1.The Antiproliferative and Redifferentiative Effects of Na-4-Phenylbutyrate in Human Thyroid Cancer Cell Lines.
Young Jin CHOI ; Jin Woo PARK ; Lee Chan JANG ; Jae Woon CHOI ; Orlo H CLARK
Journal of the Korean Surgical Society 2008;75(3):162-170
PURPOSE: Sodium-4-phenylbutyrate (Na-4-PB) is an analogue of phenylacetate, which is a well-known redifferentiating agent. In vitro and in vivo studies on this agent have been done and the clinical relevance of Na-4-PB has been studied in other malignancies, but not in thyroid cancer. We investigated the effect of Na-4-PB on cell proliferation and differentiation in thyroid cancer cell lines. METHODS: We used 5 thyroid cancer cell lines: TPC-1, FTC-133, FTC-236, FTC-238 and XTC-1. MTT assay and flowcytometry were used to measure the agent's antiproliferative effects and the cell cycle change. We evaluated the PPARgamma expression via western blotting and the mRNA expressions of NIS, Tg and CD 97 were determined by performing RT-PCR. Troglitazone, a potent PPARgamma agonist, was used in combined treatment with Na-4-PB. RESULTS: Na-4-PB inhibited cell proliferation in a dose and time dependent manner in all 5 thyroid cancer cell lines. By performing flowcytometry in the FTC-133 and TPC-1 cell lines, we identified that the antiproliferative effect of Na-4-PB was associated with an increased apoptotic cell population. Treatment with Na-4-PB upregulated the PPARgamma expression, but the combined treatment of Na-4-PB with troglitazone did not seem to be synergistic for the antiproliferative effect. Treatment with Na-4-PB downregulated the CD97 mRNA expression and it upregulated the NIS and Tg mRNA expressions in both the FTC-133 and TPC-1 cell lines. CONCLUSION: Na-4-PB inhibited thyroid cancer cell proliferation by inducing apoptosis in a dose dependent manner. Treatment with Na-4-PB increased the expression of PPARgamma and it upregulated such differentiation markers as NIS and Tg, and it downregulated CD97, a dedifferentiation marker. Na-4-PB should be further evaluated as a new potential therapeutic agent for patients with thyroid cancer.
Antigens, Differentiation
;
Apoptosis
;
Blotting, Western
;
Cell Cycle
;
Cell Line
;
Cell Proliferation
;
Chromans
;
Histone Deacetylase Inhibitors
;
Humans
;
Phenylacetates
;
PPAR gamma
;
RNA, Messenger
;
Thiazolidinediones
;
Thyroid Gland
;
Thyroid Neoplasms
2.A case of neonatal alloimmune thrombocytopenia due to anti-HLA B44.
Kyou Sup HAN ; Myoung Hee PARK ; Bok Yun HAN ; Jung Hye CHOI ; Jin Min CHOI ; Hwa Ryung CHUNG ; Richard H ASTER
Korean Journal of Blood Transfusion 1993;4(2):239-245
No abstract available.
Thrombocytopenia, Neonatal Alloimmune*
3.A case of neonatal alloimmune thrombocytopenia due to anti-HLA B44.
Kyou Sup HAN ; Myoung Hee PARK ; Bok Yun HAN ; Jung Hye CHOI ; Jin Min CHOI ; Hwa Ryung CHUNG ; Richard H ASTER
Korean Journal of Blood Transfusion 1993;4(2):239-245
No abstract available.
Thrombocytopenia, Neonatal Alloimmune*
4.A retrospective comparison of four different procedures for extracting dermoid cyst by laparoscopy.
Yoon S LEE ; Taek H LEE ; Young R CHO ; Sang S CHUN ; Il S PARK ; In K KANG ; Tae B KOO ; Jin H CHOI
Korean Journal of Obstetrics and Gynecology 2002;45(2):239-243
OBJECTIVES: To compare results of 4 different extraction methods in laparoscopic management of dermoid cyst. STUDY DESIGN: This article is a retrospective, multicenter study for 247 patients with benign dermoid cyst in period of 1995-1998. Dermoid cyst was extracted by Endopouch (99 cases), puncture-irrigation-extraction (69 cases), colpotomy (35 cases), and dermoid cyst as a "pouch bag" (44 cases). RESULTS: We analyzed irrigation amount, operative time, postoperative hospital stay and complications by four different extraction methods. Endopouch extraction method needed less amount of irrigation fluid for cleaning the abdominal cavity and had a shorter postoperative hospital stay (ANOVA, p=0.0001). There were no significant differences in operative times among groups. There were four cases of morbidity in puncture-irrigation-extraction method (6%), three had fever (> 38degrees C) and one intraabdominal abscess. One incisional hernia was noted in "pouch bag" method (2%). CONCLUSIONS: We recommend minimal spillage method for extraction of dermoid and careful irrigation of abdominal cavity to prevent potential risk of chemical peritonitis such as Endopouch, "pouch bag", and colpotomy with the exception of puncture-irrigation-extraction method.
