ve To analyze and forecast the air pollution by SO2 and NOx in area of Shanghai railway station. Methods Based on the data on concentrations of SO2 and NOx in the air of the monitoring locations in area of Shanghai railway station during 1988~1999, using grey system mode, the model of grey dynamic forecast was es-tablished and was applied for forecasting the concentrations of SO2 and NOx in air during 2000~2002. Results During 1988?1999, the concentrations of SOz and NO, in air of area of Shanghai railway station had declined grad-ually. The forecasted average concentration of SO2 was 0.021 6 mg/m3 in the 1st and 4th quarters, 0.014 2 mg/m3 in the 2nd and 3rd quarters respectively. The forecasted average concentration of NOx was 0.070 6 mg/m3 in the 1st and 4th quarters, 0.049 2 mg/ m3 in the 2nd and 3rd quarters during 2000~2002 respectively. Conclusion This grey model was suitable for forecasting air pollution by SO2 and NOx in area of Shanghai railway station.

AIM: To study the apoptotic effect of TNFα on rat pulmonary microvascular endothelial cells (PMVEC) and the influences of Fas, NF-κB in its mechanism. METHODS: Apoptosis of PMVEC was analyzed and quantitated with TUNEL, flow cytometer. The distribution of NF-κB was detected via histoimmunochemical staining in TNF-treated cells and the control. Northern blot was applied to assess the influence of TNF on PMVEC Fas expression. Fas antibody was used to investigate the apoptotic effect of Fas on PMVEC. Activation of caspase-8 was detected with Western blot. Expression of caspase-3 was analyzed with histoimmunochemical staining. RESULTS: After treatment with 5×108 U/L TNF for 24 hours, viable PMVEC significantly diminished. Apoptosis rate was 14.0%±3.1% detected with TUNEL, and 13.1% with flow cytometer. Histoimmunochemical staining showed that NF-κB relocated from cytoplasm to the nuclear. When the cells were co-cultured with TNF and APDC, an NF-κB inhibitor, less cells were viable and more cells were positively stained with TUNEL. Fas expression in PMVEC was elevated treated with TNF. Apoptosis in PMVEC was found aggravated, when the cells were co-cultured with TNF and anti-Fas antibody. The positive rate was 24.1%±1.5% with TUNEL. Increase of caspase-8 activation was manifested by Western blot following TNF stimulation. Caspase-3 expression was found elevated using histoimmunochemical staining. Cell permeable caspase-3 inhibitor significantly ameliorated PMVEC apoptosis induced by TNF. CONCLUSION: 1. Large dose of TNF(5×108 U/L) can induce apoptosis in rat PMVEC. 2. NF-κB has a protective effect on PMVEC apoptosis. 3. TNF up-regulates Fas expression in PMVEC. And the latter takes a part in apoptosis. 4. TNF induced caspase-8 activation in PMVEC, and more caspase-3 was expressed. These may be involved in PMVEC apoptosis induced by TNF.

Acupuncture Therapy ; Adolescent ; Female ; Humans ; Lupus Erythematosus, Systemic ; complications ; Optic Nerve Diseases ; etiology ; therapy ; Retinal Degeneration ; etiology ; therapy

Objective To investigate the therapeutic effects of recombinant human interleukin-11 (rhIL-11) on the vaginal epithelium mitosis of estrone periodical mice and the exprssion of PCNA.Methods The vaginal epithelium mitosis of estrone periodical mice was used as the epidermis hyperplasia model, rhIL-11 action in regulating the epidermis hyperplasia was observed and the expression of PCNA was detected by immunohistochemistry.Results The rhIL-11 significantly inhibited the mitosis of mouse vaginal epithelium, decreased the expression of PCNA ( P <0.01).Conclusion The rhIL-11 has good efficacy in treating the epidermis hyperplasia of psoriasis by inhibiting the mitosis of epithelium and decreasing the expression of PCNA.

