Objective To establish a high performance liquid chromato-graphic method for determining the concentration of baicalin in healthy volunteers.Methods The assay was conducted on the Agilent HC-C18 (250 mm × 4.6 mm,5 μm)column with methanol-acetonitrile-phosphoric acid (47 ∶ 53∶0.2)as the mobile phase.The detection wave length was set at 280 nm,and wogonin was taken as the internal standard.Results The linearity range was 0.25～ 12.0 μg/ml with a regressive equation of Y=0.0094 C-0.00175 (r=0.9997).The recovery was above 80％,with both intra-day RSD and inter-day RSD below 10％.Conclusion The method was convenient,rapid,accurate,and suitable for the pharmacokinetic study of baicalin and monitoring its concentration of clinical application.