Abdominal Cavity
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Abscess
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Colpotomy
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Dermoid Cyst*
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Fever
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Hernia
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Humans
;
Laparoscopy*
;
Length of Stay
;
Operative Time
;
Peritonitis
;
Retrospective Studies*
5.Value of MR Imaging after CT in Patients with Focal Hepatic Lesion.
Kyeong Ah KIM ; Jae Hoon LIM ; Sang Hee CHOI ; Soon Jin LEE ; Chul H PAIK ; Jae Min CHO
Journal of the Korean Radiological Society 1999;40(5):915-920
PURPOSE: To determine the usefulness of magnetic resonance imaging (MRI) after computed tomography (CT) inpatients with focal hepatic lesion. MATERIALS AND METHODS: We evaluated 100 patients with 103 focal hepaticlesions. The diagnosis of each lesion was made pathologically (n=19), or radiologically and clinically (n=84), andthe findings were as follows: he-mangioma (n=53), hepatocellular carcinoma (n=17), metastasis (n=10), cyst (n=5),regenerative nodule (n=3), and adenomatous hyperplasia (n=3). The patients underwent conventional CT (n=25),two-phase spi-ral CT (n=17) or three-phase spiral CT (n=61). MRI was performed using conventional T1- andT2-weighted imaging and dynamic contrast enhancement. The value of MRI after CT was assigned to one of fourgrades, according to the consensus of three radiologists: grade I (decisive), grade II (helpful), grade III (notadditional), or grade IV (confused). RESULTS: The outcome of MRI of 103 lesions was grade I in 14 cases(14%), IIin 34 (33%), III in 49 (48%), and IV in 6 (6%). MRI was not helpful (grade III or IV) in 40% (10/25), 47% (8/17),and 61%(31/61) of lesions after conventional, two-phase spiral, and three-phase spiral CT, respectively. Grade IIIor IV lesions were present in 45% of hemangiomas (24/53), 59% of hepatocellular carcinomas (10/17), and 80% ofcases in which metastasis had occurred(8/10). CONCLUSION: MRI after CT in patients with focal hepatic lesion washelpful in less than half of all cases. It was particularly valuable for patients who did not undergo three-phasespiral CT and in whom hemangioma was suspected.
Carcinoma, Hepatocellular
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Consensus
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Diagnosis
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Hemangioma
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Humans
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Hyperplasia
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Inpatients
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Magnetic Resonance Imaging*
;
Neoplasm Metastasis
;
Tomography, Spiral Computed
6.Proportion of Multidrug-Resistant Tuberculosis in Human Immunodeficiency Virus/Mycobacterium tuberculosis Co-Infected Patients in Korea.
Joon Sung JOH ; H Christian HONG ; In A JEONG ; Bum Sik CHIN ; Hye Jin YANG ; Hongjo CHOI ; Ji Hwan BANG
Journal of Korean Medical Science 2012;27(10):1143-1146
Much controversy surrounds the issue of whether HIV infection is a risk factor for developing multidrug-resistant tuberculosis (MDR-TB). In this study, we evaluated the prevalence of and risk factors for MDR-TB in HIV-infected patients at the National Medical Center of Korea. We reviewed the medical records of HIV/TB co-infected patients from January 2005 to May 2011; the drug susceptibility profiles were available for 55 patients. Of these, 32.7% had MDR-TB, which was approximately 3.6 times higher than the prevalence among the general population. Additionally, there were more additional AIDS-defining clinical illnesses in the MDR-TB group than in the non-MDR-TB group (27.8% vs 5.4%, P = 0.032). These results suggest that HIV infection and HIV-related immunosuppresion may contribute to the development of MDR-TB.
Adult
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Aged
;
Coinfection
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Female
;
HIV Infections/*complications/*epidemiology/microbiology
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Humans
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Immunosuppression
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Male
;
Middle Aged
;
Mycobacterium tuberculosis/isolation & purification
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Prevalence
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Republic of Korea/epidemiology
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Retrospective Studies
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Risk Factors
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Tuberculosis, Multidrug-Resistant/*complications/*epidemiology/microbiology
7.Cyclopamine Inhibits Cancer Cell Proliferation in Thyroid Cancer Cell Lines.
