1.Polysaccharides isolated from Phellinus gilvus enhances dermal wound healing in streptozotocin-induced diabetic rats.
Jae Sung BAE ; Kwang Ho JANG ; Hee Kyung JIN
Journal of Veterinary Science 2005;6(2):161-164
Dermal wound healing is a complex process that involved inflammation leading to re-epithelialization, granulation tissue, and tissue remodeling. Previous studies from our laboratory have shown that polysaccharides isolated from fungus, Phellinus gilvus (PG) have various anti-inflammatory activities. In present study, we have assessed the effect of polysaccharides from PG on the dermal wound healing of polysaccharides from PG in streptozotocin-induced diabetic rat model. Six of 6-mm circular wounds were created with biopsy punch on the 4th day after induction of diabetes. After 24 hours, each test substance was applied to the wound twice a day for next 5 days. Circular wounds treated with PG showed significantly reduced wound contraction and complete reepithelialization, as compared to wounds of non-treated (p < 0.05). These results show that polysaccharides isolated from PG enhanced wound repair in diabetic impaired healing, and could be developed as a wound healing agent in such clinical settings.
Administration, Cutaneous
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Animals
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Anti-Inflammatory Agents/pharmacology
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Basidiomycota/*metabolism
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Diabetes Mellitus, Experimental/*pathology
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Histocytochemistry
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Male
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Polysaccharides/isolation&purification/*pharmacology
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Rats
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Rats, Sprague-Dawley
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Skin/*injuries
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Streptozocin
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Wound Healing/*drug effects
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Wounds, Penetrating/*drug therapy
2.Evaluation of the anaerobic bacteria from the clinical specimens.
Kyungwon LEE ; In Ho JANG ; Wonkeun SONG ; Young Jin KIM
Korean Journal of Clinical Pathology 1991;11(3):615-625
No abstract available.
Bacteria, Anaerobic*
3.Transcutaneous Dlectrical Nerve Stimulation (TNS or TENS) for Relief of Pain in Herpes Zoster.
Jang Ho BYEN ; Sae Jin YOON ; Joong Hwan KIM
Korean Journal of Dermatology 1981;19(1):65-68
The gate control theory was proposed by Melzack & Wall in 1965. This theory implies that selective stimulation of cutaneous afferent fibers of large diameter inhibits pain. Transcutaneous electrical stimulation(TNS) is a practical employment of this concept. TNS was used on 14 herpes zoster patients to relieve associated pain. Good effeet was obtained in 13 patients. They experienced relief of their pain for 1-4 hours after 20 minutes of application. TNS has many advantages over analgesic drugs in the control of pain in herpes zoster.
Analgesics
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Employment
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Herpes Zoster*
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Humans
4.Telomerase Activity in Cervical Cancer.
Kyung Ah LEE ; Tae Kee JANG ; Young Jin JANG ; Young Gi LEE ; Doo Jin LEE ; Sung Ho LEE
Korean Journal of Obstetrics and Gynecology 2000;43(2):260-267
OBJECTIVE: Telomerase is a ribonucleoprotein that synthesizes TTAGGG repeats onto chromosome ends. The expression of telomerase is thought to be required for cellular immortality and carcinogenesis. This study was conducted to examine the telomerase activation occurs in cervical carcinogenesis. METHODS: The standard telomeric repeat amplification protocol(TRAP) was used to examine telomerase activity in tissues of 10 normal cervix, 10 carcinoma in situ, and 21 invasive cervical carcinoma. RESULTS: Telomerase activity was detected in tissues of 16/21(76.2%) invasive carcinoma, in 5/10(50.0%) carcinoma in situ, and in 3/10(30.0%) normal cervix. But the degree of telomerase activity in normal cervix was weak. There was significant difference in 3 groups(p<0.05). The results of neoadjuvant chemotherapy in 10 invasive cervical carcinoma were as follows. In 8 cases of which tumor size decreased more than 50%, 5 were positive for telomerase. In 2 cases that didn't respond to chemotherapy by tumor size, 1 was positive for telomerase. There was no significant difference between 2 groups. All of the 5 cases that had pelvic lymph node metastasis revealed positive telomerase activity, and the 11 cases of 16 cases that didn't have pelvic lymph node metastasis were positive for telomerase, but there was no significant difference in 2 groups. The positivity of telomerase activity in clinical stage of invasive cervical carcinoma was 73.3% in stage I(11/15), 75.0% in stage II(3/4), 100% in stage III(1/1), and 100% in stage IV(1/1), but there was no significant difference in each stages. CONCLUSION: Telomerase seems to be uniquely associated with malignant transformation of cervix and can be used as a tumor marker. Additional studies are needed to better clarify the biological significance of telomerase expression in cervical tumorigenesis.
