Objective To evaluate the clinical effect of straight incision in the treatment of scapular neck and body fractures .Methods 62 cases with scapular neck and body fractures were randomly divided into the obser -vation group (30 cases) and control group (32 cases).The control group was given the traditional Judet approach operation,the observation group was given the straight incision operation .The general situation of operation ,fracture healing time ,clinical efficacy and postoperative complications were observed .The improvement of joint function after operation was evaluated .Results The operation incision ,operation time and intraoperative blood loss of the observa-tion group were significantly better than those of the control group (t=9.599,9.506,21.297,P<0.01).The fracture healing time between two groups had no statistically significant difference (t=0.922,P=0.35).The effective rate of fracture healing in the observation group was 93.3%,which was significantly higher than 71.9%of the control group (χ2 =4.885,P<0.05).The incidence rate of complication in observation group was 13.3%,which was lower than 37.5%in the control group(χ2 =4.723,P<0.05).The score of daily activities in observation group was better than the control group(t=3.761,P<0.01).Conclusion The straight incision operation is more minimally invasive in the treatment of scapular neck and body fractures compared with Judet approach ,and it has better prognosis .

Purpose To study the expression of zeb-1 and c-jun in gastric cancer and its influence of occurrence and development of tumor. Methods The expression zeb-1, c-jun and E-cadherin were assessed in 100 specimens of gastric cancer and their tissue adja-cent to cancer by immunohistochemistry. Results The positive rates of zeb-1,c-jun and E-cadherin expression in gastric cancer were 81%, 70%, 35%, and in tissue adjacent to cancer were 17%, 25%, 100%. The expression of zeb-1 and c-jun in gastric cancer were higher than those in their tissue adjacent to cancer (P<0. 05). zeb-1 expression was closely correlated with the degree of tumor differentiation, depth of invasion, lymph node metastasis and TNM stage (P<0. 05), which were not correlated with patient s age, sex and tumor size. c-jun expression was closely correlated with the degree of tumor differentiation (P<0. 05), which were not correlated with other pathological features. The expression of zeb-1 was negatively correlated with the expression of E-cadherin and was positively correlated with the expression of c-jun in gastric cancer. Patients with high expression of zeb-1 and c-jun had lower five-year survival rate than patients with negative expression. Conclusion zeb-1 and c-jun are closely correlated with occurrence and development of gastric cancer. They can be a index of judging prognosis of gastric cancer.

BACKGROUND: Ovariectomized animals usually are employed for the study of women osteoporosis while little is known about theenergy balance of ovariectomized animals. Many previous studies showed that soy isoflavone could decrease the hyperlipemia resulted by high-fat feed, and how about the effect of soy isoflavone on the energy metabolism in ovariectomized animals?OBJECTIVE: To explore the pathologic effect of soy extract and its active components on the energy metabolism in ovariectomized rats so as to provide adequate evidences for the primary rehabilitation and prevention of type Ⅱ diabetes mellitus, disturbance of lipid metabolism as well as hypertension and coronary atherosclerotic heart disease.DESIGN: A randomized and controlled trial with the experimental animals as subjects.SETTING: Laboratory of Cell and Biochemistry in a university.MATERIALS: Ninety Wistar rats (SFP grade, license code: scxk11-00-006) were randomized into 9 groups: normal group, sham group,model group, estrogen group, high-dose soy flavone group, low-dose soy flavone group, high-dose soy extract group, low-dose soy extract group and soy polysaccharide group, with 10 rats in each group.INTERVENTION: Except the rats in normal group and sham group,the bilateral ovaries of other rats were all removed. From one week after operation, body weight and daily food intake were detected once a week.Six weeks later, the rats were killed to calculate the forage transformation efficiency, measure body length, work out the body mass index (BMI),and separate the abdominal fat and weight.MAIN OUTCOME MEASURES: ①Effect of soy extract on the abdominal fat accumulation of ovariectomized rats. ②Effect of soy extract on food intake of ovariectomized rats. ③Effect of soy extract on forage transformation efficiency of ovariectomized rats.RESULTS: After ovariectomy, the food intake, body weight and BMI all raised, and the forage transformation efficiency increased, with abdominal fat accumulation. The changes of energy metabolism induced by ovariectomy were all weakened at different degrees in estrogen group, soy extract group and soy flavone group, while no influenced was found in soy polysaccharide group.CONCLUSION: The ovariectomized rats can be used as animal model of the climacteric fat women. The soy extract, with the effective component of flavonoid, can reduce the pathologic changes such as the increase of food intake, body weight and forage transformation efficiency induced by ovariectomy.