Sung Su PARK ; Jin Woo PARK ; Jae Woon CHOI ; Lee Chan JANG ; Sung Il WOO ; Young Jin CHOI ; Orlo H. CLARK
Korean Journal of Endocrine Surgery 2007;7(2):69-74
PURPOSE: The Hedgehog (HH) signaling pathway is important in development. Recently,ectopic activation of this pathway has been implicated in several forms of solid cancer including basal cell carcinoma, pancreatic cancer, colon cancer, and prostate cancer. There are three HH proteins involved in the pathway: Sonic HH, Indiana HH, and Desert HH. Cyclopamine disrupts Sonic HH signaling by inhibition of the seven-transmembrane receptor Smoothened (SMO). Whereas cyclopamine is cytotoxic to several human cancer cells, its effect on thyroid cancer cellsis unknown. We therefore investigated the effect of cyclopamine on cell proliferation in human thyroid cancer cell lines. METHODS: We used fivethyroid cancer cell lines: TPC-1 (papillary), FTC-133, FTC-236, FTC-238 (follicular), and XTC-1 (Hurthle cell). The MTT assay and cell cycle analysis were used to evaluate anti-proliferative effects. Tomatidine, a structural analogue of cyclopamine, was used as a control agent. Statistical significance was tested by ANOVA. RESULTS: After 4 days of treatment, the percent inhibition of growth with a concentration of 5, 10, and 20 M cyclopamine in the cell lines were 23.6±4.9%, 66.4±4.7% and 69.3±1.3% in TPC-1 7.5±2.8%, 10.7±3.2% and 49.6±6.4% in FTC-133, 19.2±9.5%, 50.4±4.8% and 60.4±2.0% in FTC- 236 22.8±4.2%, 53.4±5.5% and 63.7±4.8% in FTC- 238 7.6±5.8%, 16.6±2.2%, 24.0±4.3% in XTC-1. Treatment with tomatidine at the same concentrations did not significantly affect cell growth. Exposure to cyclopamine, however, did not affect the cell cycle significantly CONCLUSION: Cyclopamine inhibits cancer cell proliferation in a dose dependent manner in thyroid cancer cell lines. The Hh signaling pathway might be a useful therapeutic target for thyroid cancer.
Carcinoma, Basal Cell
;
Cell Cycle
;
Cell Line*
;
Cell Proliferation*
;
Colonic Neoplasms
;
Hedgehogs
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Humans
;
Indiana
;
Pancreatic Neoplasms
;
Prostatic Neoplasms
;
Thyroid Gland*
;
Thyroid Neoplasms*
8.Pilot study for the Psychometric Validation of the Sheffield Profile for Assessment and Referral to Care (SPARC) in Korean Cancer Patients
Jung Hye KWON ; Sun Kyung BAEK ; Do Yeun KIM ; Yu Jung KIM ; Myung Ah LEE ; Hye Jin CHOI ; Ja Min BYUN ; Jin Young JEONG ; Sam H AHMEDZAI ; Geun-Doo JANG
Cancer Research and Treatment 2021;53(1):25-31
Purpose:
This study aimed to validate the Sheffield Profile for Assessment and Referral to Care (SPARC) as an effective tool for screening palliative care needs among Korean cancer patients.
Materials and Methods:
The English version of the SPARC was translated by four Korean oncologists and reconciled by a Korean language specialist and a medical oncologist fluent in English. After the first version of the Korean SPARC (K-SPARC) was developed, back-translation into English was performed by a professional translator and bilingual oncologist. The back-translated version was reviewed by the original author (S.H.A.), and modifications were made (ver. 2). The second version of the K-SPARC was tested against other questionnaires, including the Functional Assessment of Cancer Therapy-General (FACT-G) and the Edmonton Symptom Assessment System (ESAS).
Results:
Thirty patients were enrolled in the pilot trial. Fifteen were male, and the median age was 64.5 years. Six patients had an Eastern Cooperative Oncology Group performance status of 2 or more. All patients except one were receiving chemotherapy. Regarding internal consistency, the Cronbach’s α scores for physical symptoms, psychological issues, religious and spiritual issues, independency and activity, family and social issues, and treatment issues were 0.812, 0.804, 0.589, 0.843, 0.754, and 0.822, respectively. The correlation coefficients between the SPARC and FACT-G were 0.479 (p=0.007) for the physical domain and –0.130 (p=0.493) for the social domain.