Carcinogenesis
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Carcinoma in Situ
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Cervix Uteri
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Drug Therapy
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Female
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Lymph Nodes
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Neoplasm Metastasis
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Ribonucleoproteins
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Telomerase*
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Uterine Cervical Neoplasms*
5.Hemodynamic Responses to Dobutamine, Hydralazine and Sodium Nitroprusside Following Pentastarch Infusion during Cardiac Tamponade in Dogs.
Jin Mo KIM ; Jung Ho LEE ; Young Ho JANG ; Jae Kyu CHEUN
Korean Journal of Anesthesiology 1998;35(5):852-860
Background: Cardiac tamponade results in a hemodynamic disorder associated with decreased cardiac output and blood pressure. To improve cardiac output in a subject with cardiac tamponade, cardiotonic drugs and vasodilators with blood volume expander can be used. The purpose of this study was to observe the hemodynamic effects of cardiotonic drugs and vasodilators following administration of plasma expander in the dogs with cardiac tamponade. Method: Three groups of dogs were studied during the induced cardiac tamponade. Following infusion of pentastarch, group I received dobutamine by dripping of 10 microg/kg/min, followed by injection of 20 microg/kg/min, group II received hydralazine (20 mg, 40 mg) and group III received sodium nitroprusside (5 microg/kg/min, 10 microg/kg/min). The heart rate, blood pressure, cardiac output and pulmonary arterial occluded pressure were measured. The atrial transmural pressure was calculated by subtracting intrapericardial pressure from mean atrial pressure. Results: Cardiac output was increased in the groups I and II, but mean arterial pressure was increased in only the group I. Atrial transmural pressure was not changed in all three groups. Conclusion: The most pronounced hemodynamic improvements during the cardiac tamponade is observed in group I with pentastarch-dobutamine combination.
Animals
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Arterial Pressure
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Atrial Pressure
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Blood Pressure
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Blood Volume
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Cardiac Output
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Cardiac Tamponade*
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Cardiotonic Agents
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Dobutamine*
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Dogs*
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Heart Rate
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Hemodynamics*
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Hydralazine*
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Hydroxyethyl Starch Derivatives*
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Nitroprusside*
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Plasma
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Vasodilator Agents
6.THE POSTOPERATIVE ANALYSIS OF CLOSED REDUCTED NASAL BONE FRACTURE.
Jang Ho KIM ; Sang Hyun PARK ; Han Ho CHU ; Jin Whan CHO ; Yoo Hyun BANG
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1997;24(6):1343-1351
No abstract available.
Nasal Bone*
7.Two Cases of Clear Cell Carcinoma of Ovary.
Chang Jin JEONG ; Ki Hong CHANG ; Yoon Ho LEE ; Jang Ho LEE
Korean Journal of Gynecologic Oncology and Colposcopy 1993;4(3):86-91
Clear cell carcinoma of ovary is a rare epithelial ovarian tumor, and increased in its incidence recently. Clear cell carcinoma of ovary was known t,o be highly malignant than other epithelial ovarian tumors. The clinical and pathologic findings of two casea af clear cell carcinoma of ovary are reported and reviewed briefly.
Female
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Incidence
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Ovary*
8.The problems of L
Dong Bai SHIN ; Jang Yeub AHN ; Gung Ho JIN ; Byung Kuk CHO ; Yeon Ho KIM
The Journal of the Korean Orthopaedic Association 1995;30(4):954-959
Owing to the advancement of imaging techniques which include the CT scan, it became easier to evaluate fracture patterns of calcaneal fractures accurately. Moreover, it is possible to obtain good results with operative treatment as a consequence of the development of good operative equipment and new operative technique. In 1988, Regazzoni and Benirschke in 1990, recommended L-shaped extensive lateral approach for calcaneus which provide extensive exposure of calcaneus and so allow easier reduction and fixation. We carried out L-shaped extensive lateral approach in 11 cases from June, 1993 to April, 1994. This approach did not produce any skin problems and allowed excellent anatomical reduction and fixation. But we experienced some severe causalgia on the heel region in several cases. We tried to analyse the cause of pain and concluded that it was the damage to the lateral calcaneal branch of the sural nerve. We are reporting the problems of tbis approach.
