BACKGROUND: The problems such as fast drug degradation, great drug loss and poor barrier effect exist when using liquid molecular biomaterial as barriers in preventing tendon adhesion. Accordingly, it has aroused increasing attention to seek for membrane biomaterials as barriers. Simultaneously, it found that tendon cells would proliferate and differentiate under controls of multiple endogenous growth factors that promote tendon endogenous healing. However, it is poorly understood which the specificity factor for tendon healing is. OBJECTIVE: To study effect of epidermal growth factor (EGF) combined with degradable collagen membrane on preventing tendon adhesion and improving tendon endogenous healing. METHODS: Thirty ten-month old leghorn cocks were randomly divided into 3 groups, with 10 animals in each group. The third toe of left foot of each animal was prepared for avulsion model, and sutured with improved Kessler method. The broken ends were encapsulated with EGF combined with degradable collagen membrane (combination group), degradable membrane alone (collagen membrane group) or without treatment (blank control group). Four weeks later, the specimens were evaluated by gross observation, biomechanical test, light microscope and electron microscope. RESULTS AND CONCLUSION: In the combination group, there were a large amount of type Ⅰ collagen inside the sutured tendon, they were closely and lined up in order. The amount of collagen-fibronectin was less and the adhesion obviously was less than the control group. The tendon cells were matured. The adhesion in the collagen membrane group was slightly, there were a large amount of type Ⅲ collagen inside the sutured tendon, which loosely but well organized. In the blank control group, type Ⅰ and Ⅲ collagen arranged crisscrossed, with heavy adhesion. The results suggest that EGF can promote tendon endogenous healing and degradable collagen membrane can prevent tendon exogenous healing, thus, prevent the formation of adhesion.

Antiviral Agents ; therapeutic use ; Drug Combinations ; Hepatitis C, Chronic ; drug therapy ; psychology ; Humans ; Interferon-alpha ; therapeutic use ; Polyethylene Glycols ; therapeutic use ; Recombinant Proteins ; therapeutic use ; Ribavirin ; therapeutic use

BACKGROUND:As mechanical load-bearing tissues, tendons have unique biomechanical characteristics. Mechanical loading is necessary in tendon development, and the tendon can alter its structure and biological behaviors in response to the various mechanical loading conditions. OBJECTIVE: To fuly understand the healing process and biomechanical properties of the damaged tendon so as to know the researching progress in the role of stress in the tendon healing process. METHODS: An electronic search of Chinese Biomedical Literature Database and PubMed databases was done for colection of reviews and papers addressing stress effects on tendon healing, and then we analyzed the role of stress in the healing process of tendon from the micro and macro levels. RESULTS AND CONCLUSION:Totaly 59 relevant articles were enroled. Tendon is sensitive to stress, and it can change its structure and biological reaction in response to different stress loadings. Proper stress stimulus to the tendon is necessary to the tendon development and healing. How to achieve a good balance between the lowest (resulting in alienation effect) and the highest stress loadings (resulting in minimaly invasive injury) during the clinical tendon healing is a chalenge. At present the treatment of tendon injuries is stil a huge chalenge to clinicians, and the vast majority of tendon injuries belong to tissue healing.

