BACKGROUND: Long-term oral itraconazole and terbinafine are widely used in the treatment of onychomycosis. Accelerated nail growth in patients with itraconazole has been described in several reports. However, there has been no report regarding the effect of antifungal agents on cultured nail matrix cells(NMCs). OBJECTIVE: We applied several antifungal agents on cultured human NMCs and epidermal kera- tinocytes(EKs) to compare the cytotoxicity of several antifungal agents and also to verify possible stimulating effects of itraconazole and 6-hydroxyitraconazole on nail growth. METHODS: To evaluate the effect of antifungal agents, the 3-(4,5-dimethylthiazo1-2-yl) 2,5-diphenyl- tetrazolium bromide(MTT) test, tritiated thymidine incorporation test, and lactic dehydrogenase(LDH) leakage test were used. RESULTS: Dose dependent decreases in cell viability and DNA synthesis, and dose dependent increases in LDH liberation were observed in cultured human NMCs and EKs after exposure to several antifungal agents. The dose-response reaction patterns for NMCs and EKs to antifungal agents were similar. The cytotoxicity potency of several antifungal agents measured by each method were slightly different. Itraconazole and 6-hydroxyitraconazole did not show stimulating effects on cell proliferation in in vitro monolayer cell culture systems. CONCLUSION: These observations suggest that itraconazole appeared less cytotoxic but showed no stimulating effects on nail matrix cell proliferation in vitro. Cultured human EKs as well as NMCs may be useful in evaluating the effects of agents which are involved in nails.
Antifungal Agents* ; Cell Culture Techniques ; Cell Proliferation ; Cell Survival ; DNA ; Humans* ; Itraconazole ; Keratinocytes* ; Onychomycosis ; Thymidine

No abstract available.

No abstract available.

Echocardiography was done with Ekoline 20 model of Smith Kline Instrument in 91 patients of mitral valvular heart disease, 46 with mitral stenosis, 10 with mitral insufficiency, 38 with mitral stenoinsufficinency and 115 normal controls. The results obtained are as follows. 1. Right ventricular dimension was 13.61+/-5.61 mm/sq m. in normal controls, 16.50+/-5.06mm/sq m. in mitral stenosis, 20.17+/-3.28mm/sq m. in mitral insufficiency, 18.61+/-8.48mm/sq m. in mitral stenoinsufficiency revealing significant increment in all mitral valvular disease groups but there was no significant difference btw. each disease groups. 2. Left ventricular end-diastolic dimensions was 29.83+/-7.06mm/sq m. in normal controls, 41.57+/-7.77mm/sq m. in mitral insufficinency, 38.48+/-10.22mm/sq m. in mitral stenoinsufficiency revealing significant increment but there was no significant difference btw. mitral stenosis and normal controls. 3. Left atrial dimension was 17.57+/-4.96mm/sq m. in normal controls, 33.22+/-5.32mm/sq m. in mitral stenosis, 35.96+/-7.35mm/sq m. in mitral insufficiency, 35.00+/-8.22 in mitral stenoinsufficiency revealing significnat increment in whole mitral valvular disease. 4. E-F slope was 90.76+/-30.20mm/sec in normal controls 18.08+/-10.3mm/sec in mitral stenosis, 29.52+/-19.3mm/sec in mitral stenoinsufficiency revealing marked decrement. In mitral insufficiency, it was 65.3+/-45.4mm/sec revealing significant decrement but not so severe as previous. 5. There was no significant difference between normal controls and mitral valvular disease in valve excursion. 6. Left atrial dimension to aortic root size was 1.13+/-0.31 in normal controls, 1.77+/-0.63 in mitral stenosis, 1.56+/-0.60 in mitral regurgitation and 1.82+/-0.56 in mitral stenoinsufficinecy revealing significant increment in whole mitral valvular heart disease.
Echocardiography* ; Heart Valve Diseases* ; Humans ; Mitral Valve Insufficiency ; Mitral Valve Stenosis

Epidermodysplasia vertuciformis (E.V.) is a generalized viral infection due to the wart virus, which is chacterized by wartlike papules often distributed over the entire body and generally starting in infancy, of which a high percetage develops into cancer. We present a case of E.V. in a 31 year old male with typical skin lesions resembling flat warts and tinea versicolor-like lesions that eventuated in malignancy, i.e., Bowens disease and squamous cell carcinoma on the forehead. The skin lesions appeared initially on the foerhead at the age of 6 and gradualIy generalized all over the whole bcdy thereafter. The light brown scaling papules on erythernatous hase and walnut sized ulcerative lesion on the forehead had been noticed for 4 to 6 years and identified as Bowens disease and squamous cell carcinoma.
Adult ; Bowen's Disease* ; Carcinoma, Squamous Cell ; Epidermodysplasia Verruciformis* ; Forehead ; Humans ; Juglans ; Male ; Skin ; Tinea ; Ulcer ; Warts

The authors reviewed retrospectively retinal detachment(RD)s following posterior chamber intraocular lens(PC-IOL) implantation by transscleral suture fixation. One hundred twenty-two transscleral fixations of PC-IOL were performed from January, 1990 to December, 1992. The follow-up periods for this series ranged from 12 to 42 months. Six retinal detachments developed in this series. and the incidence of RD was 4.9%. There was no statistical difference between the primary operation group(unplanned posterior capsule rupture during extracapsular cataract extraction, lens dislocation/subluxation) and the secondary operation group(already aphakia after previous cataract surgery). The predisposing factors to RD were myopic eye(p=0.03), postoperative vitreous hemorrhage(p=0.001). In cases with RD, the locations of transscleral fixation sutures almost coincided with the meridians of the retinal breaks. The contributing factors to increased incidence of RD were thought to be vitreous loss during operations, vitreous traction by suturing needle or haptics of IOL, proliferative change by vitreous hemorrhage.
Aphakia ; Cataract ; Cataract Extraction ; Causality ; Follow-Up Studies ; Incidence ; Lenses, Intraocular* ; Meridians ; Needles ; Retinal Detachment* ; Retinal Perforations ; Retinaldehyde* ; Retrospective Studies ; Rupture ; Sutures ; Traction ; Vitreous Hemorrhage

No abstract available.
Pancreatic Pseudocyst*

No abstract available.
Anemia, Hemolytic* ; Child* ; Humans

No abstract available.
Neurofibromatoses* ; Scoliosis*

Twenty nine cases of wrist tuberculosis were reviewed(19 joint involvement cases and 4 tendon involvement cases) and twenty three cases were followed up more than one and half years. The mean follow up period was 26.7 months. Synovectomies were not satisfactory in cases of joint involvement and they were useful only in cases of tendon involvement. Arthrodesises were used for 16 cases of joint involvement and were satisfactory in 87,5 percent by Robin's criteria. Finger stiffness was most serious complication(initially, 8.7 percent and finally, 34.8 percent) and it is suggested that early motion of fingers after arthrodesis can prevent this complication.
Arthrodesis ; Fingers ; Follow-Up Studies ; Joints ; Tendons ; Tuberculosis ; Wrist