Objective To explore the dynamic changes and the meaning of T lymphocyte subpopulation in peripheral blood and its clinical significance in patients with acute cerebral infarction.Methods The numbers of CD3 +,CD4 + and CD8 + T lymphocyte in peripheral blood were examined with flow cytometry in 20 patients with acute cerebral infarction(CI) at the 3rd,5th,7th and 14th day after the onset.Results The numbers of CD3 + and CD4 + T lymphocytes and the ratio of CD4 +/CD8 + in the patients with acute CI were lower than those in normal group at the 3rd and 5th day after the onset (P0.05).Conclusion The immunological function of the cells in the patients with acute CI decreased obviously at the 3th and 5th day, and then restored partly at the 7th day, at last restored completely at the 14th day after the onset. It suggests that the focus time for the prevention of infective complications is in 10 days after the acute CI.

Humans ; Joint Dislocations ; Lunate Bone ; Wrist Injuries

Buttocks ; pathology ; Humans ; Muscle Neoplasms ; diagnosis ; Muscle, Skeletal ; pathology ; Synovitis, Pigmented Villonodular ; diagnosis

We carried out the stress relaxation and creep experiments on the aorta ascendens of five fresh dead bodies of normal chinese men who had died from impetuous trauma on the head. The stress relaxation and creep data and curves of the aorta ascendens were obtained. Then regression analysis was performed to get the reduced stress relaxation and creep functions. Finally, we analyzed and discussed the results of the experiment.
Adult ; Aorta ; physiology ; Elasticity ; Humans ; In Vitro Techniques ; Male ; Regression Analysis ; Stress, Mechanical ; Viscosity

Objective To investigate the atypical appearances of small hepatocellular carcinoma(SHCC) with triple-phase spiral CT enhanced scan, and its correlation with the histopathology .Methods The atypical CT signs in triple-phase and histopathologic changes of SHCC confirmed pathologically in 30 cases (32 lesions) were analysed.Results 32 atypical lesions were found in 30 patients,of them,14 lesions were hypodense in hepatic arterial phase(HAP),portal venous phase(PVP) and delayed phase(DP).10 lesions enhanced markedly in the AP,while these lesions became isodense or slight hyperdense in the PVP and DP.8 lesions were enhanced as ring like or punctual shape in the AP,and constant enhancement in PVP and DP.Conclusion The atypical appearances are present in triple- phase spiral CT scan in SHCC,the pattern of blood supply and the base of histopathology are usually the cause of these findings .

Objective: To explore the differences of the relative expression levels of plasma long chain non encoding RNA (IncRNA) MALAT1, LincRNA-p21 and GAS5 in the patients with multiple myeloma (MM) and chronic lymphocytic leukemia (CLL), and to clarify their significances in the differential diagnosis of MM and CLL. Methods: A total of 60 cases of MM patients (MM group), 60 cases of CLL patients (CLL roup) and 60 healthy persons after physical examinations (control group) were selected as the subjects. The plasma levels of IncRNA MALAT, LincRNA-p21 and GAS5 of the subjects in three groups were detected and compared. Results: The relative expression levels of IncRNA MALAT1 and GAS5 in plasma of the patients in MM group were significantly higher than those in the other two groups (P<0.05); the relative expression level of LincRNA-p21 in plasma of the patients in CLL group was significantly lower than those in the other two groups (P<0.05). The area under receiver operating characteristic curve (ROC) (AUC) of plasma LincRNA-p21 in the diagnosis of CLL was 0.850, its 95% confidence interval (CI) was 0.780-0.921. The AUC of plasma IncRNA MALAT1 and GAS5 in the diagnosis of MM were 0.898 and 0.815; their 95% CI were 0.836-0.959 and 0.740-0.890, respectively. The AUC of plasma IncRNA MALAT1, LincRNA-p21 and GAS5 in the differential diagnosis of CLL and MM were 0.878, 0.778 and 0.805, and their 95% CI were 0.814 - 0.942, 0.691 - 0.865 and 0.727 - 0.882, respectively. Conclusion: The incidence of MM is related with the high expressions of IncRNA MALAT1 and GAS5 in plasma and the incidence of CLL is related with the low expression of LincRNA-p21 in plasma. The relative expression levels of the above IncRNA can be used as the auxiliary indexes in the diagnosis of MM and CLL.

