AIM To develop an HPLC method for the simultaneous content determination of honokiol,magnolol,dehydrotumulosic acid,tumulosic acid,polyporenic acid C,3-epidehydrotumulosic acid,dehydropachymic acid and pachymic acid in Jinsang Liyan Capsules (Magnoliae officinalis Cortex,Poria,Aurantii Fructus Immaturus,etc.).METHODS The analysis of methanol extract of this drug was carried out on a 35 ℃ thermostatic Kromasil C18 column (4.6 mm × 250 mm,5.0 μm),with the mobile phase comprising of acetonitrile-0.05％ phosphoric acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 294,241 and 210 nm.RESULTS Eight constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were more than 96.0％ with the RSDs of less than 2.0％.CONCLUSION This simple and reproducible method can be used for the quality control of Jinsang Liyan Capsules.

In this study we have examined the state of actin polymerization in B cells from different development stages in bursa of Fabricius of Peking duck by indirect immunofluorescence technique using antibody against actin. In B cells from bursa of Fabricius of 26th day embryo and 3th week after hatching, the polymeric actin was the main form in the actin pool. In B cells from bursa of Fabricius of 12th week after hatching, however, the monomeric actin was the main form, which might be a result of the shift from the polymeric actin pool to the monomeric pool. We concluded that the state of actin polymerization might be an important factor in the cellular functions of B cells of bursa of Fabricius.

BACKGROUND:Bone marrow mesenchymal stem cell (BMSCs) transplantation has been reported to promote the repair of uterine injury;Shenghuazhixue decoction functions to promote blood circulation and remove blood stasis, and dysmenorrhea. OBJECTIVE:To investigate the effect of BMSCs transplantation combined withShenghuazhixue decoction on subinvolution of uterus effect and the underlying mechanism. METHODS:The model of subinvolution of uterus was established in 40 femaleSprague-Dawley rats, followed by randomly divided into four groups: control,Shenghuazhixue decoction, BMSCs, and combination groups. The control group received no treatment; at the 1st day after modeling, 1×107/L BMSCs (1 mL) were injected into the rat myometrium in the BMSCs and combination groups before laparotomy; the rats in theShenghuazhixue decoction group received no treatment after incision of uterus, and then rinsed using normal saline before closing the abdomen; theShenghuazhixue decoction and combination groups were given 8 mL/(kg?d)Shenghuazhixue detectionvia gavage at the 1st day after modeling, once daily, for consecutive 7 days. Afterwards, the relevant indexes were detected. RESULTS AND CONCLUSION:Compared with the control group, the body weight, uterine wet weight and uterine index in the combined group were significantly decreased after treatment (P < 0.05). The body weight and uterine wet weight showed significant differences betweenShenghuazhixue decoction and control groups (P < 0.05). Compared with the control group, the expression levels of intrerleukin-1 and tumor necrosis factor-α were significantly decreased, and the serum level of thromboxane B2 was significantly increased in theShenghuazhixue decoction group after treatment (P < 0.05). After treatment, the expression levels of intrerleukin-1 and tumor necrosis factor-α were significantly decreased, and the level of vascular endothelial growth factor was significantly increased in the BMSCs group compared with the control group (P < 0.05). Compared with the control group, there was significant decease in the expression levels of intrerleukin-1 and tumor necrosis factor-α, and significant increase in serum level of thromboxane B2 and vascular endothelial growth factor in the combination group (P < 0.01). These results suggest that BMSCs transplantation combined withShenghuazhixue detection can reduce postpartum body weight, uterine wet weight and index of uterus, and promote uterine involution in female rats, which may be through decreasing the levels of intrerleukin-1 and tumor necrosis factor-α as well as increasing the levels of thromboxane B2 and vascular endothelial growth factor.

