1.The relationship between ECochG and glycerol test in vertigo patients (Report of 112 cases)
Jiezhen LU ; Jianguo ZHANG ; He LAI
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2000;(11):510-511
Objective:For further investigating the diagnostic value of the ECochG in Meniere's disease.Method:112 vertigo patients including Meniere's disease, sudden deafness, cervical vertigo and other vertigo dis-ease, had been done ECochG,glycerol test,audiometry and caloric test. The results were compared with those ofnormal group. Result:In Meniere's disease,the positive rate of abnormal-SP/AP in ECochG was 54.69% andthe positive rate of glycerol test was 53.12%. There are no statistics difference ( P >0.05) between this twotests with X analysis. Conclusion :We think that the glycerol test and other relative examination are necessary forthe diagnosis of Meniere's disease while the-SP/AP >0.4 ms in the examination of ECochG.
2.In vitro study on influence of residual methylene blue after virus inactivation plasma on immune function of human PBMC cells
Zhizhong CHEN ; Jiemin LI ; Shangliang CHEN ; Jiezhen LIANG ; Shaofen LU ; Chaohong CHEN ; Qianwen LU ; Qi ZHANG
International Journal of Laboratory Medicine 2016;37(16):2205-2207
Objective To study the influence of residual methylene blue after plasma viral inactivation on the human immune cell function by using the peripheral blood mononuclear cell(PBMC) .Methods PBMC were isolated by adopting the Ficoll‐Hypaque density gradient centrifugation method and co‐cultured for 72 h in presence of specific T cell stimulating factors(Anti‐CD3/28 and Anti‐CD28) ,with or without different concentration of methylene blue .The culture supernatant was collected and detected the cyto‐kines secretion situation by ELISA .After 66 h culture ,CCK‐8 dye was added and continueously cultured for 4-6 h ,the prolifera‐tion was determined at A450 .Results The high‐concentration doses of methylene blue (1 .25 ,2 .5 ,5 μmol/L groups) had signifi‐cantly inhibiting effect on the proliferation of PBMC stimulated by Anti‐CD3/28(P< 0 .01) ,its OD value was decreased from 0 .897 ± 0 .385 to 0 .632 ± 0 .334 ,0 .524 ± 0 .254 and 0 .445 ± 0 .287 respectively ,showing certain dose dependent effect .The high concentrations of methylene blue (1 .25 ,2 .5 ,5 μmol/L groups) could down‐regulate interleukin(IL)‐17a ,IL‐10 and interferon (IFN)‐γ secreted by anti‐CD28 induced PBMC ,moreover showing a dose dependent effect .1 .25 ,2 .5 ,5 μmol/L methylene blue af‐fected the IL‐17a level secreted by PBMC from (406 ± 57)pg/mL descending to (276 ± 38) ,(192 ± 31) ,(134 ± 24)pg/mL respec‐tively ;affected PBMC to secrete IL‐10 ,its level was reduced from (184 ± 15) pg/mL to (132 ± 13) ,(110 ± 12) ,(42 ± 8)pg/mL ;af‐fected PBMC to secrete IFN‐γ,its level was deduced from (4 512 ± 187)pg/mL to (2 876 ± 143) ,(2 234 ± 153) ,(1 988 ± 112)pg/mL respectively .Conclusion High concentrations of methylene blue (≥1 .25 μmol/L ) has the significant inhibiting effect on the proliferation and cytokine secretion functions of PBMC .In other words ,the residual methylene blue concentration in viral inactiva‐tion plasma (≤0 .33 μmol/L) has no obvious effect on the immune function of PBMC ,but whether this concentration of methylene blue having the effect on human pure T cell immune function needs to be further evaluated and studied .