Objective To investigate the effect of dexmedetomidine (Dex)on long-term cogni-tive function after partial hepatectomy (PH)in mice.Methods Forty adult male C57BL/6J mice were randomly divided into control group (group C),group PH,normal saline group (group NS)and group Dex,10 mice per each group.At 2 days after PH,the mice of group C and group PH under-went Morris water maze test,four times per day and a total of 4 days.The mice were euthanized and hippocampi tissue was immediately excised for determination of wet hippocampi weight to dry hipp-ocampi weight (W/D)and total hippocampi water content (TWC).The expression levels of cycteinyl aspirate-specific protease 12 (caspase-12)mRNA and protein of hippocampi tissue were detected re-spectively by reverse transcription-polymerase chain reaction (RT-PCR)and Western blot.Apoptosis index (AI)of hippocampi tissue was determined by terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL)assay.After the mice regained consciousness after operation,animals re-ceived intraperitoneally Dex at a dose of 25 μg·kg-1 ·d-1 for 30 consecutive days in group Dex.Ani-mals of group NS were given intraperitoneally with the same volume of normal saline.After treatment for 30 consecutive days,the mice of group Dex and group NS underwent Morris water maze test,four times per day and a total of 4 days.The mice were euthanized and hippocampi tissue was immediately excised for determination of W/D and TWC.The expression levels of caspase-12 mRNA and protein of hippocampi tissue were detected respectively by RT-PCR and Western blot.AI of hippocampi tissue was determined by TUNEL assay.Results Compared with the first day,the escape latency at the third day,the fourth day and swimming distance were shortened in groups C,NS and Dex (P <0.05);Compared with group C,the escape latency at the third day,the fourth day and swimming distance were prolonged (P < 0.05 ), W/D,TWC and AI were higher (P < 0.05 ), and the expression levels of caspase-12 mRNA and protein of hippocampi tissue were significantly higher (P<0.05)in group PH.Compared with group NS,the escape latency at the third day,the fourth day and swimming distance were shortened (P <0.05),W/D,TWC and AI were lower (P <0.05)and the expression levels of caspase-12 mRNA and protein of hippocampi tissue were significantly lower (P <0.05)in group Dex.Conclusion The treatment of Dex could improve the long-term cognitive function after PH in mice,and the mechanism may be to lighten apoptosis mediated by caspase-12 in hippocampi tissue.

This paper explored effect of AF internal fixation system in reduction of lumbar vertebral fracture.AF internal fixation system acts fixation and reduction roles for most of spinal blowout fracture through spinal hyperextension or devise opening to reduce fracture blocks in vertebral canal or anterior vertebrae.In addition,it alleviates neural compression to some extent.The collapsed vertebral reduction is achieved by traction of anterior and posterior longitudinal ligaments as well as intervertebral annulus fibrosus;the physiologic curvature and vertebral height are restored.Moreover,the spinal stability is maintained.Early functional exercise can promote fracture healing and reduce complications.In treatment of lumbar vertebral fracture by AF internal fixation system,screw entry,reduction,traction,and fusion are completed based on host biomechanics,and biocompatibility of implant and host should be paid more attention.

Objective To explore the possible functional mechanism of Er-xian Decoction and its component herbs on the expression of extrogen receptor(ER) in adrenal development rats.Methods Fifty-four development female SD rats were randomly divided into nine groups:normal control group(A),positive control group(B),Er-xian Decoction group(C),Herba Epimedii group(D),Rhizoma Curculiginis group(E),Radix Morindae Officinalis group(F),Radix Angelicae Sinensis group(G),Cortex Phellodendri group(H),Rhizoma Anemarrhenae group(I).After the rats were administrated for six days,serum were got to measure testosterone(T).Bilateral adrenal gland was weighed and embedded by paraffin.Mean optical density(MOD) were studied by the immunohistochemical method and image analyzing system.Results The adrenal coefficient was increased in group B and C,and the level of T was decreased in group C,D,E and I.ER? was mainly observed in the cytoplasm of zona glomerulosa and zona fasciculate.MOD in group C was increased while declined in group B.ER? was mainly observed in nucleus of zona glomerulosa and zona fasciculate.MOD in group C,D,E and I was less than group B.Conclusion Er-xian Decoction and its component herbs of warming Shen(Herba Epimedii,Rhizoma Curculiginis) and nourishing Yin(Rhizoma Anemarrhenae) play a phytoestrogen role by influencing the expression of ER? and ER ? in the adrenal cortex.

