Objective To study the clinical value of HRCT in staging of lung interstitial disease(LID) in connective tissue disease(CTD).Methods 222 patients with CTD confirmed clinically underwent HRCT scan.The staging of LID according to the HRCT features of LID were done and the therapeutic effect was compared between each stage.Results In 222 cases ,64 cases were negative on HRCT as stage 0,158 cases had disseminated LID in different degree,including stage Ⅰ in 107,stage Ⅱ in 36 and stage Ⅲ in 15.There were significant statistically between the therapeutic effect and staging of LID.Conclusion The staging of CTD with LID by HRCT is helpful for judging the extent of LID and clinical treatment.

OBJECTIVETo develop a method for the simultaneous determination of aflatoxin B1, B2, G1, G2 and ochratoxin A in Glycyrrhiza uralensis by HPLC-FLD after immunoaffinity column with online post-column photochemical derivatization.

METHODSample was extracted with MeOH: H2O (80:20) and cleaned up by immunoaffinity column. The toxins were separated by reversed-phase HPLC and the mobile phase was consisted of methanol and 0.5% acetic acid solution with gradient elution. The determination was carried out by fluorescence detector after photochemical derivatization.

RESULTThe detection limits of aflatoxin G2, G1, B2, B1 and ochratoxin A were 0.02, 0.06, 0.015, 0.03 and 0.25 microg x kg(-1), respectively. The recoveries of analytes were from 76.0% to 103% and the relative standard deviations (RSDs) were below 13%.

CONCLUSIONThe method is a simple, accurate and can be used to determine the contents of aflatoxin B1, B2, G1, G2 and ochratoxin A in G. uralensis simultaneously.

Aflatoxins ; chemistry ; isolation & purification ; Chromatography, Affinity ; methods ; Chromatography, High Pressure Liquid ; methods ; Glycyrrhiza uralensis ; chemistry ; Ochratoxins ; chemistry ; isolation & purification ; Photochemical Processes

Radiotherapy is widely used in the treatment of primary and metastatic malignant tumors. It is traditionally believed that the killing effect of radiotherapy on tumor is based on the direct or indirect damage of ionizing radiation to DNA. In recent years, the anti-tumor role and mechanism of anti-tumor immune response induced by ionizing radiation have captivated widespread attention and achieved significant progress. Among them, Cyclic GMP-AMP synthase (cGAS)-stimulator of interference genes (STING) pathway is considered to be one of the key regulatory hubs. cGAS is a cytoplasmic DNA receptor that can bind to tumor-derived double-stranded DNA and activate the downstream STING, thereby activating anti-tumor immune response of the host. In view of the latest progress in this field, the important role and potential mechanism of cGAS-STING pathway in radiotherapy immune effect were mainly summarized, and the application prospect of targeting cGAS-STING pathway in radiotherapy sensitization was explored.

Objective To study the changes of blood coagulation function in early stage of acute renal failure patients, and illustrate its significance. Methods From October 2011 through January 2013, 80 patients with acute renal failure in the Nephrology Department and Pediatrics Department of Yuebei People’s Hospital were enrolled in this study,50 health people in the same period as control group,then compare the differences in creatinine, blood urea nitrogen, blood platelet(PLT), activated partial thromboplastin time(APTT), prothrombin time(PT), thrombin time(TT), plas-ma fibrinogen (FIB) between the two groups. Results The FIB levels and PLT count in observation group were higher than those of controls group (P<0.05), but APTT,PT,TT showed no statistically significant difference (P>0.05). Con-clusion Hypercoagulabale state could occur in patients with acute renal failure in early stage, abnormal clotting may be participates in occurrence of acute renal failure.