Objective To investigate a simple, valuable new method for coverage of the soft tissue defect over the lower one-third of the leg, ankle and the foot. Methods The distally based peroneus brevis muscle flaps were applied for coverage of the soft tissue defects over the lower one-third of the leg, the ankle and the foot. The muscle flaps were covered with split-thickness skin grafts. Results The distally based peroneus brevis muscle flaps were applied for coverage of the soft tissue defects over the lower one-third of the leg, the ankle and the foot in 16 cases. The largest area of the soft tissue defect was 5 cm?7 cm. The smallest was 3 cm?4 cm. Primary healing occured in 14 cases undergoing muscle flap construction, second-stage healing occured in 2 cases, no total flap necrosis occured in any cases. Conclusion This technigue is a simple and complications are lesser. The successful rates are higher. This muscle flap is suitable to the mudium or small soft tissue defect especially.

Aim To invesitgate the effect of PTEN(phosphatase and tensin homologue deleted on chromo-some ten) /PI3K(phosphatidylinositol 3 kinase) signaltransduction pathway in hypertrophied myocardium me-diated by overloaded pressure in rats,and the effect ofangiotensionⅡ(AngⅡ) receptors(AT1,AT2) antag-onists on it.Methods Overloaded pressure hypertro-phied myocardiumratmodel was established by abdom-inal arota constriction.Thirty two male wistar rats weredivided randomly into four groups,namely sham-opera-ted group,banding group,valsartan group(bandinggroup and valsartan administation),and PD123319group(banding group and PD123319 administration).AngⅡconcentration in plasma and left ventricular myo-cardium were measured by radioimmunoassays.Cardiacindex was measured and HE staining was performedwith left ventricular myocardium,immunoprecitipationwas used to assay the protein expression and phospho-rylation of PTEN、PI3K and Akt(Protein kinase B),protein expression of?-skeletal-actin in myocardial tis-sues.Results AngⅡ concentration in serum and leftventricular myocardium tissue in banding group washigher than that in sham-operated group,valsartangroup andPD123319 group(P0.05).Conclusions PTEN/PI3Ksignal pathway is involved in myocardium hyper-trophy in overloaded pressure rat model.The up-regu-lations of PI3K/Akt and down regulation of PTEN aremediated via AT1 and can be reduced by AT1 receptorantagonists.

AIM: To establish a method of analysing chemical components of the essential oil in Mulberry Leaves in Xinjiang. METHODS: The essential oil of Mulberry Leaves was extracted by ultrasound-steam distillation method.components were measured by GC-MS.About 80 peaks were separated totally.55 components of which were identified.The amount of the components from the essential oil was determined by normalization method.(RESULTS:) There were alkane(C_(10)～C_(40)),alkene,alcohols,unsaturated aldehydes,unsaturated ketones,carboxylic acid,ester,heterocyclic compound and aromatic hydrocarbon in essential oil in Mulberry Leaves.The main components were octadecane(9.11%),1,2-benzenedicarboxylic acid-bis(2-methyl)propyl ester(8.92%),3,7,11,15-tetramethyl-2-hexadecen-1-o1(7.19%).there was a small amount of terpene in essential oil in Mulberry Leaves. CONCLUSION: The method is reliable,stable and has a good repeatability.This method can be applied to the analysis of the essential oil components extracted from Chinese traditional medicine.

Objective:To explore the effect of family integrated care (FIC) combined with intelligent health education platform in health education for elderly patients with stroke.Methods:Eighty patients with stroke were included from the Department of Neurology of the Second Affiliated Hospital of Chongqing Medical University from June 2019 to May 2020. They were divided into the experimental group and the control group ( n=40, respectively) by the random number table method. Patients in the control group received routine FIC in the Department of Neurology. Additional intelligent health education platform was provided for the experimental group to carry out intensive education. The modified Barthel index scale, self-care compliance scale and hospital anxiety and depression scale (HADS) were used before and after the intervention to evaluate patient′s self-care ability, self-care compliance and the degree of anxiety and depression. Results:Finally, 39 patients in the experimental group (1 case lost to followed-up) and 37 patients in the control group (3 cases lost to followed-up) completed the study. After the intervention, the scores of the modified Barthel index and self-care compliance scale of the experimental group were significantly higher than those of the control group [(65.9±12.6) vs (60.1±11.9) points, (78.2±13.6) vs (71.4±14.6) points], the HADS score was significantly lower than the control group [(15.18±2.46) vs (19.46±2.40) points] (all P<0.05). The scores of the modified Barthel index scale and self-care compliance scale of the experimental group after the intervention were significantly higher than those before the intervention [(65.9±12.6) vs (40.8±12.5) points, (78.2±13.6) vs (54.6±15.9) points] (all P<0.05), the HADS score was significantly lower than before the intervention [(15.18±2.46) vs (21.74±3.52) points] (all P<0.05). Similarly,the scores of the modified Barthel index and self-care compliance scale of the control group after the intervention were significantly higher than before the intervention [(60.1±11.9) vs (41.6±9.72) points, (71.4±14.6) vs (54.3±14.8) points], the HADS score was significantly lower than before the intervention [(19.46±2.40) vs (21.38±3.09) points] (all P<0.05). Conclusion:FIC combined with intelligent health education platform can significantly improve the self-care ability and self-nursing compliance of elderly stroke patients, and markedly improve the degree of depression and anxiety.

Mammalian carboxylesterases hydrolyze a wide range of xenobiotic and endogenous compounds, including lipid esters. Physiological functions of carboxylesterases in lipid metabolism and energy homeostasis in vivo have been demonstrated by genetic manipulations and chemical inhibition in mice, and in vitro through (over)expression, knockdown of expression, and chemical inhibition in a variety of cells. Recent research advances have revealed the relevance of carboxylesterases to metabolic diseases such as obesity and fatty liver disease, suggesting these enzymes might be potential targets for treatment of metabolic disorders. In order to translate pre-clinical studies in cellular and mouse models to humans, differences and similarities of carboxylesterases between mice and human need to be elucidated. This review presents and discusses the research progress in structure and function of mouse and human carboxylesterases, and the role of these enzymes in lipid metabolism and metabolic disorders.
Amino Acid Sequence ; Animals ; Carboxylic Ester Hydrolases ; chemistry ; genetics ; metabolism ; Humans ; Intracellular Space ; metabolism ; Lipid Metabolism ; Mice ; Polymorphism, Single Nucleotide ; Protein Domains