Calcaneus
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Causalgia
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Heel
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Skin
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Sural Nerve
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Tomography, X-Ray Computed
9.Effect of the Isolation Method of Mouse Inner Cell Mass, Types of Feeder Cells and Treatment Time of Mitomycin C on the Formation Rate of ICM Colony.
Ho Jin JANG ; Kyung Rae KO ; Mi Kyung KIM ; Yong Jin NA ; Kyu Sup LEE
Korean Journal of Fertility and Sterility 2006;33(4):265-272
OBJECTIVE: This study was carried out to evaluate the effect of the isolation methods of inner cell mass from mouse blastocyst, types of feeder cells and treatment time of mitomycin C on the formation rate of ICM colony. METHODS: The inner cells were isolated by conventional immunosurgery, partial trophoblast dissection with syringe needles and whole blastocyst co-culture method. Commercially available STO and primary cultured mouse embryonic fibroblast (pMEF) feeder cells were used, and mitomycin C was treated for 1, 2 or 3 hours, respectively. The formation rate of ICM colony was observed after isolation of ICM and culture of ICM on the feeder cells for 7 days. RESULT: The ICM colony formation rate on STO were significantly higher in partial trophoblast dissection group (58%) than that in immunosurgery (12%) or whole blastocyst culture (16%) group (p<0.05). The formation rate on pMEF feeder layer was higher in partial trophoblast dissection (88%) and whole blastocyst culture (82%) group than that in immunosurgery (16%) group (p<0.05). When mitomycin C treated to pMEF for 2 hours, the formation rate of 88% was significantly higher than those of other conditions. CONCLUSIONS: Above results showed that the efficient isolation method of ICM from blastocyst was the partial trophoblast dissection and the appropriate treatment time of mitomycin C was 2 hours. However, the subculture of ICM colony and characterization of stem cells should be carried out to confirm the efficacy of the partial trophoblast dissection method.
Animals
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Blastocyst
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Coculture Techniques
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Feeder Cells*
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Fibroblasts
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Mice*
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Mitomycin*
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Needles
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Stem Cells
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Syringes
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Trophoblasts
10.Comparative Quantitative Study of Surfactant Protein C mRNA by Filter Hybridization and Solution Hybridization in Rats.
Jin Ho KIM ; Jang Won SOHN ; Seok Chul YANG ; Ho Joo YOON ; Dong Ho SHIN ; Sung Soo PARK
Tuberculosis and Respiratory Diseases 2001;51(6):517-529
BACKGROUND: Surfactant protein C(SP-C) is a hydrophobic 5,000 dalton molecule. SP-C has the primary roles in accelerating surface spreading of a surfactant phospholipid. The filter hypbridization and solution hybridization assays are both rapid and sensitive and can be used to measure the RNAs complementary to any cloned DNA sequence. METHODS: The authors measured the SP-C mRNA levels quantitatively using solution hybridization and filter hybridization assays to obtain a standard curve equation to quantify the mRNA of unknown samples comparatively. RESULTS: 1. The minimum level of the specimens by solution hybridization was 3 pg for SP-C mRNA. 2. The standard curve equation of the solution hybridization assay between the counts per minute(Y) and the SP-C mRNA transcript input(X) was Y=6.46 X+244. The correlation coefficient was 0.9. 3. The minimum detection level of specimens by filter hybridization was 0.1 ng for SP-C mRNA. 4. The standard curve equation of the filter hybridization assay between the counts per minute(Y) and SP-C mRNA transcript input(X) is Y=2541.6 X+252.7. The correlation coefficient was 0.99. CONCLUSION: A comparison of CPM/filter in the linear range allowed an accurate and reproducible estimation of the SP-C mRNA copy number. Filter hybridization and solution hybridization assays are both rapid and sensitive and can be used to measure the RNAs complementary to any cloned DNA sequence. It is ideally suited to situations where accurate quantitation of multiple samples is required.
Animals
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Base Sequence
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Clone Cells
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Protein C*
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Rats*
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RNA
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RNA, Messenger*