Objective:To study its effect on the expression of BDNF from injured astrocytes after HSP70 were blocked by HSP70-antibody. Methods:We performed scratch injuries in the cultured rat astrocytes,and added HSP70-antibody into the nu- trient medium at once after injury(intervention group).Then,at 30min,24h,4d,7d after injury,the expression of brain-derived neurotropic facto(rBDNF)was studied by immunocytochemical and reverse transcriptase-polymerase chain reaction(RT-PCR).The immunocytochemical pictures were analyzed by image analysis system to abtain the average optical density of BDNF. Results: The expression of BDNF increased gradually with injury time.Compared with the control group,the average optical density of BDNF in the intervention group were significantly reduced(P

No abstract available.
Humans

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Bone Neoplasms ; pathology ; therapy ; Catheter Ablation ; Humans ; Lung Neoplasms ; secondary ; surgery ; Neoplasm Recurrence, Local ; Osteosarcoma ; pathology ; secondary ; therapy ; Salvage Therapy

BACKGROUND: Until now, the total correction of tetralogy of Fallot has remained controversial and the efficacy of balloon dilatation of the pulmonary valves in tetralogy of Fallot has not been confirmed. We tried balloon dilatation of the pulmonary valves in 9 patients with tetralogy of Fallot to evaluate its effect on oxygen saturation and pulmonary arterial growth. METHOD: In nine patients with tetralogy of Fallot was undergone balloon dilatation since 1992 until August in 1999. Before starting the procedure, we measured the diameters of the pulmonary annulus, both pulmonary arteries and descending aorta. Systemic oxygen saturation and shunt amount were also measured before and after the procedure and systemic oxygen saturation two months later. RESULTS: The mean value of the pulmonary annulus diameters was 5.36+/-1.12 mm and their z value was -4.0+/-1.4. The systemic oxygen saturation increased from a mean value of 64.0+/-19.6% to 82.4+/-8.4% right after the balloon dilatation and 82.0+/-7.9% two months later. McGoon ratio increased from a mean value of 1.66+/-0.33 before the procedure to 1.91+/-0.37 two months later. The mean value of shunt amount(Qp/Qs) was 0.52 before the procedure and 0.84 immediately after the procedure. Furthermore, there occurred no significant complication during and after the procedure. CONCLUSION: The balloon dilatation of the pulmonary valves in tetralogy of Fallot seems relatively safe and produces an immediate effect on cyanosis. And the procedure improves the growth of pulmonary arteries. However, further research and evaluation are needed.
Aorta, Thoracic ; Cyanosis ; Dilatation* ; Humans ; Oxygen ; Pulmonary Artery ; Pulmonary Valve* ; Tetralogy of Fallot

Objective To evaluate the value and limitation of CTA, MRI and DSA in the diagnosis of the cerebral venous malformation. Methods CT angiography in 8 cases and MRI in 10 cases were performed among 12 cases of cerebral venous malformation confirmed by carotid angiography . Results All cases had typical DSA appearance and it could be seen both the number and the direction of the guide vein. The typical “Medusa-head” sign could be seen on MRI and could be more obvious after contrast enhancement.The other complications might be seen on the postcontrast. On CTA,both the lesion itself and the “caput medusae”sign could be displayed.Conclusion In diagnosis of cerebral venous malformation,DSA is the gold standard,CTA is the significant method in diagnosing and following up,while MRI is the most useful method.

Recent developments on separation and analysis of bacterial cells by capillary electrophoresis are reviewed. Based on bacterial surface characterization, capillary electrophoresis technique can be utilized for rapidly identify and quantify intact bacteria and microbes at a certain phase. The microbe-molecule interactions can also be described. The separation of microbes that can not be cultured in lab appears to be possible by this method. It makes profound effects on separation science and microbiology to establish capillary electrophoresis procedure for the separation and analysis of bacterial cells.

The paper introduces relevant concepts of parallelization,including Mapreduce programming frame and distributed document system of Hadoop,optimizes the traditional K-means algorithm,establishes parallel K-means clustering model,explores the medication rules of Traditional Chinese Medicine (TCM) for hypertension treatment,digs into 8 groups of prescriptions for differentiation treatment of hypertension through TCM,verifies authenticity of the conclusion by combining the theories of TCM.

OBJECTIVE:To observe therapeutic efficacy of Xinyi biyan pills combined with budilaide in the treatment of aller-gic rhinitis and its effects on related inflammatory factors. METHODS:A total od 120 patients with allergic rhinitis were randomly divided into observation group and control group,with 60 cases in each group. Control group was treated with Budesonide nasal spray,spraying once each nostril(64 μg/spray),2 times a day. Observation group was additionally treated with Xinyi biyan pills 3 g,3 times a day. Treatment course lasted for 4 weeks. Clinical efficacies of 2 groups were observed and compared. The changes of total score of clinical symptoms and signs were observed before and after treatment. The contents of IgE and LTE4 were deter-mined,and the occurrence of ADR was recorded. RESULTS:Three patients of observation group and 4 patients of control group fell of the study. Total response rate of observation group was significantly higher than that of control group,with statistical signifi-cance (84.21% vs. 73.21%,P<0.05). Before treatment,there was no statistical significance in total score of clinical symptoms and signs,IgE and LTE4 levels between 2 groups (P>0.05). After 2 and 4 weeks of treatment,total score of clinical symptoms and signs,IgE and LTE4 levels in 2 groups were significantly lower than before treatment,and the observation group was signifi-cantly lower than control group,with statistical significance (P<0.05). No obvious ADR was found in 2 groups. CONCLU-SIONS:Xinyi biyan pills combined with budesonide show good therapeutic efficacy for allergic rhinitis,improve clinical symp-toms and signs significantly and reduce inflammatory factors levels with good safety.

I have examined the effects of a growth factor, transforming growth factor(TGF)-B 1, on the rates of proteoglycan synthesis, aggregation potenital, and phenotypic expression of proteoglycans from human cervical intervertebral discs maintained in a cell culture system. A cell culture system for transitional and nuclear regions of degenerated human cervical intervertebral disc disc was devised to assess the biosynthetic response, assayed by 35S-sulfate incorporation into proteoglycan and protein synthesis assayed by 35S-methionine incorporation. And I had the data as the TGF-B 1 has an effect on the proteoglycan synthesis in quantitative and qualitative analysis in the cell culture system of the human intervertebral disc. TGF-B 1 may be used as a therapeutic alternative to degenerated disc disease.
Cell Culture Techniques ; Humans ; Intervertebral Disc ; Proteoglycans ; Transforming Growth Factors

OBJECTIVE: To review the pharmacological actions and related clinical research about the efficacy and safety of lomitapide, which is a new kind of medication for cholesterol disorder-microsomal triglyceride transfer protein inhibitor. METHODS: The domestic and oversea pertinent literatures since 2007 were searched. RESULTS: Lomitapide can performe well in clinic effects and tolerance for homozygous familial hypercholesterolemia. CONCLUSION: Lomitapide can be an effective adjuvant for patients with homozygous familial hypercholesterolemia.