Objective To discuss the efficacy of omeprazole and pantoprazole in treatment of peptic ulcer hemorrhage.Methods 80 patients with peptic ulcer hemorrhage were selected,they were divided into two groups randomly.The observation group (41 cases) was given pantoprazole by intravenous drip.The control group (39 cases) was given omeprazole by intravenous drip.The efficacy and safety of omeprazole and pantoprazole in treatment of peptic ulcer hemorrhage was evaluated by efficacy,pH before and after treatment,bleeding time,hospitalization and bleeding volume,and adverse reaction during treatment.Results The effective rate was 92.7％ in the observation group and 89.7％ in the control group.There was no statistical significance on effective rate between two groups.But the excellent rate of observation group was higher than that of the control group (P ＜ 0.05).Before treatment,the gastric acid was acidic.There were no statistical significance on pH value between two groups.After treatment,the pH value was increased in two groups.The pH value of observation group was higher than that of the control group (P ＜ 0.05).The hospitalization,hemostasis time and bleeding volume was shorter than that of the control group (P ＜ 0.05).During treatment,the patients given pantoprazole had less adverse reaction (P ＜ 0.05).Conclusion Pantoprazole and omeprazole are suitable for treating peptic ulcer hemorrhage.But the antacid and hemostatic effect of pantoprazole was better with high safety.It was worthy of clinical application.

Stenting is one of the effective approaches in the treatment of cerebrovascular stenosis. It may reduce the risk of ischemic stroke in patients with symptomatic and asymptomatic cerebrovascular stenosis. With the increasing number of stenting procedures in the treatment of cerebrovascular stenosis, various complications have received attention. This article reviews some common complications and their management.

Objective To evaluate the application of fast-FLAIR T 2WI and enhanced fat-suppressed T 1WI in diagnosis of meningeal disorders.Methods Fast-FLAIR T 2WI and enhanced fat-suppressed T 1WI imaging for 38 patients with meningeal disorders were contrastingly studied.Results Meningeal disorders foci were found in all patients. 55 foci were displayed with fast-FLAIR T 2WI and 59 with enhanced fat-suppressed T 1WI.Conclusions The fast-FLAIR T 2WI and enhanced fat-suppressed T 1WI are all useful to improve the detection of meningeal disorders.

The antimicrobial peptide magainin II is expressed in the skin of the African clawed frog, Xenopus laevis, and exhibits a broad spectrum of antimicrobial activity as well as tumoricidal properties at low concentrations. In addition, magaininII plays a synergistic role during antimicrobial and tumoricidal processes with another antimicrobial peptide PGLa that is also expressed in Xenopus laevis. The optimized cDNA sequence of magainin II and magainin II-PGLa hybrid peptide according to E. coli or Pichia pastoris codon usage frequency were synthesized and sub-cloned into prokaryotic expression vector pGEX and Pichia pastoris secreted expression vector pPIC9k. The resulting recombinant plasmids were named as pGEX-magainin II and pPIC9k-magainin II-PGLa. The GST-magainin II fusion protein was highly expressed in E. coli. Furthermore, magainin II was successfully purified by digestion with PreScission Protease to cleave the GST tag. Additionally, our data obtained from the ELISA revealed that magainin II -PGLa hybrid peptide was successfully expressed in Pichia pastoris. These experiments establish a useful system for further studies of these antimicrobial peptides.
Animals ; Escherichia coli ; metabolism ; Genetic Vectors ; Magainins ; biosynthesis ; genetics ; Peptides ; genetics ; metabolism ; Pichia ; metabolism ; Plasmids ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; Xenopus Proteins ; biosynthesis ; genetics ; Xenopus laevis

Influenza A virus can create acute respiratory infection in humans and animals throughout the world, and it is still one of the major causes of morbidity and mortality in humans worldwide. Numerous studies have shown that influenza A virus infection induces rapidly host innate immune response. Influenza A virus triggers the activation of signaling pathways that are dependent on host pattern recognition receptors (PRRs) including toll like receptors (TLRs) and RIG-I like receptors (RLRs). Using a variety of regulatory mechanisms, these signaling pathways activate downstream transcript factors that control expression of various interferons and cytokines, such as type I and type III interferons. Thus, these interferons stimulate the transcript of relevant interferon-stimulated genes (ISGs) and expression of the antiviral proteins, which are critical components of host innate immunity. In this review, we will highlight the mechanisms by which influenza A virus infection induces the interferon-mediated host innate immunity.
Cytokines ; immunology ; DEAD Box Protein 58 ; DEAD-box RNA Helicases ; immunology ; Humans ; Immunity, Innate ; Influenza A virus ; Influenza, Human ; immunology ; Interferons ; immunology ; Receptors, Pattern Recognition ; immunology ; Signal Transduction ; Toll-Like Receptors ; immunology

