Ribonucleotide reductase ( RR ) is a rate-limiting enzyme, and it is responsible for reducing ribonucleotides to their corresponding deoxyribonucleotides , which are the building blocks required for DNA replication and repair .Recent studies have revealed that RR activity is associated with DNA replication in virus , and RR inhibitors have been used for clinical antiviral treatment .This paper reviews research progress on RR and its inhibitors , including the classification , structure and function of RR; the classification, mechanism and clinical application of RR inhibitors in antiviral therapy and the future prospects of RR inhibitors .

Objective To study the changes of serum protein spectrum in patients with systematic lupus erythematosus (SLE) in order to screen specific protein markers. Methods Serum samples from 72 patients with SLE and 85 age- and sex-matched controls were assessed using surface-enhanced laser desorp-tion/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) with weak cation exchange (CM10) pro-rein chip. Forty samples from the patients and 50 control samples were randomly selected to serve as a pre-liminary training set; significantly different protein peaks were automatically chosen for the system training and development of a decision classification tree model. The validity of the model was then challenged with a blind test set (including another 32 samples from patients and 35 from human controls). Results A total of 73 effective protein peaks were detected within the mass/charge ratio (m/z) interval 2000 - 50000, among which, 15 protein peaks significantly differed between patients with SLE and controls (P < 0.01). Three pro-tein peaks with an m/z value of 4001, 6305 and 7356 were automatically chosen as a biomarker pattern in the training set that discriminated patients with SLE from controls with a sensitivity of 90.0% (36/40), speci-ficity of 92.0% (46/50) and accuracy rate of 91.1% (82/90). When the SELDI marker pattern was tested with the blinded test set, it yielded a sensitivity of 87.5% (28/32), specificity of 91.4% (32/35) and accuracy rate of 89.6% (60/67). Conclusions SELDI-TOF-MS protein chip could be used to screen serum protein for SLE, and the decision classification tree model with these biomarkers may favor the diagnosis of SLE.

Objective To evaluate the cellular toxic and release of pharmaceutical dressing.Method Following the State standard GB/T14233.2-2005.the L929 cellular merphology was observed by inveded microscopy after 72h and proliferation of the cells was examined using mitochondrial function(MTT)assay.Relative growth rate (RGR)was calculated and cytotoxicity grade was evaluated by absorbency(OD)data.With PBS7.4 as dissolution media,and(32±0.5)℃as dissolution temperature,the release rate was determined with UV method with the determination wavelength of 288 nm and the dissolved liquid in 1/6,1/2,1,3,16,24,36 and 48 h.Results The average cell RGR of the pharmaceutical dressing is 91.25%and reaches 1 cytotoxicity grade.L929 cellular morphology is normal.Pharmaceutical dressing release accord to Higuchi equation,and the simulated equation is M_t/M_∞=0.3271t~(0.239).Conclusions Biologic compatibility of the pharmaceutical dressing is good,and the release of levofloxacin from the pharmaceutical dressing is sustained in vitro.

In recent years, researchers have become more interested in fish scrap collagen collection .Fish collagen, thanks to its low antigenic and hypoallergenic properties , is superior to that of higher animals .Meanwhile , fish collagen derives from a wealth of sources and has a moderate price .Based on recent fish collagen extraction and modification , this article summarizeds extraction technologies , such as hot-water, chemistry, bio-enzyme, compound extraction and such modification methods as physical and chemical processes to improve the thermal stability .

The effect of the basic fibroblast growth factor to bone regeneration and its mechanical are introduced, as well as the relevant micro sphere controlled release and scaffold materials. The current restrictions on clinical application are discussed. The status of the sustained-release technology is focused. There are broad prospects for the application of basic fibroblast growth factor sustained release on bone regeneration.

Nano-hydroxyapatite has been widely used as reconstructive and prosthetic material for osseous tissue,owing to its excellent biocompatibility and tissue bioactivity.But the poor mechanical property of hydroxyapatite restricts its further application.In order to enhance the comprehensive performance of the material,many researches have been dedicated to the synthesization of the composite materials.This article reviews the main preparation methods of nano-hydroxyapatite and the advancement in research of its composite.The directions in this research area are described as well.

Shape-memory polymers(SMPs) can retain a temporary shape after pre-deformation at an elevated temperature and subsequent cooling to a lower temperature.When reheated,the original shape can be recovered.The development of the research on polyolefin,polyurethanes,polyester and some other shape-memory polymers were introduced and their applications in medical equipment were reviewed.

AIM:To investigate the correlation between matrix metalloproteinase-9(MMP-9),tissue metalloproteinase inhibitor-1(TIMP-1),MMP-9/TIMP-1 and carotid atheromatous plaque stability in cerebral infarction patients.METHODS:80 patients with cerebral infarction were categorized as microemboli-negative group(n=70)and microemboli-positive group(n=10),20 normal human were served as control group.The MMP-9 and TIMP-1 levels in plasma were determined by mean of ELISA in 3 groups.RESULTS:The levels of MMP-9 and TIMP-1 in plasma were significantly higher in cerebral infarction patients than those in control group(P

Purpose To prepare bFGF-PLGA microspheres and to investigate the characteristics. Methods The bFGF-PLGA microspheres were prepared by W_1/O/W_2 multiple emulsion volatilizing method, the morphology was investigated using scanning electron microscope (SEM), the ELISA method was used to establish the regression equation and to detect the drug loading amount and encapsulation efficiency, as well as sustained-release profile in vitro . Results The microspheres seemed to be smooth and uniform with mean particle size of (0.75 ±0.08) μm,the the drug loading amount and encapsulation efficiency were [(59.9± 1.9) × 10~(-3)] % and (79.9±2.8)%, respectively, the accumulative release ratio was up to 80 % in the continuous period of forty-five days. Conclusion The bFGF-PLGA microspheres have better pharmaceutical properties and long-time sustained release effect in vitro.