Brucellosis ; diagnosis ; therapy ; Female ; Humans ; Malta ; Middle Aged ; Spondylitis ; diagnosis ; therapy

Owning to the progression of silence and technology, increasing techniques for in vivo cell tracking have been applied in medical research; they include radionuclide imaging, magnetic resonance imaging, optical imaging, etc. These imaging methods have their own characteristics in specificity, sensitivity and manipulation mode. Researchers should choose the appropriate method while taking the following into consideration requirement of the image, object of the image, the aim of the image, and the characteristics of each imaging method. In this paper we summarize the techniques of cell tracking in vivo.

Objective To introduce transfusion monitoring system to optimize nursing flow under field conditions.Methods The organization and arrangement of nursing staffs were optimized with high-precision droplet sensor and transfusion monitoring system.A post of caring nurse was added to execute mental healthcare of the patient.Results Humanistic care was carried out by the caring nurse with daily nursing completed,and there's no need for additional nursing staff.Conclusion The transfusion monitoring system is rapid in deployment and withdrawal,optimizes nursing flow and enhances nursing quality,and thus is worthy promoting widely.

Adolescent ; Adult ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Medicine, Chinese Traditional ; methods ; Ovarian Diseases ; physiopathology ; therapy ; Ovary ; physiopathology ; Phytotherapy ; Young Adult

Objective To explore the role of RBC[Ca2+]i levels in pathogenesis of hypoxic-ischemic encephalopathy (HIE) in neonates. Methods Twenty-eight neonates with moderate and severe HIE hospitalizeal from Jun. 2002 to Mar. 2006 were enrolled the study. The neonates with HIE were given routine treatment and Nimodipine for 7～10 days. Blood samples were collected before treatment and at 72 hours,7～10 days after treatment respectively. The levels of RBC [Ca2+]i were measured by Fura-2/AM. Twenty healthy full-term neonates were studied as controls. Results (1) The levels of RBC [Ca2+] i in the neonates with moderate and severe HIE were significantly higher than that in control group at every time points( P＜0. 05 ,P＜0.01). (2) the levels of RBC[Ca2+]i in the neonates with moderate and severe HIE peaked at 72 hours after treatment,and were still significantly higher than that of control group at 7～10 days after treatment(P＜0. 05). (3) In the neonates with HIE,RBC[Ca2+ ]i levels correlated positively with the severity of HIE ( r = 0. 447, P＜ 0. 05 ). Conclusion RBC [Ca2+ ] i levels are closely associated with pathogenesis of HIE, and may play an important role in the pathogenesis of HIE. Evaluating RBC [ Ca2+] i levels in neonate after birth may provide clinical clues for the early diagnosis and prognosis assessment of HIE.

Objective To detect the intestinal pathogenic bacteria quickly and accurately from water. Methods An experimental procedure is set up using the gene chip technology to detect and identify common intestinal pathogenic bacteria from water. Target gene was amplified and hybridized with prepared gene chip.Results 143 strains of bacteria in pure culture belong to 9 genera are successfully discriminated under comparatively same condition and a series of specific hybridization maps corresponding to each kinds of bacteria are obtained.Conclusions Salmonella spp., Shigella spp., Staphylococcus spp., Proteus spp., Escherichia coli, Yersinia enterocolitica, Listeria monocytogenes and Vibrio parahaemolyticus are detected and identified by the technology of gene chip. The accuracy, range, and discriminatory power of the assay can be continually improved by adding further oligonueleotides to the arrays without significantly increasing complexity or cost.

Objective To test and compare CA19-9,CEA with Specific N-Glycans in early-middle stage pancreatic carcinoma serum to find a better tumor marker in early pancreatic carcinoma .Methods To find the difference of N-Glycans structure ,DSA-FACE was performed in 35 early middle stage pancreatic carcinoma patients and 50 healthy human .Meanwhile ,we tested CA19-9 and CEA in the pancreatic carcinoma patients at the same stage.Result The serum N -glycan profiles of pancreatic carcinoma was identified by the DSA -FACE technique.The results showed that between pancreatic carcinoma patients and healthy persons ,there were significant differences in N -glycans.The peak 13,14 and 17 were the most significant peaks which would be most likely picked as a new tumor marker of pancreatic carcinoma .Taking log(p14 ×p17/p13)as indicator of the ROC curve analysis,and the area under ROC curve was 0.799 ±0.050 with 84.9% sensitivity and 68% speci-ficity.Meanwhile,the sensitivity of CA19-9 was 61.2%,the sensitivity of CEA was 11.7%.Conclusion The Peak 13,14 and 17 are the most significant peaks which would be picked as a new tumor marker of pancreatic carcinoma.Espicially,its sensitivity is superior to CA19-9、CEA for early middle stage patients .

Objective To investigate the structural changes in specific serum N-glycans in pancreatic cancer patients and to identify the specific serum maker of pancreatic cancer.Methods The pancreatic cancer patients who visited the Third Affiliated Hospital of Harbin Medical University from June 201 1 to December 2013 were assigned to preoperative serum group (123 cases)and postoperative serum group (78 cases);healthy controls whose serum samples were collected in the Physical Examination Center were selected as control serum group (271 cases).DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis (DSA-FACE)was used to analyze serum N -glycans and compare them between the three groups.Results The serum N-glycan profiles in pancreatic cancer patients were identified by DSA-FACE.The results indicated that N-glycan peak 8 in preoperative serum group was significantly lower than those in control serum group (t=2.735,P<0.05)and postoperative serum group (P<0.05),but no significant difference was found between the postoperative serum group and control serum group.Conclusion N-glycan peak 8 can be considered as a serum marker of pancreatic cancer.

Objective To evaluate the effect on postoperative urinary and sexual function of the radical operation with autonomic nerve preserving for mid low rectal cancer.[WT5”HZ]Method [WT5”BZ]Radical operation with autonomic nerve preserving was performed on 188 cases of rectal cancer. Nerves were fully preserved in 98 cases of Dukes A stage,one or both side nerves preserved in 78 cases of stage B, only S 3,4 nerve preserved in 12 cases of stage C.[WT5”HZ]Result [WT5”BZ]The postoperative voluntary micturition developed at 8 5 days for Dukes A; 9 0 days for stage B; and 16 8 days for stage C. Erection and ejaculation remained in Dukes A, in 96% and 100%;stage B, in 47 4% and 50%;stage C, in 0 and 0. The local recurrence after the operation accounted for 12 8%(24/188), among them Dukes A,B,C were 4 1%,21 9%, and 33 3% respectively.The 5 year survival rate were 77 6%,52 6% and 33 3% respectively.[WT5”HZ]Conclusion [WT5”BZ]The radical operation with autonomic nerve preserving for rectal cancer effects a better life quality not at the expense of local recurrence and reduction of 5 year survival rate.

Thyptase,a predominant serine protease in mast cells,is released by mast cell degranulation in an enzymatically active form.Recent research suggests that ttyptase plays important roles in mast cell mediated anaphylaxis,angiogenesis,initiation and promotion of neoplasm.This paper reviews tryptase's struture,molecular biology,biochemistry,processing,activation and its relationship with diseases.