1.The protective effects of scallop skite-glycosaminoglycan on vascular endothelial cell injury induced by OX-LDL
Junling ZHANG ; Sai LIU ; Ruichen WANG ; Jiju HAN
Chinese Pharmacological Bulletin 2003;0(12):-
Aim To study on the protective effects on vein endothelial cell of scallop skite-glycosaminoglycan(SS-GAG)and the mechanism of anti-atherosclerosis action of SS-GAG.Methods The endothelial cell of human umbilical vein had been cultured in vitro, and we established an model of endothelial cell oxidative damage induced by oxidized low density lipoprotein (OX-LDL), MTT assay and chemical methods were used to test the influence of SS-GAG on proliferation activity of endothelial cell oxidative damage and analyze nitric oxide (NO) and endothelial nitric oxide synthase (eNOS).Results Oxidized lowdensity lipoprotein (OX-LDL) remarkably inhibited the ability of cell proliferation, decreased nitric oxide (NO) and endothelial nitric oxide synthase (eNOS) (P
2.Inhibitory effects of propolis on platelet adhesion in flowing blood in vitro
Yunxiang ZHANG ; Wei LI ; Yanhua PAN ; Jiju HAN ; Yaping WU ; Jiafu WANG
Chinese Journal of Pathophysiology 1986;0(02):-
AIM:To investigate effects of propolis-ethanol extract on platelet activity in flowing blood.METHODS: Intima-injury model was made by in vitro perfusion.Coverslips was coated by using fibrinogen and human Ⅲ type collagen to detect platelet adhesive rate when blood went through fibrinogen and collagen surface in 1 000/s shear rate.Three experimental groups were set up: negative control group(24% ethanol),experimental group(0.1 g/L,24% propolis-ethanol extract) and positive control group(0.1 g/L,ferulic acid).RESULTS: Intima-injury model was made.On fibrinogen and collagen,platelet adhesion on area of experimental group was lower than that in negative control group(P0.05).Propolis-ethanol extract decreased more obviously the platelet adhesion area on fibrinogen surface than that in collagen surface(P
3.Development and clinical application of vWF-pp ELISA Kit
Jiju HAN ; Daoling REN ; Bin CHEN ; Zengxian WANG ; Ran WEI ; Yaping WU
Chinese Journal of Laboratory Medicine 2001;0(03):-
Objective To establish a sandwich enzyme linked immunosorbent assay (ELISA) method for determination of von Willebrand factor propeptide (vWF-pp) in plasma and to determine the levels of plasma vWF-pp in 152 healthy volunteers, 36 patients with coronary heart disease and 38 cases of the normal control group with the same ages.Methods The levels of vWF-pp in 152 healthy volunteers with different ages and different genders and in 36 patients with coronary heart disease were determined with the sandwich enzyme linked immuno-sorbent assay (ELISA).Results The average level of vWF-pp in 152 healthy volunteers was 496.4?167.2 ?g/L.The level of vWF-pp in the 50~60 years old group was significantly higher than that in the less than 40 years old group (P0.05). There was significant difference between the 51~60 years old male group and the other three male groups respectively, the 16~20 years old group, the 21~30 years old group and the 31~40 years old group (P