiovascular system in case of the induced pulmonary edema. CONCUSIONS: From above results we recommanded that HFJV combined with CMV may be a useful method of treatment for respiratory failure.
Anesthesia ; Animals ; Carbon Dioxide ; Cardiovascular System ; Critical Care ; Dogs* ; Gases ; High-Frequency Jet Ventilation* ; Korea ; Oleic Acid* ; Oxygen ; Pulmonary Edema* ; Respiration, Artificial* ; Respiratory Insufficiency ; Respiratory Mucosa ; Respiratory Rate ; Tidal Volume ; Ventilation

Malignant fibrous histiocytoma of the bone is relatively rare than that affecting the soft tissue. The authors have first experienced a case of malignant fibrous histiocytoma affecting the distal tibia of thirty-two years old woman in Korea. We obtained good results from free vascularized fibular graft after radical resection in stead of amputation. A case of malignant fibrous histiocytoma of the bone is reported with brief review of literature.
Amputation ; Female ; Histiocytoma, Malignant Fibrous ; Humans ; Korea ; Tibia ; Transplants

Arthroscopy of the knee joint is an essential diagnostic procedure of the knee joint lesions because of its high diagnostic accuracy and simple procedure and high safety. We analysed in 300 cases of knee joints arthroscopy which performed in Kyung Hee University Hospital from July 1978 to June 1982. The results were as follows: 1. The diagnostic accuracy of clinical diagnosis of knee joint lesions based on arthroscopic findings was 51.0%. 2. The diagnostic accuracy of clinical diagnosis in surgically confirmed cases of knee joint lesions was 56.1% and diagnostic accuracy of arthroscopy was 90.9%. 3. In meniscal lesions, the diagnostic accuracy of arthroscopic diagnosis was 94.5%, arthrographic diagnostic accuracy was 84.9%, clinical diagnostic accuracy was 48.9% and combined accuracy of three diagnostic methods was 97.3%. 4. In cases which diagnosed as I.D.K. (Internal Derangement of Knee) in initial stage of knee joint lesions, meniscal lesions (32.8%) and ligament injuries (22.4%) were predominant lessions based on arthroscopic findings. 5. The frequency of meniscal tear were no significant difference in lateral (33.3%) or medial (36.0%) meniscus except discoid meniscus rupture. 6. 68 Cases of 300 arthroscopies (22.7%) were avoided unncessary arthrotomy through preoperative arthroscopic examination of knee joint. 7. Arthroscopy is very useful and essential not only to confirm the diagnosis but also to treatment of knee joint lesions.
Arthroscopy ; Diagnosis ; Knee Joint ; Knee ; Ligaments ; Rupture ; Tears

A double-blind trial of the efficacy of propranolol hydrochloride a new beta adrenergic blocking agent, in the prophylactic treatment of angina pectoris was conducted in 9 patients. Through the trial period of 8 weeks to more than 26 weeks, the patients received propranolol and placebo for equal period. Following are the results. 1. The effective dose of propranolol hydrochloride in the prophylactic treatment of angina pectoris was 80 mg to 120 mg per day. 2. The effectiveness of propranolol hydrochloride was manifested by a reduction in frequency of pain to 40.7%, in nitroglycerin comsumption to 38.8% and by an increase in exercise tolerance. 3. Obseved side effects were transient nausea, vomiting and bradycardia, which were improved with reduction in drug dosage. 4. Further clinical and experimental information may be necessary in order to assess the long-term effects of propranolol hydrochloride.
Angina Pectoris* ; Bradycardia ; Exercise Tolerance ; Humans ; Nausea ; Nitroglycerin ; Propranolol* ; Vomiting

