Objective To investigate the clinical application of plain film without tension hernia repair combined with traditional Chinese medicine in the treatment of middle-aged and elderly inguinal hernia.Methods 60 cases of elderly patients with inguinal hernia from May 2014 to July 2016 in our hospital were selected and randomly divided into control group and research group,30 cases in each group.Control group was given plain film without tension hernia repair treatment,and research group was given Chinese medicine on the basis of oral treatment.Then clinical curative effect observation of two groups was observed and compared.Results After treatment,the team bowel sound recovery time,anus exhaust time,postoperative bed activity time and hospital stay of research group patients was significantly shorter than the control group,the difference was statistically significant(P<0.05),the total effective rate of the research group was as high as 96.7％,significantly higher than the control group 73.3％,the difference was statistically significant(P<0.05).The postoperative complications rate of control group was up to 36.7％,significantly higher than the study group 13.3％,the difference was statistically significant(P<0.05).Conclusion Clinical effect of plain elderly inguinal hernia without tension hernia repair was exact,and the clinical effect of oral medicine treatment can significantly shorten the length of hospital stay,lower incidence of postoperative complications.

The ORF sequence of glycosyltransferase gene BcUGT1 cloned from Bupleurum chinense DC. was analyzed and its three dimentional structure was predicted. Using qRT-PCR method, the expression characteristics of BcUGT1 after methyl jasmonate (MeJA) induction and in different plant tissues were investigated. The results showed that BcUGT1 may be involved in saikosaponin biosynthesis in B. chinense. Thereafter, the recombinant vectors of BcUGT1 were constructed for its expression in E. coli. The target protein was successfully expressed and purified. In the present study, three vectors, pRSET-A, pET-28a (+) and pET-30a (+), and three isolates of E. coli, BL21 (DE3) plysS, BL21A1 and BL21-CodonPlus (DE3)-RIPL were used under different induction conditions, such as different concentrations and during times of inducers (L-arabinose and IPTG) and different inducing temperatures. The results showed that in the condition of 0.5 or 1 mmol x L(-1) IPTG, 16 degrees C, 20 h, target protein expressed in BL21-CodonPlus (DE3)-RIPL with pET-28a (+) or pET-30a (+) as vector. Using PrepEase His-tagged protein purification kit, the target protein was purified. The present work will be helpful for follow-up bio-function analysis of BcUGT1.