1.Effects of Tributyltin Chloride Exposure during Pregnancy on Development and Sex Hormone Level of Male Offspring Rats
Chengen WANG ; Jiliang SI ; Miao ZHANG
Journal of Environment and Health 2007;0(07):-
0.05). Compared with the control group,an obvious retarded development was observed from PND21-70 and the viscera coefficient of testis were increased significantly in 2.5 or 5.0 mg/(kg?d) group (P
2.Effects of Exposure to Tributyltin Chloride during the Critical Period for Sex Differentiation on Spermatogenesis Function and Activities of Enzyme in Testis in Rats
Miao ZHANG ; Jiliang SI ; Chengen WANG
Journal of Environment and Health 2007;0(08):-
0.05).Compared with the control group,body weights gains from PND21-70 were decreased significantly in the 5 and 2.5 mg/kg group(P0.05).No abnormal structure of testis was observed in all exposure groups.Conclusion Exposure to TBTC during critical period for sex differentiation may decrease body gain and increase spermatogenesis and the activities of LDH and SDH.
3.Effect of Gestation and Lactation Exposure to Low-dose Tributyltin on Development of Mice Offspring
Chengen WANG ; Jiliang SI ; Miao ZHANG
Journal of Environment and Health 2007;0(11):-
Objective To explore the effects of gestation and lactation exposure to low dose tributyltin(TBT) on development of offspring of Kunming mice.Methods The healthy adult pregnant Kunming mice were randomly divided into 4 groups,6 in each group,they were given doses of TBT(100,10 and 1 ?g/kg) by gavage from days 6 of gestation(GD6)to the end of lactation.The number of pups and the number of male,female of each dams were determined,and the body weight of pups on PNDs 1,3,5,7,14,21 was weighed.The eye opening from PND12 and the testes descent from PND13 and the vagina opening from PND20 were examined.Results Compared with the control group,the gestational body weight gain of dams decreased in 100 ?g/kg group(P
4.DIFFERENTIAL EXPRESSION OF CATHEPSIN D IN GERMINAL CENTER OF FOLLICULAR LYMPHOMA AND FOLLICULAR HYPERPLASIA AND ITS SIGNIFICANCE
Jiliang FENG ; Gaosheng HUANG ; Juanhong WANG
Medical Journal of Chinese People's Liberation Army 2001;0(10):-
Objective To study the expression of aspartic proteinase cathepsin D in the germinal center of follicular lymphoma and follicular hyperplasia. Methods Immunohistochemical staining was performed to detect the expression of cathepsin D in paraffin sections of 10 cases of follicular lymphoma and 9 cases of follicular hyperplasia. Results Cathepsin D was weakly expressed in the plasma of dendritic cells and histiocytes in the germinal centers of 9 out of 10 cases of follicular hyperplasia. The number of positive cells were smaller in the center of follicles, but larger in the periphery. It was more strongly expressed in the plasma of dendritic cells and histiocytes located predominantly in the peripheral areas of germinal centers of follicular lymphoma in 10 patients. Some germinal centers were surrounded by T lymphocytes in 4 cases of follicular lymphoma, and cathepsin D positive cells in these germinal centers were more in number than that in the germinal centers without being surrounded by T cells in other 6 cases of follicular lymphoma, in which positive sign even absent in some germinal centers. Conclusion Cathepsin D is a good marker for the dendritic cells and histiocytes, and its differential expression in follicular lymphoma and follicular hyperplasia is helpful for differential diagnosis of the diseases. Based on the former reports of cathepsin D with its important roles in antigen processing, we suppose that lower expression of this protease is probably due to the decreased ability of immunological response to tumor in follicular lymphoma patients.
5.Inhibitory effect of Garlic Polysaccharide on adriamycin-induced cardiotoxicity in mice
Wei YU ; Jiliang WU ; Hui WANG
Chinese Pharmacological Bulletin 1987;0(01):-
Aim To study the inhibitory effects and its mechanis ms of Garlic Polysaccharide (GP) on adriamycin(ADR)-induced cardiotoxicity in mic e.Mehtod ADR was injected intraperitoneally to induce myocardiu m injury model in mice. The activities of several serum and heart tissue enzymes were measured. With scanning electron microscope, the cardiac ultrastructural c hanges were examined.Results ADR (3 mg?kg -1 ip, qod?7) induced severe myocardial damages with the increasing activities of creatine kin ase (CK), lactate dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT) a nd inducible nitric oxide synthase (iNOS) (P
6.The Relation Between Treatment Based on Differential Diagnosis of Syndromes and Trace Elements in the Hairs of Aplastic Anemia Patients
Jiliang WANG ; Shulian YANG ; Guangyin CHEN
Journal of Traditional Chinese Medicine 1992;0(09):-
12 trace elements from the hairs of 20 cases of aplastic anemia were examined and compared with that from hairs of normal people as control. Results showed that in Yin-deficient type patients, Li, Ca, Si, Cr markedly decreased; in Yangdeficient type patients, Zn, Mg, Ba, Si, Ca, Li markedly decreased, while all 12 trace elements decreased in patients of deficiencies of both Yin and Yang. New approaches were also offered for treatment of aplastic anemia on the basis of differential diagnosis.