Administration of succinylcholine for intratracheal intubation may cause untoward reactions such as elevation of intraocular and intrabdominal pressure, increased plasma potassium level, and development of postoperative muscle pain, ets. The fasciculation of muscle fibers caused by nondepolarizing activity of succinylcholine may be responsible for those reactions, although this is not clearly defined. Several attempts ehterfore, have been made to minimize the development of muscle fasciculation following administration of succinylcholine. Of thest, self-taming of succinylcholine, pretreatment wit small doses of non-depolarizing neuromuscular blockers, or the use of hexafluorenium are examples. The authors observed the effects of succinylcholine on intraocular pressure and the degree of muscle fasciculation after self taming of succinylcholine. pretreating the patients with d-tubocurarine and pancuronlum before administration of succinylcholine was also studied. All three techniques showed significant decreases in the degree of muscle fasciculation. And small doses of d-tubocurarine and pancuronium did not seem to affect intraocular pressure, although a taming dose of succinylcholine itself caused significant increases in intraocular pressures.
Fasciculation ; Humans ; Intraocular Pressure ; Intubation, Intratracheal ; Myalgia ; Neuromuscular Blockade ; Neuromuscular Blocking Agents ; Pancuronium ; Plasma ; Potassium ; Succinylcholine* ; Tubocurarine

Thirty consecutive patient seen in a pediatric out patient and emergency department, in whom the diagnosis of intussusception was considered, had an ultrasound examination of the abdomen before the barium enema. The peak age was 5~12 month (range 4~34 month). Intussusception was detected by ultrasonography in all 25 cases proved by barium enema (sensitivity 100%). Normal findings on ultrasonography correlated with a negative barium enema results in 5 of 5 cases (negative predictive value=100%). No intussusception was missed by ultrasonography. To determine which patient would most benefit from ultrasonography, we divided patients into either a high risk group (100% intussusception) or a low risk group (37.5% intussusception) on the basis of clinical symptoms. We conclude that ultrasonography can be used as a rapid sensitive screening procedure in the diagnosis or exclusion of childhood intussusception. Probability of surgical reduction was associated with size of of total thickness and external rim thickness.
Abdomen ; Barium ; Child* ; Diagnosis* ; Emergency Service, Hospital ; Enema ; Humans ; Infant* ; Intussusception* ; Mass Screening ; Ultrasonography*

Arthroscopy assisted anterior cruciate ligament(ACL) reconstruction with the autogenous bone-patellar tendon-bone(BPB) graft showed more favorable results in the hand of experienced surgeon, but it requires long leaming curve for skillful techniques. On the contrary, open method is very easy to learn, but it needs more soft tissue dissection affecting rehabilitation and morbidity. Arthrotomy through the patellar tendon harvest site provide good surgical approach without supplementary incision, and hence short operation time and good clinical result can be expected. The purpose of this study is to introduce this technique and compares the results with arthroscopic one. We performed 67 cases of ACL reconstruction with autogenous BPB graft through the patellar tendon harvest site. We recorded and analysed the physical findings, Lysholm score and Cybex test at preoperative and postoperative 3, 6, 12 months. Average Lysholm score was 58.0 preoperatively and 90.5 at postoperative one year. Cybex test at one year follow-up showed decreased muscle strength of quadriceps, which was nearly the same result with arthroscopic technique. In conclusion, this technique provided similar excellent clinical results comparing with the arthroscopy assisted ones. Furthermore, this technique had merits of short learning curve and short operation time.
Anterior Cruciate Ligament Reconstruction* ; Anterior Cruciate Ligament* ; Arthroscopy ; Follow-Up Studies ; Hand ; Learning Curve ; Muscle Strength ; Patellar Ligament* ; Rehabilitation ; Transplants

Spontaneous intra-abdominal hemorrhages are uncommon in hemophilic patients. They can cause complications in patients with severe hemophilia, and are associated with a high mortality rate. To date, there is no guideline for the management of intra-abdominal hemorrhage in patients with hemophilia. Management of intra-abdominal hemorrhage ranges from conservative treatment to emergent embolization or surgery. We describe two children with hemophilia A, who were successfully treated non-operatively by administering coagulation factor concentrates and embolization, and were later discharged from the hospital. We emphasize the role of an active approach in the evaluation and management of intra-abdominal hemorrhage without any surgical intervention.
Blood Coagulation Factors ; Child ; Hemophilia A ; Hemorrhage ; Humans ; Mortality

No abstract available.