0.1 mv.The rabbits were randomly divided into two groups(n=16):group I was the control;in group Ⅱ, 1% lidocaine 2～3 mg/kg was infused to epidural space.Hemodynamics such as MAP,HR,left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP) and ?dp/dt max were monitored pre- and 30min post-ligation.The extent of infarct area was determined by TTC staining.Results:After ligation,LVEDP increased and LVSP、?dp/dt max decreased in the control group.TEA significantly repressed the increasing of LVEDP and the decreasing of LVSP,?dp/dt max caused by LAD ligation.TEA could reduce the size of infarcted area while there was no significant difference in 4h,8h after ligation.Conclusion:TEA can significantly protect myocardium from injury in rabbits with experimental myocardial infarction.

Animal tumor models, as replicas of human tumors, are of important significance concerning the investigation of the mechanism of tumorigenesis, tumor progression, tumor prevention and therapy, when establishing animal tumor models, we should choose suitable species of animals and corresponding carcinogens. One species of animal differs greatly from others. The same carcinogen may induce different tumor in different species of animals. Thus it is most important that animals should be selected properly to obtain animal tumor models suitable for experiment. Animal tumor models comprise spontaneons tumor models, inducing tumor models and transplantable tumor models. This paper will focus on the transplantable animal tumor models. The sources of human tumors (transplanted to immunodeficiency animals) are mainly biopsy tissues, surgically resected tumor specimens and human tumor cell lines. The basic necessity to establish transplantable tumors includes collecting fresh, non-necrotic non-capsulated tumor tissue within 1-2 hours after resection, selecting host animal (including immunodeficiency animals) should be around 4 weeks old, and the most frequent innoculating site is dorsal subcutaneous. Successful establishment of transplantable tumor models should satisfy the following standards: 15 - 20 successive generations (at least 3 - 4 animals each generation); 100 % growth after transplantation; least self-extinction (not definitely zero); stable growth rate; similar life span of host (high reproductivity); low host response (suitable for in vivo growth in host); histological similarity with primary tumor.

With the rapid development of science and the education modernization project,the computer technology being introduced in function experiment teaching process is the significant measure in medical education reform.With the aid of the computer technology and the network technology,we can realize the medical education information teaching pattern.The computer will play the pivotal role either in the basic medicine teaching or in the experimental teaching,.

Results of observation on the relationship between the different implantation site and metastasis of cancer cells were reported. In the group of intramuscular implantation, lymphatic metastasis was 100%, lung metastasis also was 100% and metastasis in the kidney exhibited 26%. In the group of footpad inoculation, the lung metastasis was 84%, lymphatic metastasis presented 100%, kidney metastasis only exhibited 7%. in the group of intravenous inoculation, lung metastasis was 88%, itsr forms of metastasis most exhibited large nodules; lymphatic metastasis presented 74%; the large metastatic tumors were found in the gluteal part, dosal part, abdominal wall and some parts of four limbs. The survival time of tumor bearing mice of above three groups was more than 20 days. In the group of postocular venous plexus inoculation, lung metastasis was 60%, lymphatic metastasis was 65% In the group of intraspleenic inoculation, lung and liver metastases were 44% respectively. No metastasis was observed in lymph nodes. The survival time of tumor-bearing mice of latter two groups was in 7—14 days. Our results demonstrate the influence of the site of transplanted tumor on the formation of metastasis. The relationship between the site of transplanted and metastasis as well as metastatic mechanism are discussed.

0bjective:To compared the efficacy and safety of lornoxicam combined with tramadol used in patient-controlled analgesia (PCA) in patients undergoing renal transplatation. Methods:60 patients renal transplatation were randomly divided into Buprenorphine combined with tramadol group(I group) and lornoxicam combined with tramadol group(Ⅱ group). The PCA pump was started according to the need of patients.The efficacy was evaluated by both PAR(pain relief) and TOTPAR(total pain relief),coagulation、transplatation renal survival rate and adverse reactions were also observed.Results:No significant statistical difference in both PAR and TOTPAR as well as renal function were observed between the 2groups,but lornoxicam has less adverse effects.Conclusion:The analgesia effect of Ⅱ group used in PCA for renal transplatation comparable to that of I group but Ⅱ group with less adverse effects.

Objective:To summarize the differences and similarities in the detection methods for residual solvents between Safety and Technical Standards for Cosmetics 2015 edition and Chinese Pharmacopoeia 2015 edition so as to provide reference for the improve-ment of the detection methods for residual solvents in Safety and Technical Standards for Cosmetics. Methods:The type and limitations of residual solvents and the characteristics of the test methods for residual solvents between Safety and Technical Standards for Cosmetics 2015 edition and Chinese Pharmacopoeia 2015 edition were compared and analyzed. Results: The detection methods for residual sol-vents in Chinese Pharmacopoeia were more detailed. The detection methods for residual solvents in Safety and Technical Standards for Cosmetics were general detection method, and the process could be applied in the detection of more solvents. Some detection methods were short of limitations. Conclusion:The control of residual solvents in cosmetics should be improved if the limitations table of the limiting used solvents is introduced into Safety and Technical Standards for Cosmetics referring to Chinese Pharmacopoeia and increase the types of residual solvents detected by the general methods.

