Objective To study the effect of licorice on the pharmacokinetics of lovastatin in hyperlipidemic rat model.Methods Eighteen rats were randomly divided into control group (n =6) and test group (n =12).Rats in the test group were administered high fat diet to construct hyperlipidemic rat model.The 12 hyperlipidemic rats were then randomly divided into two groups:lovastatin group (n =6) and lovastatin combined with hcorice group (n =6).The rats in both groups were administered lovastatin capsule (20 mg/kg,0.5％ CMC-Na solution) after receiving licorice (for lovastatin combined with licorice group) or saline (for lovastatin group) for 7 days.Blood samples were collected at different time points before and after the administration of lovastatin capsule.The plasma concentrations of lovastatin and lovastatin acid (an active metabolite of lovastatin) were determined by LC-MS/MS method.Pharmacokinetics parameters were calculated using DAS 2.0 software,and the two groups were compared using SPSS 18.0 software.Results Long-term administration of licorice resulted in a significant increase in the plasma level of lovastatin acid in the hyperlipidemic rat,and the corresponding mean Cmax was approximately 80％ higher than that of the lovastatin group (P ＜ 0.05),while AUC0-t and AUC0-t increased by 115％ and 109％,respectively (P =0.005 and P =0.027).Cmax and AUC of lovastatin also increased,but there was no statistical significance (P ＞ 0.05).Conclusion Licorice can inhibit the metabolism of lovastatin in hyperhpidemic rats and increase its exposure in vivo.

Objective To explore the change of the activities of peroxisome ?-oxidation in T2DM patients with the lipid disorders.Methods We analyzed the changes of the activities of peroxisome ?-oxidation and fatty acyl-CoA oxidase and its mRNA expression in 112 cases of T2DM patients.Results We found that there was compensatory increase in activity of peroxisome ?-oxidation of T2DM patients.As compared with control group,the activity of fatty acyl-CoA oxidase of T2DM group was increased by 5%(P

Sepsis causes marked derangements in the flow of glutamine among organs.During sepsis,skeletal muscle exhibits increase in glutamine release which is associated with a significant increase in endogenous glutamine biosynthesis.However, the release rates exceed that of synthesis.The liver appears to be the major organ of glutamine uptake,the bowel displays a decrease in glutamine utilization and the cells of the immune system are also major glutamine consumers.

Objective To evaluate short-term curative effect of chloroquine combined with traditional Chinese medicine in treatment of erosion lichen planus. Methods 40 cases were chosen and randomly divided into two groups. The treatment group included 20 cases were treated with chloroquine combined with traditional Chinese medicine. The control group with 20 cases was treated only with chloroquine. The curative effect of the two groups were compared. Results Comparing the two groups, in the treatment group, the pain was remarkably relieved and the erosion areas were obviously smaller. Significant changes were observed statisticly. The overall effectiveness of the treatment group was 85% ,but that of the control group was 65%. Conclusion The treatment of chloroquine combined with traditional Chinese medicine have a short-term clinical effect on erosion lichen planus.

Objective To investigate the method for benefit management of medical equipment. Methods By analyzing the actuality of equipment management, problems were founded and the solutions were discussed. Results The problems are the repeated purchase of medical equipment, less use in some equipments, dis- accordance among equipment, card and account. Conclusions In hospital, medical equipment play a crucial role in medical affairs, teaching and scientific research, and can bring prominent economic benefit. Enhancing the scientific management in medical equipment, emphasizing the details, procedure and scientific investigation in management can promote the using efficiency, ensure the reasonable match in equipment as much as possible in order to make the hospital sustainable development.

Objective To investigate the effect of warfarin on the formation of renal calcium oxalate stones induced by 1% ethylence glycol (EG) and 3% ammonium chloride (NH 4Cl) in rats. Methods 30 adult male Wistar rats were randomized to three groups,the control group (A) being non-treated,the stone group (B) rats taken EG and NH 4Cl,and the warfarin group (C) taken warfarin in addition to EG and NH 4Cl.All rats were bred 4 weeks.The renal tissue calcium,the renal calcium oxalate crystallization,and the urinary calcium excretion and serum biochemical data were detected. Results No significant difference has been noted in 3 groups,although there is a tend that number of ranal calcium oxalate crystals in group C was more than that in group B. Conclusions The results denoted that warfarin had no significant effect on the calcium oxalate crystallization in rats.

Risk of radiation limits the use of radioactive isotopes in humans and the use the stable isotopes increases. The improvement of the mass spectrometers has resulted in the development of a variety of approaches for measuring rates of protein synthesis.Currently, two methods (constant infusion method and flooding method)have been extensively used.

