In tendering-based purchase of medical equipment, the specifications and parameters in tendering documents are the key factors. This paper presents the way to compile related document on the basis of the experience.

OBJECTIVE To discuss the gentamycin-induced ototoxity and mechanism on cochlear outer hair cells. METHODS All the guinea pigs were randomly divided into 3 groups. Gentamycin, gentimycin plus furosemide and normal saline were intraperitoneally injected for 6 days respectively. The amplititude of distortion product otoacoustic emissions (DPOAE)urface prepration of cochlear basilar membrane, hair cells’ apoptosis and expression of caspase-3 after injection were analyzed. RESULTS Reduction of the DPOAE amplitude, hair cell damage, apoptosis and Caspase-3 expression were observed in group injected with gentamycin as well as in group injected with gentamycin plus furosemide. CONCLUSION Gentamycin should induce hair cell damage in the cochlea of guinea pigs and Caspase-3 plays an important role in the process of hair cell apoptosis.

OBJECTIVE To investigate the location and degree of pharyngeal narrowing in patients with ob-structive sleep apnea-hypopnea syndrome(OSAHS). METHODS Fifty patients with OSAHS were divided into two groups. The first group was examined with single layer helix CT at an awaken state. The second group was examined with single and 16 layer helix CT at an asleep state. Control group was 225 healthy adults. RESULTS The average minimum diameter of pharynx at soft palate level was 5.85 mm and the aver-age minimum section proportion was 50.3 mm2 in the first group. The average minimum diameter and the av-erage minimum section proportion of pharynx at soft palate level were 2.88 mm and 31.5 mm2, and 1.62 mm and 6.3 mm2 as examined with single layer and 16 lay-ers helix CT scan respectively in the second group. CONCLUSION CT can be used as a method for esti-mating the degree of pharyngeal narrowing in patients with OSAHS. 16 layer helix CT scan can reflect the actual pharyngeal narrowing in OSAHS patients while sleeping more accurately.

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With wide range applications of the ultra-large-scale integrated circuits, multilayer circuit board and components surface welding technology, clinical medical engineers have been confronted with challenges in the service and maintenance of largescale medical equipments and the traditional model has faced the great challenges too. The clinical medical engineers should be actively get involved in operation, maintenance and service of large-scale medical equipment, in the same time; it's necessary for buying suitable service supply, protecting the smooth function of large- scale equipment and meets the clinical needs.

As the development of clinical medicine has depended on modern medical equipment increasingly,clinical medical engineers have become the bridges between modern clinical medicine and engineering.They keep the normal running of hospitals and promote the hospital modernization.The equipment service & maintenance isn't the only responsibility of medical engineers.In fact,engineers should be responsible for all medical activities relevant to engineering equipment.The main tasks of engineers should shift from the equipment service & maintenance to the application safety and quality control in the whole process of the equipment usage,which include equipment application safety and risk control,failure service and preventive maintenance,quality control and management,measurement and monitor of adverse event,etc.

OBJECTIVE To investigate the expression of transcription factors Snail,Slug and cell adhesion molecule E-cadherin in nasopharyngeal carcinoma(NPC)and evaluate their relationship with clinical pathological features and metastases.METHODS Immunohistochemistry and quantitative polymerase chain reaction were carried out to detect the expression of Snail,Slug and E-cadherin in 65 NPC samples and normal nasopharyngeal mucosa(18 cases).The expression of Snail and Ecadherin in distinct differentiation nasopharyngeal carcinoma was analyzed and the relationship of them with clinical pathological features was discussed.RESULTS Real-time PCR analysis and immunohistochemistry showed that Slug was elevated with increasing tumor grade.E-cadherin expression was reduced corresponding with prognostic indices and tumor.Snail showed no obvious change.The relative expression levels of E-cadherin mRNA were significantly different between lymph node metastasis group and nometastasis group(P

Objective To establish a novel method by integrating immunomagnetic bead enrich-ment with immunochromatography for the detection of influenza A virus. Methods The immunomagnetic beads were prepared by using EDC/NHS method and then coupled with monoclonal antibodies against influ-enza A virus. A direct immunomagnetic beads-based immunochromatography for the detection of influenza A virus was developed by using double-antibody sandwich method and immunochromatography, which was fur-ther combined with immunomagnetic separation to establish the novel integrated method of immunomagnectic bead enrichment and immunochromatography. Clinical throat swab samples collected from patients with influ-enza A virus infection and healthy subjects were analyzed by the novel method and the results were compared with those by using the conventional colloidal gold immunochromatography to evaluate the specificity, sensi-tivity and positive coincidence rate of this established method. Results The direct immunomagnetic beads-based immunochromatography and the colloidal gold immunochromatography showed no significant differences in specificity and sensitivity and could be used to identify influenza A virus-positive samples with cycle threshold ( Ct) values less than or equal to 22 obtained by real-time PCR assay. The integrated method could identify positive samples with Ct values less than or equal to 28, indicating that the novel method was more sensitive. Conclusion The novel method by integrating immunomagnetic bead enrichment with immunochroma-tography was successfully established and suitable for the rapid and on-site detection of influenza A virus.

Utilizing micro-pump and under the control of SCM,the tourniquet can automatically control antimemorrhagic pressure,antimemorrhagic time and loosing time.It is suitable for the automatic hemostasis of limbs.There are two working modes for medical service staff to select including air automatic inflation & releasing mode and manual air releasing mode.A new design of the sleeve bandage enable the wounded arms and legs use the same tourniquet.It is easy and convenient to release and repressurizing,which is suitable for the wounded to self operated.

Using the new negative pressure biopsy technology, the automatic negative pressure gun and the specific puncture needle for the biopsy have been developed. Renal biopsies were conducted in 1136 cases, with the success rate being 99.9%, and 29 cases gross hematuria while the hepatic biopsies were conducted in 16 cases, mass biopsies in 3 cases with the success rates being 100% respectively. The biopsy gun has the advantages of easy manipulation, higher success rate and lower incidence of complications.
Adolescent ; Adult ; Aged ; Biopsy, Needle ; instrumentation ; methods ; Child ; Child, Preschool ; Equipment Design ; Humans ; Kidney ; diagnostic imaging ; pathology ; Liver ; diagnostic imaging ; pathology ; Middle Aged ; Needles ; Ultrasonography