Conclusion
This pilot study indicates that the K-SPARC could be a reliable tool to screen for palliative care needs among Korean cancer patients. A further study to validate our findings is ongoing.
9.Lung Cancer Screening with Low-Dose Helical CT in Korea: Experiences at the Samsung Medical Center.
Semin CHONG ; Kyung Soo LEE ; Myung Jin CHUNG ; Tae Sung KIM ; Hojoong KIM ; O Jung KWON ; Yoon Ho CHOI ; Chong H RHEE
Journal of Korean Medical Science 2005;20(3):402-408
To determine overall detection rates of lung cancer by low-dose CT (LDCT) screening and to compare histopathologic and imaging differences of detected cancers between high- and low-risk groups, this study included 6,406 asymptomatic Korean adults with >or=45 yr of age who underwent LDCT for lung cancer screening. All were classified into high- (>or=20 pack-year smoking; 3,353) and low-risk (3,053; <20 pack-yr smoking and non-smokers) groups. We compared CT findings of detected cancers and detection rates between high- and low-risk. At initial CT, 35% (2,255 of 6,406) had at least one or more non-calcified nodule. Lung cancer detection rates were 0.36% (23 of 6,406). Twenty-one non-small cell lung cancers appeared as solid (n=14) or ground-glass opacity (GGO) (n=7) nodules. Cancer likelihood was higher in GGO nodules than in solid nodules (p<0.01). Fifteen of 23 cancers occurred in high-risk group and 8 in low-risk group (p=0.215). Therefore, LDCT screening help detect early stage of lung cancer in asymptomatic Korean population with detection rate of 0.36% on a population basis and may be useful for discovering early lung cancer in low-risk group as well as in high-risk group.
Adenocarcinoma/diagnosis
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Aged
;
Aged, 80 and over
;
Carcinoma, Non-Small-Cell Lung/diagnosis
;
Carcinoma, Squamous Cell/diagnosis
;
Comparative Study
;
Female
;
Humans
;
Korea
;
Lung Neoplasms/*diagnosis
;
Male
;
Mass Screening/*methods
;
Middle Aged
;
Risk Factors
;
Tomography, X-Ray Computed/*methods
10.Reliability and Validity of the Short Form of the Literacy-Independent Cognitive Assessment in the Elderly.
Jungeun KIM ; Jee H JEONG ; Seol Heui HAN ; Hui Jin RYU ; Jun Young LEE ; Seung Ho RYU ; Dong Woo LEE ; Yong S SHIM ; Seong Hye CHOI
Journal of Clinical Neurology 2013;9(2):111-117
BACKGROUND AND PURPOSE: The Literacy-Independent Cognitive Assessment (LICA) has been developed for a diagnosis of dementia and is a useful neuropsychological test battery for illiterate populations as well as literate populations. The objective of this study was to develop the short form of the LICA (S-LICA) and to evaluate the reliability and validity of the S-LICA. METHODS: The subtests of the S-LICA were selected based on the factor analysis and validation study results of the LICA. Patients with dementia (n=101) and normal elderly controls (n=185) participated in this study. RESULTS: Cronbach's coefficient alpha of the S-LICA was 0.92 for illiterate subjects and 0.94 for literate subjects, and the item-total correlation ranged from 0.63 to 0.81 (p<.01).The test-retest reliability of the S-LICA total score was high (r=0.94, p<.001), and the subtests had high test-retest reliabilities (r=0.68-0.87, p<.01). The correlation between the K-MMSE and S-LICA total scores were substantial in both the illiterate subjects (r=0.837, p<.001) and the literate subjects(r=0.802, p<.001). The correlation between the S-LICA and LICA was very high (r=0.989, p<.001). The area under the curve of the receiver operating characteristic was 0.999 for the literate subjects and 0.985 for the illiterate subjects. The sensitivity and specificity of the S-LICA for a diagnosis of dementia were 97% and 96% at the cutoff point of 72 for the literate subjects, and 96% and 93% at the cutoff point of 68 for the illiterate subjects, respectively. CONCLUSIONS: Our results indicate that the S-LICA is a reliable and valid instrument for quick evaluation of patients with dementia in both illiterate and literate elderly populations.
Aged
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Dementia
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Literacy
;
Humans
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Neuropsychological Tests
;
Reproducibility of Results
;
ROC Curve
;
Sensitivity and Specificity