Object To establish a tissue culture and rapid propagation system for the medicinal plant Iphigenia indica Kunth. Methods Clusters of seedlings and protocorms and induction were studied on MS media with different parts of the plant, such as corms, stems, leaves and root tips as explants. Results The proper media for protocorm inducing, propagation and rooting were found in this paper, rapid propagation system of I. indica was established and the plant could put in large scale production. Conclusion Sprout can be successfully induced from corm and stem on MS media. MS+6-BA 2 mg/L+NAA 0.5 mg/L can induce both sprout and protocorm, and fit for large scale production. 1/2 MS+IBA 0.5 mg/L+NAA 0.2 mg/L is good for rooting.

Objective To investigate the signal regulatory mechanism of expression of corticotropin releasing hormone (CRH) stimulated neurons of hypothalamic slices in rats in vitro . Methods Model of hypothalamic slices of rats was established. After CRH stimulatation of corticotropin releasing hormone type 1 receptor (CRH1R) of hypothalamic slices in rats in vitro , the changes of activity of protein kinase A (PKA) signal passway were observed by immunocytochemical method and Western blotting. The relationship between the changes and CRH mRNA expression was also observed. Results CRH could cause the remarkable increase in phosphorylated PKA, phosphorylated CREB, and CRH content in hypothalamic slices in rats. However, CPl54526 or H89 could have significant inhibitory effect on the synthesis of P PKA, P CREB, and CRH. Conclusion PKA signal passway can regulate the ultrashort positive feedback of CRH secretion in the rat hypothalamus in stress due to severe trauma.

Objective To study the electrophysiologic characteristics of atrial-ventricular reentrant tachycardia(AVRT)characterized by paroxysmal occurrence that slow atrioventricular accessory pathway participatesin.Methods Twenty-one cases were chosen from patients receiring radiofrequency ablation therapy in Peking University People's Hospital from July in 1999 to January of 2005.The patients with slow atrioventricular accessory pathways diagnosed correctly were divided into two groups with paroxysmal tachycardia and permanent tachycardia in terms of the occurrent frequence of AVRT.The electrophysiologic features of AVRT of two groups were contrastively analyzed.Results Compared with the group of permanent AVRT,it was found that antidromic refractory period of slow atrioventricular accessory pathways was longer[(359?46)ms vs (318?31)ms,P

Objective To investigate the protective effect of transfecting bone morphogenetic protein-7 (BMP-7) on cultured neonatal rat cardiomyocytes against hypoxia-reoxygenation injury. Methods Neonatal rat cardiomyocytes were cultured. The pcDNA-rrBMP7 was introduced into cardiomyocytes by Fugene 6.0 transfection method. The cardiomyocytes were divided into three groups: control group (group C), hypoxia-reoxygenation group (group HR) and gene transfecting group (group BT). Trypan blue exclusion test was performed to detect cell viability. The activity of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) was assayed to evaluate cell injury. For evaluating the cell antioxidant ability, the activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) were determined by colorimetric assay. Fluo-3 labeling method and confocal laser scanning microscopy were used to observe the change in intracellular calcium. Results The results showed that after 120 min of simulated ischemia followed by 240 min of reperfusion, cell pulsation rate was decreased, the activity of LDH, CPK and the trypan blue uptake rate were increased. As compared with the group C, SOD activity decreased and the content of MDA increased in Group HR. Compared with Group HR, the SOD activity increased and the content of MDA decreased in group BT. Treatment with BMP-7 gene transfecting led to a decrease of i content in cardiomyocytes, showing that overloading of i induced by hypoxia-reoxygenation was prevented (P

Objective\ To evaluate the efficacy of atrial lead implantation during atrial fibrillation.Methods\ In 10 patients(group A),the atrial leads were implanted during atrial fibrillation and the sensing and pacing parameters before and after restoring to sinus rhythm were compared with the parameters of patients whose atrial leads were implanted during sinus rhythm (group B).Results\ During follow-up,the sensing and pacing parameters were measured,and there were no significant difference between group A and B.In group A,mean atrial potential amplitude during atrial fibrillation was correlated with the telemetered atrial potential amplitude during sinus rhythm.Conclusion\ Atrial lead implantation during atrial fibrillation is feasible.