BACKGROUND:Tissue-engineered tendons have been used to repair the damaged tendon tissue. Use of tissue-engineered tendons for repair of tendon injury has become a hot spot in this research field. OBJECTIVE: To elaborate the types, advantages and disadvantages of seed cels, the design method, advantages and disadvantages of scaffold materials, and the factors that induced the formation of tendon, so as to promote the optimization of each joint, al of which benefit for mature construction of tissue-engineered tendons. METHODS: The related reviews and paper reports of tendon tissue engineering published from January 2000 to January 2015 were retrieved from Chinese Biomedical Literature Database (CBM), China Knowledge Resources Database (CNKI) series database, Chinese Citation Database and PubMed database. The key words were “tissue engineering; tendon; tendon defect”. The research progress of seed cels, scaffold material and induction factors were analyzed. RESULTS AND COMCLUSION:The recent research of tissue-engineered tendons for repair of tendon injury has been summarized. Seed cels, scaffold, induction factors were discussed. Tendon stem cels, as a kind of seed cels, are currently the first choice in the process of tissue engineering tendon research, because tendon stem cels have the homology of the homogenous or autologous tendons and possess strong differentiation and proliferation capacities. However, there have been no systematic schemes regarding acquisition and proliferation and culture of tendon stem cels. The currently designed tissue-engineered tendons cannot meet the clinical requirements because of poor mechanical properties of tendon tissue, poor integration with the host tissue, being susceptible to degradation in late period and functional disuse. Induction factors are the laft key factors for tissue-engineered tendons for repair of tendon injury. The selection and use of induction factors are prerequisites for the regulation of tendon tissue development. But the categories of induction factors and the association and interrelationship between induction factors have not been fuly clear and studies are needed to further investigate these uncertainties.

Objective To investigate the imaging features and preoperative staging value of ultrasonic endoscopy and gastrointestinal ultrasonic contrast in the gastric carcinoma examination.Methods One hundred and sixty eight patients with gastric carcinoma verified by operation and pathology in our hospital from October 2010 to October 2015 werecollected.Then their imageological data including ultrasonic endoscopy and gastrointestinal ultrasonic contrast examinations were retrospectively analyzed and compared with the postoperative pathological results.Results The accuracy rate of preoperative T staging in ultrasonic endoscopy was significantly higher than that of gastrointestinal ultrasonic contrast,the difference had statistical significance(P＜0.05);the accuracy rate of preoperative N/M staging in gastrointestinal ultrasonic contrast was s1gnificantly higher than that in ultrasonic endoscopy,the difference had statistical significance(P＜0.05).Conclusion Ultrsonic endoscopy and gastrointestinal ultrasonic contrast have different advantages and values in preoperative staging:ultrasonic endoscopy has higher accuracy rate in the diagnosis of primary gastric carcinoma;gastrointestinal ultrasonic contrast is better in diagnosing metastasis of gastric carcinoma,moreover which shows the consistency with postoperative pathological staging.

OBJECTIVETo prospectively evaluate the efficacy of a traditional Chinese medicine (TCM)-based therapy for treating liver fibrosis in patients with chronic hepatitis B (CHB), and to investigate the patients' perception of the treatment's effects on quality of life (QoL).

METHODSA total of 430 patients with CHB-related liver fibrosis were randomly assigned to treatment groups for receipt of a 12-month course of the antiviral drug entecavir alone (control group) or in combination with the TCM Liuweiwuling tablets. Patients were assessed before (pre-treatment) and after therapy and the treatment-related differences in clinical manifestations, levels of liver function markers and liver fibrosis indexes, color ultrasound images, and hepatitis B virus (HBV) DNA load were compared between the two groups by statistical analysis. The generic QoL scale developed by the World Health Organization (WHOQOL-BREF) was used to score the patients' perceptions of treatment outcome.

RESULTSAfter treatment, the patients in both groups showed significant improvement in the majority of clinical manifestations (both P less than 0.05), with the exception of bloating. In addition, both groups showed significant improvements of liver function markers and in signs of liver fibrosis (both P less than 0.05). Both groups also showed significant reductions in the diameters of the portal and splenic (both P less than 0.05), as well as increases in the rate of undetectable HBV DNA (with a statistically similar outcome achieved in the two groups). Finally, both groups had higher QoL scores after treatment, with all assessed parameters except environment showing a significant improvement (all P less than 0.05).

CONCLUSIONWhen used in combination with entecavir, the TCM Liuweiwuling tablet is a safe therapy for CHB and its related liver fibrosis and may help to improve the QoL of these patients.