OBJECTIVE: To study the effect of samples having different pH values in the bacterial endotoxins test (BET). METHODS: The BET was conducted as an assay of the concentration of endotoxins by using kinetic-turbidimetric technique. Samples having different pH values but containing same concentration reference standard endotoxin (RSE) were tested by TAL/LAL reagents. The average result on the standard curve was calculated and the mean recovery observed. RESULTS: The reagents had three highly sensitivities in pH 5.20, pH 7.83/pH 6.23 and pH 10.55. After adjustment to pH 6 to 8, the mean recovery was 76.5%～115.9% or 85.8%～99.8%. When the sample pH value was less than 3 or more than 12, the test was unsuitable for the inhibition test, and the mean recovery was less than 56.8%. CONCLUSION: It is necessary to adjust the pH of the solution to be tested in the BET, adjust the sample to pH (6.5～7.5) when using TAL reagents, and adjust to pH (6.2±0.1) or pH(6.7～7.8) when using LAL reagents.

Objective To optimize multiplicity of infection ( MOI) and antibiotics ( blasticidin) concentration selecting BSD gene in construction of monoclonal stable cell line by lentivirus vector-mediated RNA interence silenced gene SGMS2.Methods The INS-1 cells were transfected by fluorescence labeled negative control SGMS2-siRNA lentivirus at MOI of 0, 10, 30, 60 and 120 TU number/cell.The cells were photographed under fluorescent microscopy after 72 h cultivation, then fluorescence ratio and apoptosis rate were calculated to determine optimal MOI.The INS-1 cells were treated by blasticidin with different concentrations of 0, 1, 2, and 3 μg/mL, and the apoptosis rate was observed to acquire optimal concentration of antibiotics.The INS-1 cells were transfected by negative control SGMS2-siRNA lentivirus and SGMS2-siRNA lentivirus (virus titer:1 ×108TU/mL) at optimal MOI and positive-transfected cells were selected by blasticidin at optimal concentration, then mixed cell lines were acquired.The monoclonal cell line was constructed at fluorescence ratio of 90%.Results The optimal MOI was 60 with 100% fluorescence ratio, less than 0.5% apoptosis rate and keep original cellular morphology.The optimal concentration of blasticidin was 2 μg/mL with cell adherence disappear and all cells apoptosis.The Ct value of INS-1-SEMS2 cells detected at the second time was 28.21, which was greater than 27.58 at the first time.The interfering efficiency of siRNA was 77.78% which indicated a successful expression of siRNA and construction of monoclonal stable cell line ( INS-1-SEMS2 ).Conclusion The monoclonal stable cell line was successfully constructed by lentivirus vector-mediated RNA interence silenced gene SGMS2.

Objective To assess the diaghosis vaIne of colposcopy in cervical disease.Methods 169 with patients colposcopyaere admitted in endoscopic biopsy.Results Colposeopy to be diagnosed as chronic cervicifis 121 cases(71.6%),cervical intraepithelial neoplasia 27 cases(16.0%),6 cases of cervical cancer(3.6%),8 cases of cervical polyps(4.7%),incisive condyloma 7 cases(4.1%).Biopsy pathology report to the chronic cervicitis 123 cases(72.8%),cervical intraepithehal neoplasia 26 cases(15.4%),6 cases of cervical cancer(3.6%),8 cases of cervical polyps(4.7%),incisive condybma 6 cases(3.6%).The sensitivity and specificity of colposcopy higher.Conclusion Colposcopy for cervical disease is similar to the result of the pathology results,Which has a very imporrant diagnostic value.Combining colposcopy and pathological diagnosis of a certain relevance,the organic integration of the two can significantly improve the accuracy of diagnosis of cervical lesions.

Myocardial tissue masses were isolated from two human fetus of 10 and 16 week's gestation respectively and they were cultured under the conditions for cultivating the myocardial cells of the rodents. Spontaneous beating occured. In one case the frequency of beating was 21～27 beats/min, and the beating lasted for 30 days until the culture was terminated artifitially. In another case a cell mass continued to beat as long as 75 days with a frequency of 200～290 beats/min, and then stopped spontaneously. The differences in thickness and shapes of the tissue masses would influence the modes of growth after culturing. Thicker tissue mass grew more slowly and the fibroblasts and myocardial cells grew and spreaded out radially, or in band form. In tissue mass with a thin border the marginal myocardial cells bulged out in globular shape in the early stage and then separated from the cell mass and continued to grow. In thin layered tissue mass, because of the rapid overgrowth of the surrounding fibroblasts, the myocardial tissue would grow and overlap them and increased steadily in thickness. This procedure is simple and relatively stable, and possible to retain the long-lasting beating of the myocardial tissus, but it is difficult to obtain a pure myocardial cell culture for cell counts and other quantitative studies.