AIM　To study the roles of non-genomi c mechanism of glucocorticoid in the integration of sympathetic nervous system. METHODS　The spontaneous discharge of the identified cardiovascula r neurons in the rostral ventrolateral medulla (RVLM) were extracellularly recor ded in urethane-anaesthetized rats. The effects of microiontophoresis of cortic ostersone sulfate (CORT) on the discharge of the cardiovascular neurons in the RVLM were observed. The responses of RU 486 (a blocker for cytosolic glucocortic oids) to the effects of CORT on the cardiovascular neurons were investigated. RESULTS　Totally 33 cardiovascular neurons in the RVLM were recorded , the firing rate of 25 (76%) cardiovascular neurons increased by microiontophor esis of CORT. The effects of CORT were also positively correlated with the curre nt. In 8 (24%) cardiovascular neurons, microiontophoresis of CORT had no effect on their spontaneous discharge. In 12 of 33 cardiovascular neurons, which discha rge increased by CORT, microiontophoresis of RU 486 had no responses to the base line discharge of these cardiovascular neurons (P>0.05), but completely (3 neurons) or partially (9 neurons) blocked the excitatory effect induced by CORT. CONCLUSION　CORT had rapid excitatory effects on the cardiovascul ar neurons in the RVLM. RU 486 had no responses to the baseline activity of the cardiovascular neurons, and but completely or partially blocked the effect of CO RT on the cardiovascular neurons.

0.05), but completely (3 neurons) or partially (9 neurons) blocked the excitatory effect induced by CORT. CONCLUSION CORT had rapid excitatory effects on the Caldiovascular neuronsin the RVLM. RU 486 had no responses to the baseline activity of the cardiovascular neurons, and but completely or partially blocked the effect of CORT on the cardiovascular neurons.

Objective: To study the role of glucocorticoid i n the integration of sympathetic nervous system and cardiovascular activity. Methods: Neurons in the rostral ventrolateral medulla (RVLM) were extracelluarly recorded and identified as the presympathetic neurons of adult rats. The spontaneous discharge of the presympathetic neurons in the RVLM were observed by bolus intravenous injection of corticosterone (50, 100, 150 μg/kg） . Results: The firing rate of 12 presympathetic neurons was incr eased by intravenous application of corticosterone (P<0.05), and this effect showed a dose-dependent manner. The latency of excitatory effect was (104±2 5) s. Conclusion: Corticosterone can rapidly excite the presym pathetic neurons in the RVLM, this action might be involved in the integration o f sympathetic nervous system through the “rapid membrane effects”.

Objective To investigate risk factors for healthcare-associated infection(HAI)in patients with craniocerebral operation,and provide reference for the prevention and control of HAI.Methods A total of 4 246 cases of craniocerebral surgery in a neurosurgery department from January 2010 to December 2012 were enrolled in the study,the risk fac-tors for HAI were analyzed.Results Of 4 246 cases of craniocerebral surgery,393 patients developed 446 times of post-operative HAI,HAI incidence rate was 9.26%,case infection rate was 10.50%,which were higher than inci-dence (2.02%)and case infection rate (3.02%)of HAI of all hospital during the same period,the difference was statistically significant (χ2 =811 .06,629.30,respectively,P <0.001).The major infection site was central nervous system (56.50%),followed by respiratory system (27.36%).Unconditional univariate logistic regression analysis showed that sex,age,community-acquired infection,primary disease,operative time,length of stay in intensive care unit (ICU)before infection,the number of surgery,invasive procedures and nasogastric tube,the difference was statistically significant (all P <0.05 ).Multivariate logistic regression analysis showed that sex,elderly pa-tients,congenital brain diseases,stay in ICU>7 d,antimicrobial use >7 d,central venous and urinary tract cathe-terization,invasive ventilator were risk factors for HAI in patients with craniocerebral operation.Conclusion The incidence of HAI in patients with craniocerebral operation is high,effective preventive and control measures accord-ing to risk factors should be strengthened to reduce the incidence of HAI.

The long-period CT perfusion imaging leads to an excess amount of radiation dose to the patient. However, the radiation dose could be significantly reduced if a previous normal-dose image is acquired before a set of low-dose scans of perfusion, and a filtering processing is performed on the differences between the current low-dose images and the previous normal-dose image, then the results are added to the previous image. But the selection of plenty of parameters makes the algorithm complicated. This paper proposes an innovative approach performed in sinogram domain instead of in image domain. First a normal-dose image and a set of low-dose projection data are acquired before the perfusion. Second the perfusion information is commendably reconstructed with sparsity constraints of the differences between current low-dose perfusion sinograms and previous low-dose sinogram. Finally, the reconstructed perfusion information is added to the previous normal-dose image. The proposed method was validated by simulated experiments with a set of brain CT perfusion images, which showed that the new method provided more accurate perfusion information; the time-attenuation curve was more close to that for normal-dose scan and the mean transit time more repeatable.
Algorithms ; Brain ; diagnostic imaging ; Humans ; Perfusion Imaging ; methods ; Radiation Dosage ; Radiation Injuries ; etiology ; prevention & control ; Radiographic Image Interpretation, Computer-Assisted ; Subtraction Technique ; Tomography, X-Ray Computed ; adverse effects ; methods

Objective To analyze the change of cigarette consumption price,and understand the cigarette affordability in China.Methods A total of 16 800 households were selected through multi-stage stratified cluster sampling.Then IPAQ was used to randomly select one family member to conduct the survey.Questionnaire from Global Tobacco Surveillance System with added country-specific questions was used.Results Up to 50％ of current smokers would buy 20 cigarettes with price of 9.9 yuan (RMB) or less,and 25％ of current smokers would not buy 20 cigarettes with price exceed 5.5 yuan (RMB).Only 10％ would buy 20 cigarettes with price over 19.9 yuan (RMB).The calculated median monthly expenditure for cigarettes was 181.4 yuan (RMB).From 2010 to 2015,the proportion of annual expenditure for cigarettes in disposable income per capita declined from 10.5％ to 8.8％ in urban area and from 21.1％ to 17.3％ in rural area.Conclusion During 2010-2015,the purchasing power of Chinese smokers increased in both urban area and rural area due to the decrease of cigarette consumption price.

