1.Effect of Notoginsenoside on Cerebral Infarcted Neurotic Dysfunction
Yiju SHI ; Guoqing XING ; Qinghua MENG ; Jilan PANG ;
Chinese Traditional Patent Medicine 1992;0(10):-
Objective: To study the effect of notoginsenoside in various doses on local ischemic neurotic dysfunction and the ultrastructure. Methods: 35 Wistar rats were divided at random. The reversible middle cerebral artery occlusion (MCAO) model was made according to the assessment of the literature. The changes of SEP contents of cerebral cortex NO and SOD, and the changes of the ultrastrcture were recorded by evoked potential electrograph before and after MCAO. Results: notoginsenoside 200mg?kg -1 and 400mg?kg -1 could remarkably alter MCAO, shorten the latent period, improve the dysfuntion induced by MCAO, lower the concentration of NO and enhance the activity of SOD. The differential value in MCAO group was statistically remarkable ( P
2.Construction and immunogenicity analysis of antigenic epitopes of swine influenza virus.
Huili LIU ; Jilan XING ; Jie PAN ; Qiufeng YANG ; Yanmin ZHAO
Chinese Journal of Biotechnology 2008;24(4):690-694
Several antigen epitopes were designed according to the sequences of Swine influenza virus hemagglutinin (HA) genes and lined with the interval. The gene was amplified by PCR and sub cloned into pET30a (+) vector. The fusion protein was expressed in E. coli BL21 (DE3) by induced with IPTG and purified by affinity chromatography. The molecular weight of the protein was about 20 kD in SDS-PAGE. Immunological activity of the fusion protein was analyzed by Western blot. The results showed that the fusion protein could interact with anti-His antibody and the rabbit antiserum against SIV. The immunized mouse can produced antibodies against the target peptide and HI antibody against SIV H1N1 or H3N2. This study provides a new vaccine candidate for SIV.
Amino Acid Sequence
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Animals
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Antibodies, Viral
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blood
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Antigens, Viral
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biosynthesis
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genetics
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immunology
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Base Sequence
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Epitopes
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genetics
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immunology
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metabolism
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Escherichia coli
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genetics
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metabolism
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Hemagglutinins
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genetics
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immunology
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Humans
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Immunization
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Influenza A Virus, H1N1 Subtype
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genetics
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immunology
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Influenza A Virus, H3N2 Subtype
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genetics
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immunology
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Random Allocation
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Recombinant Fusion Proteins
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biosynthesis
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genetics
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immunology