Object To establish a tissue culture and rapid propagation system for the medicinal plant Iphigenia indica Kunth. Methods Clusters of seedlings and protocorms and induction were studied on MS media with different parts of the plant, such as corms, stems, leaves and root tips as explants. Results The proper media for protocorm inducing, propagation and rooting were found in this paper, rapid propagation system of I. indica was established and the plant could put in large scale production. Conclusion Sprout can be successfully induced from corm and stem on MS media. MS+6-BA 2 mg/L+NAA 0.5 mg/L can induce both sprout and protocorm, and fit for large scale production. 1/2 MS+IBA 0.5 mg/L+NAA 0.2 mg/L is good for rooting.

The paper gives an account of the basic methods for assessing the intrinsic quality of patient nursing by nursing procedures. These include: ①monitoring the accuracy of nursing assessment via evaluating the integrity of patients health; ②evaluating the soundness of nursing diagnosis via analyzing and judging the individuality of patients; ③evaluating the rationality of nursing goals and the appropriateness and operability of nursing measures through patients changes in behavior, function, cognition and emotion; and ④evaluating the timeliness and cyclicity of nursing result assessment through the improvement of patients overall conditions. The paper also analyses the problems involved in employing nursing procedures and countermeasures, pointing out that timely transformation of the nursing management model can markedly enhance the intrinsic quality of nursing.

Cooperation networks of nursing techniques were constructde by way of perspective practice so as to enhance the quality of nursing critical cases, the success rate of emergency treatments and patients' life quality. The measures included training in techniques for nmsing critical cases, making the rounds of wards and consultations, and enlarging the scope ofthe cooperation networks. Quantification methods were introduced into management, networks of critical case assessment were used in managing patients, and the system for assessing training in techniques for nursing critical cases was used in managing nursing personnel, thus improving the quality of the nursing pensonnel, ensuring the rational allocation of human resources and promoting the enhancement of the hospital's medical quality and its social and economic benefits. It is therefore not difficu1t to see that the cooperation networks are of application and dissemination value.

Objective To investigate the application of colposcope in cervical cancer screening in Yunnan ethnic minority areas.Methods The questionnaire survey was conducted on 641 women,and the data of the colposcopy and biopsy were reviewed retrospectively.The colposcopy and pathological diagnosis results were compared.Results The minority women thought colposcopy more acceptable was 78.64％ (383/487).The colposcopy found suspected low-grade cervical lesions were 377 cases,high-grade cervical lesions were 245 cases,cervical condyloma were 15 cases,cervical cancer were 4 cases.The pathological results:278 cases of cervical intraepithelial neoplasia (CIN) Ⅰ,174 cases of CIN Ⅱ,55 cases of CIN Ⅲ,15 cases of cervical condyloma,4 cases of cervical cancer.The consistent rate of colposcopy and pathological examination was 68.17％ (257/377) in low-grade lesions,89.39％ (219/245) in high-grade lesions,cervical cancer and cervical condyloma was entirely consistent.Conclusions The colposcopy is more easily accepted in Yunnan ethnic minority areas,which could be a preliminary diagnosis of cervical lesions,cervical condyloma and cervical cancer.It has an important clinical application value in diagnosis of cervical diseases.

BACKGROUND:Potential mechanisms of scar formation and scar prevention strategies have become more important in current medical research. Scar assessment scales have the potential to contribute to better evaluation of scar properties in both research and clinical diagnosis. OBJECTIVE: To summarize the literatures addressing scar prevention and clinical assessment. METHODS: A computer search of CNKI database from 2000 to 2014, PubMed database from 1975 to 2014 and Foreign Medical Journal Ful-Text Service Database from 2000 to 2014 was performed using the keywords of scar, prevention, treat, cicatrix, assessment, measure in Chinese and English to retrieve articles related to scar prevention strategies and scar assessment scales. Totaly 39 articles meeting the inclusive criteria were included in result analysis. RESULTS AND CONCLUSION: During wound healing, the unbalance between colagen anabolism and katabolism can lead to the formation of pathological scar. Prevention and treatment of pathological scars should be combined, and early intervention was used in wound healing. General scar assessment scales include Vancouver Scar Scale, Visual Analog Scale, Patient and Observer Scar Assessment Scale, Manchester Scar Assessment Scale, Stony Brook Scar Assessment Scale, al of which have the potential to contribute to better evaluation of scar properties in both research and clinical diagnosis.

