1.Tubulin expression of basal cells in patients with Alzheimer's disease and vascular dementia
Jihua XIAO ; Chaoyong XIA ; Yanbin JIA ; Pei ZHENG
Chinese Journal of Pathophysiology 2000;0(07):-
AIM: To investigate quantitatively the tubulin expression of basal cells in patients with Alzheimer's disease(AD) and vascular dementia(VD) and evaluate its significance in diagnosing Alzheimer's disease. METHODS: ?-tubulin of basal cells was examined using LSAB immunohistochemical technique, and analyzed qualitatively and quantitatively by image analyzer. RESULTS: The integrated absorbance (I A ,261.43?25.21) and the average absorbance (A A ,1.89?0.14) in the AD group were significantly higher than those (I A ,120.55?19.71 and A A , 0.85?0.14, respectively)in the VD group ( P
2.The analysis of genetic polymorphisms and haplotype of the Trail gene in the patients with ulcerative colitis
Yi JIANG ; Jihua PEI ; Limiao LIN ; Changlong XU ; Jianzhang WANG ; Sujian SHEN ; Zhanxiong XUE
Chinese Journal of Digestion 2011;31(12):812-816
Objective To explore the association between genetic polymorphisms and haplotypes of tumor necrosis factor-related apoptosis inducing ligand (Trail) and ulcerative colitis (UC).Methods A total of 331 patients with UC and 832 age and sex-matched healthy controls were collected.After Trail gene was amplified by PCR,the genetic polymorphisms of three single nucleotides (G1525A/G1588A/C1595T) in 3' non coding regions of Trail gene were examined by direct sequencing.The relation between Trail haplotype and UC was analyzed.Results Compared with control group,the frequencies of variant allele A and genotype GA+ AA in Trail G1525A were significantly lower in UC group (both P<0.01).The frequencies of variant allele A and T in Trail G1588A and C1595T were also significantly lower in UC group than that of control group,and the difference was statistically significant (both P < 0.01 ).In mild and moderate UC patients,the frequencies of variant allele T and CT+TT in Trail C1595T were 49.15% and 64.51%,in severe UC patients were 72.37% and 84.21%,and the differences were significant between the two groups (OR=2.710 and 2.935,95%CI:1.598~4.596 and 1.188~7.249,all P <0.05).In severe UC patients,the frequency of variant allele A in Trail G1525A was 48.69%,which was higher than that of mild and moderate patients (35.16%,OR=1.750,95%CI:1.082~2.830,P=0.021).In UC group,the frequency of AAT haplotype was significantly lower than that of controls (43.09% vs 58.41%,P<0.01).The frequency of GAT haplotype was significantly higher in UC group (10.15%vs 0.18%,95% CI:0.005 ~ 0.051,P< 0.01).Conclusion The genetic polymorphisms and haplotypes of Trail (G1525A/G1588A/C1595T) gene may be closely correlated with the susceptibility to UC.
3.Association of vitamin D receptor gene polymorphisms and haplotypes with Crohn ' s disease
Jihua PEI ; Hao WU ; Xiuqing LIN ; Jie JIN ; Ran DING ; Yi JIANG
The Journal of Practical Medicine 2017;33(5):735-738
Objective To analyze the association of Crohn's disease(CD)with vitamin D receptor(VDR) gene polymorphisms. Methods After collecting 326 CD patients and 464 healthy controls,the four single nucleotide polymorphisms of VDR (FokI, BsmI, ApaI and TaqI) were examined by a SNaPshot technique. Results Compared with those in controls,the frequencies of mutant allele(A)and genotype(GA+AA)of BsmI were significantly decreased in CD patients(both P=0.001). The similar conclusions were also drawn for the mutant allele(C)and genotype(TC+CC)of TaqI(both P<0.05). In further stratified analysis,compared with those in controls,the mutant alleles and genotypes of BsmI and TaqI were significantly reduced in stenotic type CD patients (all P<0.0083). The analyses of linkage disequilibrium(LD)and haplotype showed that BsmI,ApaI and TaqI were in a strong LD,and the formed haplotype AAC was significantly lower in CD patients than that in controls (P <0.05). Conclusions VDR(BsmI and TaqI)polymorphisms are significantly related with the reduced susceptibility to CD,especially for patients with stenotic CD. Moreover,the haplotype AAC might engender a reduced risk of CD.
4.Association of vascular endothelial growth factor gene polymorphisms with Crohn's disease among Chinese patients.
Jihua PEI ; Liqin YU ; Xuanping XIA ; Shenglong XIA ; Xiuqing LIN ; Jie JIN ; Yi JIANG
Chinese Journal of Medical Genetics 2018;35(4):582-586
OBJECTIVETo assess the association of vascular endothelial growth factor (VEGF) gene polymorphisms with susceptibility to Crohn's disease (CD) in a Chinese population.
METHODSFor 275 CD patients and 495 controls, the genotypes of VEGF gene rs699947 and rs3025039 loci were determined with a SNaPshot method.
RESULTSThe allelic and genotypic frequencies of the rs699947 and rs3025039 loci did not differ between the two groups (all P>0.05). By stratification analysis, allele A and genotype CA+AA of rs699947 were more frequent in patients with colonic CD compared with the controls (P=0.006, 95%CI:1.143-2.234; P=0.005, 95%CI:1.203-2.900, respectively). Compared with the controls, the allele A and genotype CA+AA of rs699947 were less frequent in patients with ileal lesions including ileal CD and ileocolonic CD (P=0.033, 95%CI:0.524-0.974;P=0.043, 95%CI:0.481-0.989, respectively). The frequency of TT homozygote of rs3025039 was lower in patients with non-stricturing and non-penetrating CD compared with the controls (P=0.036, 95%CI:0.016-0.870).
CONCLUSIONPolymorphisms of the VEGF gene rs699947 locus may contribute to an increased risk for colonic CD, but may play a protective role in patients with ileal lesion. Individuals carrying the TT genotype for VEGF rs3025039 locus may be less susceptible to non-stricturing and non-penetrating CD.