Objective To improve standards of diagnosis and therapy for acute focal bacterial nephritis bY comparing the characters of acute focal bacterial nephritis and acute pylonephritis.Methods Thirty-five patients of upper urinary tract infection whoever accepted ultrasonographic and computed tomographic (CT) examinations in Beijing Hospital from January 2007 to January 2013 were studied retrospectively.Eighteen patients were diagnosed as acute focal bacterial nephritis (AFBN) according to CT imaging features,the other 17 patients were diagnosed as acute pyelonephritis (non-AFBN).The clinical feature,laboratory and imaging examination were compared between the two groups.Results Leukocyte count,percentage of neutrophil,fasting blood-glucose,ESR,NAG/Cre of urine,the combination of more than one kind of antibiotics and duration of antibiotic treatment were higher or much more in AFBN group patients than non AFBN patients,the differences between two groups were statistical significance(P ＜ 0.05).Urine microorganism culture was mainly Escherichia coli no matter in AFBN group or non-AFBN group,microorganism species of AFBN patients were more complex than non-AFBN patients.The most common contrast-enhanced CT feature of AFBN included local distribution of inflammatory area,wedge-shaped or unregular-shaped hypodense lesions on bilateral or single kidney,and mass-like hypodense lesions in more severe cases.NAG/CRE of urine was higher in AFBN group patients with bilateral kidney lesions than with single kidney lesions and non AFBN patients.The remission time of AFBN and non-AFBN was (3.4 ± 2.9) d,(3.0 ± 1.8)d respectively.The relapsing rate of AFBN and non-AFBN within half a year was 2/18,2/17,respectively.Conclusions The symptoms of acute focal bacterial nephritis are not specific.Abdominal contrast-Enhanced CT,ESR,leukocyte count,urine NAG/CRE are probably a predictor of AFBN.Antibiotic treatment with appropriate usage and sufficient duration could bring satisfactory outcomes and prognosis in acute focal bacterial nephritis and pyelonephritis patients.

ObjectiveTo investigate the effects of comprehensive geriatric assessment (CGA) intervention on depression and anxiety in patients with benign prostatic hyperplasia (BPH).Methods90 patients with BPH (aged 65 years and over) from June 2009 to June 2010 were randomly assigned to control(n= 45) and CGA (n = 45) group.Control group received conventional therapy,while CGA group was given CGA and consultation therapy as well as conventional therapy.The scores of international were assayed and compared between two groups before and 6 months after treatment.ResultsThe scores of international prostate symptom score (IPSS),quality of life scale (QOLS),self-rating depression scale (SDS) and self-rating anxiety scale (SAS) in control (t= 11.0,5.84,7.08and 9.68,respectively) and CGA (t= 14.0,10.4,9.16 and 6.1,respectively)group 6 months after treatment reduced as compared with those before treatmen (all P ＜ 0.01).After 6 months of treatment,the scores of IPSS,QOLS,SDS and SAS in CGA group were lower than in control group (t= 4.25,5.55,3.45 and 2.88,respectively,all P＜0.01).ConclusionsCGA intervention can improve the quality of life in BPH patients and alleviate their depression and anxiety.

Objectives To investigate the distribution of uridine diphosphate-glucuronosyltransferase 1A1 (UGT1A1) gene promoter polymorphism and its relation to the toxicities caused by irinotecan in Chinese patients with cervical cancer and ovarian cancer.Methods Sixty-four blood samples were taken from the patients with ovarian cancer and cervical cancer.The DNA was extracted and amplified with PCR.Then,the sequences of UGT1A1 gene promoter were detected by capillary electrophoresis allele fragment analysis (size-based analysis) methods.The relationship between UGT1A1 gene promoter polymorphism and the toxicity caused by irinotecan was analyzed.Results In all the patients,TA 6/6 was the most common genotype of UGT1A1 gene promoter (44 cases),accounting for 69％ (44/64),followed by genotype of TA 6/7 ( 17 cases,27％,17/64),while genotype TA 7/7 was rare (3 cases,5％,3/64).The genotypes of UGT1A1 gene promoter was an independent factor for the occurrence of delayed diarrhea (P =0.040,OR =4.228,95％ CI:1.065 - 16.785 ) but not for neutropenia ( P =0.068,OR =3.659,95％ CI:0.911 - 14.700).The patients with both genotype TA 6/7 and TA 7/7 presented much higher risk of delayed diarrhea and neutropenia than those with TA 6/6 ( all P =0.001 ).Conclusions UGT1 A1 gene promoter polymorphism may be a significant influencing factor for delayed diarrhea.The patients with both genotype TA 6/7 and TA 7/7 could present much higher risk for delayed diarrhea than those with TA 6/6,while genotype TA 6/6 may be the most common UGT1A1 promoter type in Chinese patients with cervical or ovarian cancer.