Objective:To probe the exploration and integration of ideological and political teaching resources for College English by medical students' teams, and to build a College English ideological and political teaching resource database in medical colleges, so as to provide feasible experience for the integration of medical students' College English and ideological and political education.Methods:A questionnaire survey was conducted among 1 030 students in a medical college, and SPSSAU statistical software was employed to analyze the results to investigate the attitudes and demands of students towards the integration of English teaching and ideological and political education, the construction methods of College English ideological and political teaching resource database, and the presentation methods and types of resources. Based on the questionnaire results, textbook themes, teaching modules and students' majors, the content analysis and classification method were employed to construct a College English ideological and political teaching resource database that meets the expectations of medical students.Results:① A total of 836 students agreed with the necessity to integrate College English with ideological and political education, hoping to build an ideological and political resource database with videos and audios related to daily life ( n = 731), medical knowledge ( n = 664), social practice ( n = 622), news and current affairs ( n = 604) and guide the students to learn the above resources through the methods "teacher instruction + classroom discussion" ( n = 528). ② The ideological and political integration points in the questionnaire results were matched according to the subjects of the textbook, and further divided into two categories: universal and medically specific ideological and political teaching elements according to the content of the unit modules. Finally, the resource database of College English ideological and political teaching in medical colleges was built. Conclusion:Outstanding courses and resources are jointly explored and built by teachers and students.

Objective To understand the situation on exposure to second-hand smoke among Chinese adults aged 15 and above and their support to policy on banning smoking in public places in 2015.Methods A cross-sectional survey was conducted based on the protocol on Global Adult Tobacco Survey.Stratified multi-stage cluster sampling was used to select 16 800 households in China.Data were collected through household survey,using the electronic devices.One eligible respondent was selected by random sampling in each household.Standardized questionnaire was used from Global Tobacco Surveillance System.Data were weighted and analyzed by SAS 9.3 and SUDAAN 10.0.1 complex survey data analysis program.Results 93.1％ (60 128 × 103/64 575 × 103),76.3％(455 473 × 103/596 782 × 103),57.1％ (621 793 × 103/1 089 582 × 103),54.3％ (218 792 × 103/402 732 × 103),38.1％ (61 208 × 103/160 574 × 103),26.9％ (119 783 × 103/444 679 × 103),23.8％(17 598 × 103/73 993 × 103),17.2％ (41 099 × 103/239 022 × 103) and 16.4％ (102 153 × 103/623 015 ×103) of the respondents reported that smoking did exsit in the following places as:inside of bars or night clubs,in the restaurants,households homes,working places,government buildings,health care facilities,universities,primary and high schools (both indoor and outdoor areas),and on public transportation,respectively.Compared with those data in 2010,the proportions of exposure to indoor areas of restaurants,government buildings,health care facilities,primary and high schools (both indoor and outdoor areas),public transportation,and homes all significantly decreased after standardization of data.Results also showed that most respondents were supportive to the policy on banning smoking in public and working places.However,the proportions of exposure to indoor areas of restaurants were still high but almost 70％ of the respondents thought smoking should not be allowed in indoor areas of restaurants.Conclusion From 2010 to 2015,proportions of exposure to indoor areas of public places,working places,public transportation and homes were obviously declining in China.The Chinese public seemed supportive to the policy on banning smoking in public places.

Objective To evaluate the performance of large language models in answering ques-tions about childhood asthma,comprehensively understand the quality of their provision of information on children's health,and identify their limitations to facilitate model improvement.Methods Sixty common questions related to childhood asthma were formulated and put to two large language models known as Wenxin Yiyan and Zhipu Qingyan,which were publicly available in China.Three pediatric asthma specialists assessed the quality of the large language models'responses by using a blind meth-od.Results Wenxin Yiyan scored higher in terms of accuracy,understanding,reliability,and logi-cality;Zhipu Qingyan scored higher in term of safety.Comparing the scores of the five different di-mensions,it was found that large language models scored higher in terms of understanding,reliability and logicality,but relatively insufficient in terms of accuracy and safety.Conclusion Application of large language models in the education of children with asthma can provide useful references for asth-ma children and their parents.However,the current large language model technology still has certain limitations in terms of accuracy and safety,which requires further improvement and optimization.

Objective To evaluate the performance of large language models in answering ques-tions about childhood asthma,comprehensively understand the quality of their provision of information on children's health,and identify their limitations to facilitate model improvement.Methods Sixty common questions related to childhood asthma were formulated and put to two large language models known as Wenxin Yiyan and Zhipu Qingyan,which were publicly available in China.Three pediatric asthma specialists assessed the quality of the large language models'responses by using a blind meth-od.Results Wenxin Yiyan scored higher in terms of accuracy,understanding,reliability,and logi-cality;Zhipu Qingyan scored higher in term of safety.Comparing the scores of the five different di-mensions,it was found that large language models scored higher in terms of understanding,reliability and logicality,but relatively insufficient in terms of accuracy and safety.Conclusion Application of large language models in the education of children with asthma can provide useful references for asth-ma children and their parents.However,the current large language model technology still has certain limitations in terms of accuracy and safety,which requires further improvement and optimization.