Objective To analyse the change of the distribution ratio or diagnostic and therapeutic interventional procedures for cardiac patients at different stages during the past 15 years. Methods According to the date of admission the patients were divided into 2 groups:Group A (from 1987 to 1994,425 cases) and group B (from 1995 to 2001,1 623 cases).The constituent ratio of case with coronary heart diseases,arrhythmias,congenital heart diseases and other cardiovascular diseases,as well as the distribution ratio of various kinds of interventional procedures undergone were calculated,and the date of the two stages were compared.Results The mean catheterizations per year of Group A and Group B were 53?18 and 232?156 respectively ( P

0.05). PetCO 2 was a reliable index reflecting PaCO 2. There was a significant difference between PetCO 2 and PaCO 2 in the Group Ⅲ (P

Cooperation networks of nursing techniques were constructed by way of perspective practice so as toenhance the quality of nursing critical cases, the success rate of emergency treatments and patients' life quality. Themeasures included taming in techniques for nursing critical cases, making the runds of wards and consultations, andenlarging the scope of the cooperation networks. Quantification methods were introduced into management, networks ofcritical case assessment were ed in managing patients, and the system for assessing taming in techniques for nursingcritical cases was used in managing nineing personnel, thus improving the quality of the nursing personnel, ensuringthe rational allocation of human resources and promoting the enhancement of the hospital's medical quality and its social and econondc benefits. It is therefore not difficult to see that the cooperation networks are of application and dissemination value.

Objective To explore the risk factors for acute kidney injury (AKI)in patients with acute myocardial infarction (AMI).Method The medical data of hospitalized patients with AMI admitted from October 2013 to May 2014 were reviewed.All patients were divided into AKI group and non-AKI group.The univariate comparison analysis were performed to obtain the AKI risk factors.Results A total of 565 patients were enrolled.The incidence of AKI (n =91 )was 16.1% and there were 474 non-AKI patients.The mortality of AKI group was 19.8% and mortality of non-AKI group was 0.4% (P <0.01). Univariate analysis demonstrated that the risk factors of AKI were age,hypertension,previous myocardial infarction,heart failure history,chronic kidney disease,cerebral infarction history,peripheral vascular disease;ventricular fibrillation,heart rate,Killip grade ≥3 stage,left ventricular ejection fraction,serum creatinine,eGFR,hemoglobin,blood urea nitrogen,troponin I,B-type natriuretic peptide and C-reactive protein,fasting glucose,albumin,maximum daily dose of furosemide,non-use of ACEI /ARB and statins, the use of intra-aortic balloon pump, temporary pacemaker and pulmonary mechanical ventilation, implementation of PCI and coronary artery bypass graft surgery.Conclusions These risk factors for AKI after AMI were found to identify high-risk patients,helping the clinicians to make decision for preventive intervention.

Objective To investigate the pattern of plasma kisspeptin levels in normal female during various pubertal Tanner stages and the girls with idiopathic central precocious puberty(ICPP) or with premature thelarche(PT), and to evaluate the significance of detecting plasma kisspeptin levels as a new criterion for early differentiation between ICPP and PT.Methods Each study group of normal pubertal females with Tanner stage Ⅰ to Ⅴ comprised 16 to 19 individuals.The levels of plasma kisspeptin were also detected in girls with ICPP(n= 10)or PT(n = 12).The plasma kisspeptin levels were detected by enzyme-linked immunosorbent assay (ELISA).Results The level of kisspeptin was significantly higher in ICPP group than in that of PT group [(1.73±0.23 vs1.43±0.29) ng/ml, P＜0.05].Among the normal pubertal females, the level of kisspeptin decreased gradually from Tanner stage Ⅱ to Tanner stage Ⅴ, being highest in Tanner stage Ⅱ [(1.73±0.22) ag/ml] ,lower in stage Ⅳ and Ⅴ than in stage Ⅰ and Ⅲ (P＜0.01).Conclusions Plasma kisspeptin level was the highest during Tanner stage Ⅱ in normal female pubertal development.It is significant to detect plasma kisspeptin level for the differential diagnosis of ICPP and PT.