Objective To establish a method for determination of polysaccharides in Weixuening Granule, which can be used for quality control of the preparation. Methods Polysaccharides was determined by improved differential phenol-sulfuric method with glucose as reference, and the color conditions were optimized at the wavelength of 487 nm, with the concentration of phenol and sulfuric acid, and reaction time as investigated factors. Results The best color conditions of polysaccharides were 0.04 g/mL phenol 1 mL, 87.5% sulfuric acid 5 mL, and reaction time of 15 min. Glucose had a good linear relationship with absorbanceΔA at the range of 15.3-306.0μg, r=0.999 7. The average recovery was 103.5%, and RSD was 2.9%. The average content of polysaccharides in Weixuening Granule was 27.04%and RSD was 1.1%. Conclusion The method is simple, accurate, stable and reliable, and can be used to determine the content of polysaccharides in Weixuening Granule.

Objective To evaluate the diagnostic efficiency of Q?SPECT, CTPA, Q?SPECT/CT, and Q?SPECT/CTPA for pulmonary embolism (PE) in rabbit models. Methods (1) The PE models were constructed by injecting Gelfoam into the femoral vein of New Zealand rabbits ( n=30) . Q?SPECT, CTPA, Q?SPECT/CT and Q?SPECT/CTPA fusion images were obtained by integrated SPECT/CT. (2) All images were interpreted by two experienced nuclear radiologists who were blind to pathologic findings. The locations and numbers of lung lobes with PE were recorded respectively. ( 3) Serial sectioning of the lungs was per?formed and pathologically determined. (4) Se, Sp and Ac of different methods were compared using McNemar test;PPV and NPV were compared usingχ2 test. Kappa test was used to analyze the consistency between two nuclear radiologists. Kappa values<0.40 were interpreted as poor consistency, 0.40 to 0.75 as moderate con?sistency, >0.75 as good consistency. Results (1) Histologically confirmed emboli were present in a total of 26 pulmonary lobes and absent in 79 lobes. (2)The Se, Sp, Ac, PPV, and NPV of 4 imaging methods were:53.8%(14/26), 93.7%(74/79), 83.8%(88/105), 14/19, 86.0%(74/86) for Q?SPECT;73.1%(19/26), 96.2%(76/79), 90.5%(95/105), 86.4%(19/22), 91.6%(76/83) for CTPA;76.9%(20/26), 93.7%(74/79), 89.5%(94/105), 80.0%(20/25), 92.5%(74/80)for Q?SPECT/CT;88.5%(23/26), 91.1%(72/79), 90.5%(95/105), 76.7%(23/30), 96.0%(72/75) for Q?SPECT/CTPA. (3) McNemar test showed Q?SPECT/CT and Q?SPECT/CTPA had higher diagnostic Se for the detection of PE than Q?SPECT (χ2=4.167, 7.111, both P<0.05) , but without any significant difference with CTPA in diagnostic efficiency (χ2=0-2.250, all P>0.05) . Q?SPECT/CT had higher diagnostic Ac than Q?SPECT (χ2=4.167, P<0.05) . There was no significant difference between Q?SPECT/CT and Q?SPECT/CTPA in diagnostic effi?ciency (χ2=0.001-1.333, all P>0.05). (4)Kappa values of 4 imaging methods for radiologist 1 and 2 were 0.902, 0.915, 0.973, and 0.884. Conclusions Q?SPECT/CT imaging provides good Se and Sp. The diag?nostic efficiency of Q?SPECT/CT is better than that of Q?SPECT and is corresponded roughly to the efficien?cy of CTPA, Q?SPECT/CTPA. The diagnosis of two radiologists on Q?SPECT/CT images has the best con?sistency.