Adult ; Antiviral Agents ; therapeutic use ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Guanine ; analogs & derivatives ; therapeutic use ; Hepatitis B, Chronic ; complications ; drug therapy ; Humans ; Liver Cirrhosis ; drug therapy ; etiology ; Male ; Middle Aged ; Phytotherapy ; Quality of Life ; Treatment Outcome

Asian Continental Ancestry Group ; genetics ; Carcinoma, Hepatocellular ; genetics ; China ; Gene Frequency ; Genetic Predisposition to Disease ; Humans ; Liver Neoplasms ; genetics ; Mongolia ; Polymorphism, Single Nucleotide

BACKGROUND: At present, the basic molecular etiological mechanism and core regulatory network of deep vein thrombosis (DVT) remains uncertain, and there is not an ideal measure for early diagnosis of DVT. OBJECTIVE: To study the underlying impact of cathepsin L/G in DVT rat model. METHODS: DVT rat models (n = 50) were established by clamping both femoral vein in three different positions within 3 seconds with mosquito forceps and fixing with cast. According to different observation phases and biological situations of the femoral vein thrombosis, model rats were divided into thrombogenesis group, pre-thrombogenesis group and non-thrombogenesis group. An additional 10 normal rats served as control group. Femoral vein was obtained at corresponding time points to exact total RNA. After a gene chip-based screening, the data of gene expression were further dissected by real-time PCR. RESULTS AND CONCLUSUON: Gene chip hybridization analysis results demonstrated that differential expression of cathepsin L/G gene was significant among groups, and the expression was greatest in the thrombogenesis group, followed by pre-thrombogenesis and non-thrombogenesis groups, which was significantly greater than the control group (P < 0.05). Real-time PCR analysis results were consistent with gene chip hybridization analysis results. These indicate that DVT is associated with an increase in expression of cathepsin L/G in local venous vascular wall, and they may be candidate molecular markers for early diagnosis of deep vein thrombosis.

Objective To discuss related factors of perioperative pulmonary complications in infants with congenital heart disease and provide a scientific basis to improve the quality of intensive care in in-fants with congenital heart disease after surgery. Methods Tracking survey was carried out in 225 cases of infants with congenital heart disease in our city to observe the perioperative lung condition from March 2005 to May 2007. Every process that might occur pulmonary complications,such as preoperative care of children,anesthesia and cardiopulmonary bypass surgery (CPB) management and postoperative monitoring was closely observed. Statistical analysis was conducted according to emerging problems and summarized the relevant factors and nursing methods. Results 225 infant patients passed the operation with no seri-ous complications.Conclusions Effective treatment and nursing during every process of perioperation was the key factor to reduce pulmonary complications.

To construct the lentiviral vector containing Peroxiredoxin 2(Prdx2) gene and the colorectal cancer cell line stably transduced with Prdx 2-containing vector , so as to provide a useful tool for studying the role of Prdx 2 in colorectal cancer.Methods: Prdx2 was amplified by PCR and inserted into lentiviral expression vector Ubi-MCS-EGFP-IRES-Puromycin (GV218) to generate Ubi-Prdx2-EGFP-Puromycin(LV-Prdx2) vector.The inserted Prdx2 gene was verified by double enzyme digestion and DNA sequencing.Subsequently ,lentiviruses were produced and transduced into SW 480 cells.EGFP expression was examined under fluorescence microscopy ,the expression of Prdx2 was detected with qRT-PCR and Western blot.Cell growth and colony forming ability were detected with MTT and plate cloning technique.Results: The lentiviral Prdx2 expression vector was successful construc-ted.Overexpression of Prdx2 was verified in SW480 cells with LV-Prdx2 vector.Prdx2 promoted SW480 cell growth and colony forming ability(P<0.05).Conclusion:Ubi-Prdx2-EGFP-Puromycin(LV-Prdx2) vector is successfully constructed,and the SW480/LV-Prdx2 cell line with stable transduction of Prdx2 containing vector is established.Overexpression Prdx2 can significantly promote the proliferation of colorectal cancer SW 480 cells.