Objective: To study the effects of different pretreatment methods and culture temperatures on Seed Germination and seedling growth of Bupleurum chinense, in order to provide scientific basis for artificial cultivation of B. chinense. Methods: Several physiological indexes such as the weight, pure rate, content of moisture, the rate of water absorption per thousand seeds were measured. Adopting a double-layer filter paper culture method, the pretreated seeds were cultured in the incubator at 20 ℃ in the 40% light. In different culture temperature treatment groups, the seeds were soaked in distilled water 24 h and then cultured in the corresponding temperature incubator. The germination energy, germination percentage, germination index, vigor index of seeds, and the root length and the shoot height of the seedling were recorded. Results: The pure rate of seeds was 91.51%, the weight of one thousand seeds was (2.83 ± 0.03) g and the weight of seed after absorbing water was about 2.24 times more than the weight of naturally dried seed. The content of moisture of seeds was 7.3%. Different hormones had different effects on seeds, among them the effect of 0.6 mg/L 6-BA treatment was the best. Water bath could increase the seedling vigor index, and 40 ℃ of its temperature significantly promoted the seed germination and seedling growth. The germination rate of seeds was the highest under the condition of 15 ℃, but the root length and seedling height were lower. Conclusion: The suitable condition for seed pretreatment and culture temperature of B. chinense established in this research can effectively improve the germination rate and seedling rate, which is of great significance for seed breeding and artificial cultivation of B. chinense.

Bupleuri Chinensis Radix, as traditional Chinese medicine, has great use amount in the past dynasties, and the amount of the existing wild resources is not enough to provide market demand. In order to protect the wild resources and expand the source of medicinal materials, the research on the chemical composition, pharmacological action, clinical application and other aspects of Bupleuri Chinensis Radix and other plants in the same genus have been widely carried out. The paper reviews the study of medicinal plant resources of Bupleurum L. in China from the distribution and species, chemical composition of medicinal parts, resources protection and utilization of Bupleurum L. medicinal plants, etc, to provide new clues for Bupleurum L. resources development.

Proanthocyanidin (PA), as a kind of natural plant polyphenol, have a variety of biological functions, such as promoting remineralization, inducing collagen cross-linking, inhibiting protease activity and inhibiting bacteria. Therefore, PA could be broadly used in the clinical application of treatment and repair of deep caries in the future; for example, PA could promote dentin remineralization, improve resin-dentin bonding durability and improve the dentin acid erosion effect. This application potential of PA arises from several features, firstly, PA can not only promote dentin remineralization on its own or with other remineralizers but also exhibits antibacterial effects, which can inhibit acid production while reducing the formation of cariogenic pathogens and their biofilms. Based on the above features, PA can reduce the incidence of caries disease; thus, PA improves deep caries and long-term effects after treatment. In addition, PA added to adhesives or etch agents can improve the etching and bonding effect of dentin by inducing collagen cross-linking and inhibiting protease activity, thus achieving the ultimate goal of improving the bonding performance of deep caries. This paper summarizes recent progress of research on PA for the treatment and repair of deep caries, including the promotion of dentin remineralization and antibacterial activity as well as the improvement in dentin bonding and acid etching effect, to provide a more comprehensive reference for treating and restoring deep caries in clinical practice.

Preschool age(3-6 years old)is a critical period for visual development, and it is crucial to detect and treat visual problems in preschool children as early as possible. Visual acuity charts are important tools for screening visual issues in children. In China, the commonly used charts are the standard logarithmic visual acuity chart and the pediatric optotype chart, while overseas, the Lea, HOTV, and ETDRS visual acuity charts are frequently employed. Numerous studies have reported the measurability, repeatability, and sensitivity of these three charts in diagnosing visual-related problems in children. However, the application of these three charts is relatively limited in China. This article provides a comprehensive review of the design principles, clinical applications, and characteristics of these three visual acuity charts, so as to better understand their applicability and limitations in preschool children, and provide reference for the selection and improvement of vision examination methods in the future.