7.Antibacterial Activity of Rifampin Against Helicobacter pylori in vitro
Jiliang WANG ; Yurong TANG ; Guanghong DONG ; Xiuli WANG ; Yanfang ZHANG
Chinese Journal of Nosocomiology 2009;0(20):-
0.05).The prevalence of strains with resistance to rifampin was lower than that of strains with resistance to clarithromycin(P
8.Protective effect of garlic polysaccharide on cardiac myocyte injury by adriamycin
Wei YU ; Jiliang WU ; Hui WANG ; Wenliang ZHA
Chinese Pharmacological Bulletin 2003;0(09):-
Aim To observe the protective effects and its mechanisms of Garlic Polysaccharide(GP) on toxic cardiac myocyte induced by adriamycin (ADR). Methods Primary culture neonatal SD rat cardiac myocyte and ADR injury model were established. The activities of several superior fluid and cells enzymes were measured. Using MTT assay and flow cytometry, the apoptotic cardiac myocyte was shown. Results ADR increased the superior fluid creatine kinase (CK), lactate dehydrogenase(LDH), glutamic oxaloacetic transaminase (GOT), and augmented myocardial malondialdehyde (MDA) content, while decreased the superoxide dismutase (SOD) activities (P
9.Protective effect of ischemic preconditioning on liver ischemic-reperfusion injury of liver transplant in rats
Xincai QU ; Jiliang WANG ; Tao QIN ; Qichang ZHENG
Chinese Journal of General Surgery 1993;0(03):-
Objective To investigate the protective effect and mechanism of warm ischemic preconditioning of on liver cold ischemic-reperfusion injury of donor liver in rats. Methods Male Sprague Dawley rats were used as donors and recipients of orthotopic liver transplantation, the cold ischemia period and anhepatic phase was 120 min and 16~20 min respectively. Rats were randomly divided into 3 groups: sham-operation group, liver transplantation group, and ischemic pre-conditioning(IP) group. IP was performed with 5-min ischemia followed by 5-min reperfusion. Results After operation, the serum levels of ALT and superoxide were significantly higher in the groups of liver transplantation and IP group than in the sham-operation group(P
10.The characteristic and treatment of high anterior column fracture of acetabulum with intact true pelvic brim
Gouqing TAN ; Dongsheng ZHOU ; Bomin WANG ; Jiliang HE ; Baisheng FU
Chinese Journal of Orthopaedics 2011;31(11):1239-1244
ObjectiveTo investigate the results of open reduction with internal fixation for high anterior column fracture of acetabulum with intact true pelvic brim.MethodsFrom January 2006 to January 2010,12 patients suffered high anterior column fracture of acetabulum without involvement of true pelvic brim were identified.There were 9 males and 3 females,with the average of 35.6 years(range,29-46).The injury was caused by crush in 7 cases,smash of heavy object in 3 cases,and fall-down from height in 2 cases.These fractures were classified into three types:isolated high anterior column fracture of acetabulum without involvement of true pelvic brim,high anterior column fracture of acetabulum without involvement of true pelvic brim with posterior wall fracture of acetabulum and commminuted high anterior column fracture of acetabulum without involvement of true pelvic brim according to present and unpresent of posterior wall fracture.Five cases suffered isolated fracture and 2 cases associated smaller and nondisplacement fracture fragment of posterior wall were reduced and fixed by buttress plate of iliac crest and lag screw of anterior border of ilium through an iliofemoral approach; 4 cases with displacement posterior wall fracture of acetabulum were reduced and reconstructed by buttress plate and lag screws of through combinations of anterior and posterior approaches.One case suffered comminuted fracture were reduced and reconstructed by plates and screws of through extended iliofemoral approach.ResultsThe mean follow-up time of all patients was 26.7 months (range,14-37months).The quality of reduction was grade as anatomical in 8 patients,imperfect in 3,poor in 1 by Matta's score system.The fracture union was uneventful.There was nonunion and loss of internal fixation.At the final follow-up,the mean score was 16.8 (range,11-18),7 cases were graded as excellent,4 good,1 fair,according to modified Merle d'Aubigne and Postel score system.Heterotopic ossification and traumatic osteoarthritis were recorded in 1 patient.ConclusionGood clinical results can be achieved by anatomical reduction and rigid fixation through optimal approach.