Objective To investigate the effect of puerarin on proliferation of human embro fibroblast and extracelluar matrix. Method Human embro fibroblasts were incubated with 0～400 ?g/mL puerarin for 24～96 h. The MTT method was used to assay the biological activities and extracelluar matrix formation in different time and different dose of pueratin. The cell cycle distribution was detected by flow cytometric analysis. Result Incubation cells in presence of puerarin of 40～400 ?g/mL for 24～72 h could significant inhibit cell proliferation and restrain the collagen synthesis in a dose- and time-dependent manner (P

Objective To observe the therapeutic effects of curcumin on pulmonar y fibrosis rats induced by bleomycin. Method One hundred and forty-four male Sp rague-Dawley rats were randomly divided into 6 groups (24 rats in each group). Rats in the model control group,positive medicine group,and high-,moderate-and low-dosage curcumin groups were injected with a single dose of bleomycin th rough trachea,and rats in sham-model control group with the same volume of nor mal saline. One day after the injection,curcumin solution of different dosages (200,100,50 mg?kg-1?d-1) was respectively given to rats in the high-,mod erate-and low-dosage curcumin groups daily by gastric gavage,while the same v olume of normal saline was given to those in the sham-model control group and m odel control group,and the same volume of prednisone(0.56 mg?kg-1?d-1) was given to those in the positive medicine control group. On the 7th,14th,and 28t h day after medication,8 rats in per treatment group were randomly killed,the changes of lung function were observed,and the lung was incised to make patholo gical sections which were stained with HE and Mallory. Results Curcumin improved lung function. The results of pathological examination showed that curcumin rel ieved the pathogenic changes of the lungs of pulmonary fibrosis rats induced by bleomycin. Conclusion Curcumin exerts a certain curative effect on pulmonary fib rosis rats induced by bleomycin.

Aim To investigate the phytoestrogenic effects and mechanism on ER?,ER? expression levels at the protein of quercetin and psoralen in T47D cells.Methods T47D cells were treated with 10 ?mol?L-1 quercetin and 10 ?mol?L-1 psoralen for 48h respectively,total cell extracts examined for the levels of ER? and ER? protein by RT-PCR and Western blot analysis.The estrogen-like effect of quercetin and psoralen and their relations with the estrogen-receptor were evaluated by pure estrogen receptor antagonist ICI182,780 as a tool.Results Psoralen(10 ?mol?L-1) and quercetin(10 ?mol?L-1) up-regulated the expression of ER? mRNA and protein,without any effect on ER? mRNA and protein levels.The above up-regulation on ER? protein could be inhibited by adding estrogen receptor antagonism ICI182,780.Conclusion Psoralen and quercetin exerts estrogenic activity to ER-positive cells proliferation through interaction with ER? expression.

objective To evaluate the value of HRCT on differential diagnosis of a rat model of pulmonary fibrosis induced with bleomycin A5 and to replicate a rat model of pulmonary fibrosis for radio imaging study to investigate pathogenetic mechanism of pulmonary fibrosis. Methods In 50 of 60 male Sprague Dawley rats bleomycin A5 was given through the trachea, and the other 10 received same volume of normal saline. After 28 days, HRCT and pathological examination of the lungs were performed, and the changes were correlatively studied. Results HRCT showed pulmonary fibrosis in all the 50 rats treated with bleomyein, including ground glass opacity, nodular shadow, abnormality in bronchio vasarlar linings, pleural thickening, and in some with beehive like changes. Lung fibrosis was corroborated by pathological sections. Conclusion HRCT can be used to diagnose pulmonary fibrosis of living rats. It is a simple, accurate, reliable, and it can give good representation.