Objective To investigate the effect of Zhonghui Tangmaikang on clinical symptom, blood sugar and lipid of pre-diabetes patients. Method Seventy-two patients of pre-diabetes were divided into 2 groups randomly (36 patients of Zhonghui Tangmaikang group, 36 patients of metformin hydrochloride group), the course of treatment was 12 weeks. The changes of clinical symptoms, FPG, OGTT 2 h PG, TC, TG, LDL-C and HDL-C of the two groups were observed. Result After treatment, the blood sugar of two groups all descended prominently than before (P

Objective:To summarize the differences and similarities in the detection methods for residual solvents between Safety and Technical Standards for Cosmetics 2015 edition and Chinese Pharmacopoeia 2015 edition so as to provide reference for the improve-ment of the detection methods for residual solvents in Safety and Technical Standards for Cosmetics. Methods:The type and limitations of residual solvents and the characteristics of the test methods for residual solvents between Safety and Technical Standards for Cosmetics 2015 edition and Chinese Pharmacopoeia 2015 edition were compared and analyzed. Results: The detection methods for residual sol-vents in Chinese Pharmacopoeia were more detailed. The detection methods for residual solvents in Safety and Technical Standards for Cosmetics were general detection method, and the process could be applied in the detection of more solvents. Some detection methods were short of limitations. Conclusion:The control of residual solvents in cosmetics should be improved if the limitations table of the limiting used solvents is introduced into Safety and Technical Standards for Cosmetics referring to Chinese Pharmacopoeia and increase the types of residual solvents detected by the general methods.

AIM:To study the apoptotic effect of TNF? on rat pulmonary microvascular endothelial cells (PMVEC)and the role of Fas, NF-?B in its mechanism. METHODS: Apoptosis of PMVEC was analyzed and quantitated with TUNEL, flow cytometer. Northern blot was applied to assess the influence of TNF? on PMVEC Fas expression. Fas antibody was used to investigate the apoptotic effect of Fas on PMVEC. Activation of caspase-8 was examined by Western blot. Expression of caspase-3 was analyzed with histo-immunochemical staining. RESULTS:Growth curve showed that TNF? suppressed endothelial cell proliferation in a dose-dependent manner. After treatment with 5?10 6 U/L TNF?, apoptotic rate was 14.0%?3.1% detected with TUNEL, and 13.1% with flow cytometer. When the cells were co-cultured with TNF? and APDC, an NF-?B inhibitor, less cells were viable and more cells were positively stained with TUNEL. Fas expression in PMVEC was elevated after TNF? treatment. Co-culturing with Anti-Fas antibody aggravated PMVEC apoptosis. Caspase-8 activity and caspase-3 expression was elevated. Caspase-3 inhibitor significantly ameliorated PMVEC apoptosis. CONCLUSION: Large dose of TNF? (5?10 6 U/L) can induce apoptosis in rat PMVEC. NF-?B has an anti-apoptotic effect in PMVEC. TNF? up-regulates Fas expression in PMVEC, and the latter takes a part in apoptosis. Caspase-8 and caspase-3 are involved in PMVEC apoptosis induced by TNF?.

Objective To study the efficacy of JueDuYin in the treatment of benign prostatic hyperplasia (BPH). Methods 120 cases were randomly recruited into a treatment group and a control group, with 60 cases in each group.The treatment group was given JueDuYin, one dosage per day, while the control group was administrated with Finasteride, 5mg per day. After three courses of twelve weeks' treatment, the indications of international prostate symptom score (IPSS), residue urine (RU), Qmax and total prostatic value (IPV) were observed. Results There was significant changes in the valve of IPSS,RU, Qmax for both groups after the treatment compared (P<0.05～0.01). The therapeutic effects of the treatment group was obvious better than the control group (P<0.05).

Acupuncture Therapy ; Adult ; Aged ; Aged, 80 and over ; Combined Modality Therapy ; Exercise Therapy ; Female ; Humans ; Male ; Middle Aged ; Osteoarthritis, Knee ; therapy ; Treatment Outcome

Adipocytes ; chemistry ; Blood Vessels ; Cytokines