Objective Accelerated junctional rhythm (AJR) always occur during slow pathway catheter ablation for atrioventricular nodal reentrant tachycardia (AVNRT), the clinical significance of it has not been gotten in agreement. The aim of this study is to search for an association between AJR and ablation target site or tachycardia recurrence.Methods The data of 247 patients with AVNRT who received radiofrequency ablation procedure during April 1995 to October 1999 was analyzed. All these people were divided into two groups (212 patients in the successful ablation group or group 1, 35 patients in the recurrence group or group 2). The AJR was divided into two distinct pattern:type Ⅰ(continuous AJR that persisted until the end of energy delivery) and type Ⅱ (intermit AJR alternated with sinus rhythm during slow pathway ablation, which was eliminated immediately when stopping energy delivery ). Results\ The results showed that patients in group 1 exhibited better AJR response, most of them were seen with type Ⅱ AJR. However most of the people in group 2 had no AJR response throughout energy delivery , few of them had type Ⅰ AJR response. The AJR response of group 1 started relatively earlier than that of group 2(3 2?1 8 vs 5 7?2 5 ,P

Objective The aim of our study is to differentiate left atrial from right atrial tachycardia on the basis of P wave configuration during focal atrial tachycardia (AT).Methods Thirty-three patients with focal atrial tachycardia who had undergone successful radiofrequency catheter ablation were considered for inclusion in the study . Site of origin was the left atrium in 10 patients, and right atrium in 23 patients . The surface 12-lead ECG recordings during atrial tachycardia were reviewed in all patients. We analyzed the correlativity between P wave configuration and left atrial tachycardia.Results The specificity of negative P wave in lead I and aVL that indentifed a left atrial focus was 100% and 95% respectively, but the sensitivity was only 30% and 50% respectively. The specificity and sensitivity of positive P wave in V 1 that predicted a left atrial focus was 87% and 80%,respectively.Conclusion It is possible to predict the site of origin of focal atrial tachycardia by analyzing its P wave configuration,,and may be helpful for preparation before ablation.

1.9?V and 95% confidence interval was 1.52-2.41?V.There was significant differences among the groups(P

Objective To investigate the association between syncope and transient suppression of sinoatrial node in patients with preexcitation syndrome and paroxysmal supraventricular tachycardia (PSVT).Methods Twelve patients (male 8,female 4;age range:19-49 years) were enrolled due to WPW(Wolff-Parkinson-White) syndrome and syncope when the tachycardia terminated.The function of sinoatrial node was evaluated with clinical electrophysiologic study,and then radiofrequency catheter ablation (RFCA) or antitachycardia pacemaker were optimized for the patients with a follow-up of 2 to 5 years.Results Electrocardiographic features of the 12 patients were: ventricular preexcitation (type A in 7 and type B in 5) and long pause when tachycardias ended up.Electrophysiologic study revealed the function of sinoatrial node was normal.Four patients received antitachycardia pacing therapy,5 underwent RFCA,and 3 received both.The 2 to 5 years follow-up found no relapse of PSVT associated syncope.Conclusion Transient suppression of sinoatrial node might explain the mechanism of syncope with preexcitation and PSVT.However,the reason why such a small group of patients differ from the majority of preexcited cases in clinical course remains to be unsettled.The first choice of prevention and treatment for these patients should be eliminating PSVT.Conventional pacemaker implantation for preventing syncope seems to be unnecessary.

Objective To investigate the development and metastasis of malignant choroidal melanoma cell strain OCM-1-gfp modified with green fluorescent protein(GFP) and the factors which affected the tumor biological behaviors. Methods GFP was transfected into malignant melanoma cell strain OCM-1.Melanoma cells with high and stable expression of GFP were injected into subretinal space and the subcutaneous space of hind leg of Balb/c nude mouse respectively in order to establish orthotopic and heterotopic transplanted tumor models.The development and metastasis process of orthotopic tumor models was observed directly by fluorescence microscope,and the size of the hypodermal tumor was measured by vernier.The expressions of 13 genes in melanoma were detected by means of immunohistochemistry staining. Results Malignant choroidal melanoma cell strain OCM-1 stably expressed GFP and preserved the characteristics of parental generation,OCM-1-gfp may develop melanoma and continue to metastasize in nude mouse.Positive expression of most of the antibodies,including Rb,p53,p21,E_2F,NF?B,cyclin D1,proliferation cellular nuclear antigen(PCNA),bcl-2、bcl-XL/S,bax,and epithelial growth factor(EGF)and its receptor(EGFR),was found.While the staining of inhibition gene p16 was negative. Conclusions GFP is the marker for observing the development and metastasis of malignant choroidal melanoma in vivo.The rate of tumor formation and development process in orthotopic models does not differs much from which in heterotopic models of malignant choroidal melanoma.The expressions of lots of genes in malignant choroidal melanoma developed from OCM-1-gfp including p16、p53、NF?B,cyclin D,PCNA,EGF,and EGFR are abnormal.