1.Relation of new-type drugs using intention to self-efficacy and perceived stress in secondary vocational school students
Jiayan CHEN ; Jincong YU ; Qingfeng WU ; Jiepin CAO ; Liankun LI ; Yuncui WANG ; Dong YAN ; Shiwei TENG ; Zengzhen WANG
Chinese Mental Health Journal 2017;31(6):461-465
Objective:To explore the relation of new-type drug using intention,self-efficacy,and perceived stress in secondary vocational school students.Methods:A total of 2692 secondary vocational school students in Zhaoqing,Guangdong Province were selected.The questionnaire of new-type drugs using intention,General Self-Efficacy Scale (GSES),and Perceived Stress Scale (PSS) were respectively used to assess the secondary vocational school students'new-type drugs using intention,self-efficacy,and perceived stress.Path analysis model was used to analyze the relationship of new-type drugs using intention,self-efficacy,and perceived stress among secondary vocational school students.Results:There were 13.6% (295/2165) of the secondary vocational school students reported an intention to use new-type drugs.The total scores of GSES and PSS were (2.4 ±0.5) and (18.8 ±5.0),respectively.The results of path analysis showed that,after controlling for age,gender,and residence,the scores of GSES were negatively correlated with the scores of PSS (γ =-0.21,P <0.001),and the scores of PSS were positively correlated with the scores of questionnaire of new-type drugs using intention (γ =0.05,P < 0.05).However,the scores of GSES showed no significant direct correlation with the scores of questionnaire of new-type drugs using intention (γ =0.04,P > 0.05).Conclusion:The secondary vocational school students' level of perceived stress are positively correlated with their intention to use new-type drugs.Self-efficacy might moderate the individual's level of perceived stress,thus to show indirect correlation to the secondary vocational school students' intention.
2. The relationship among depression, anxiety, stress and addictive substance use behavior in 5 935 secondary vocational students
Xiaodong YU ; Jincong YU ; Qingfeng WU ; Jiayan CHEN ; Yuncui WANG ; Dong YAN ; Shiwei TENG ; Yanting ZHAO ; Jiepin CAO ; Shuaiqi LI ; Yaqiong YAN ; Jie GONG ; Kang YAO ; Hui ZHOU ; Zengzhen WANG
Chinese Journal of Preventive Medicine 2017;51(3):226-231
Objective:
To investigate the relationship among depression, anxiety, stress and addictive substance use behavior in secondary vocational students.
Methods:
Cluster sampling method and the Adolescent Health-related Behaviors Questionnaire were used to collect demographic characteristics, psychological symptoms, and addictive substance usage among 5 935 students in nine vocational schools in Chongqing, Zhaoqing, Ningbo, and Taiyuan. Multivariate logistic regression analysis was used to analyze the relationship between the addictive substance use behavior and psychological factors.
Results:
The detection rates of depression, anxiety and stress were 46.5% (
3.Jianpi Yangzheng Xiaozheng Decoction Affect Proliferation and Stemness of Gastric Cancer HGC-27 Cells by Inhibiting Aerobic Glycolysis
Heyun TAO ; Yuanjie LIU ; Jiepin LI ; Shuhong ZENG ; Ying ZHANG ; Shenlin LIU ; Xi ZOU
Chinese Journal of Experimental Traditional Medical Formulae 2023;29(11):82-88
ObjectiveTo observe the effect of Jianpi Yangzheng Xiaozheng decoction (JYXD) on the proliferation and stemness of the human gastric cancer (GC) cell line HGC-27 by inhibiting aerobic glycolysis, and explore the underlying mechanism. MethodMethyl thiazolyl tetrazolium (MTT) assay was employed to determine the survival rate and chemotherapy sensitivity of HGC-27 cells treated with JYXD (0.25, 0.5, 1, 2, 4, 8, 16, 32 g·L-1). Colony formation assay was employed to detect the effect of JYXD (2, 4, 8 g·L-1) on the colony formation of the cells. The aerobic glycolysis level of HGC-27 cells after treatment with JYXD was measured by glucose assay kit and lactic acid assay kit. The proportion of stem cell subsets in HGC-27 cells was detected by flow cytometry. Western blot was employed to determine the expression of glycolysis-associated proteins such as lactate dehydrogenase (LDH), hexokinase 2 (HK2), glucose transporter 1 (GLUT1), and pyruvate kinase isozyme M2 (PKM2), and the expression of stemness-associated proteins such as octamer-binding transcription factor 4 (OCT4), SRY-box transcription factor 2 (SOX2), and Nanog. ResultJYXD (0.5, 1, 2, 4, 8, 16, 32 g·L-1) inhibited the activity of HGC-27 cells (P<0.05, P<0.01), with the inhibitory concentration 50(IC50) of 4.83 g·L-1, and it improved the sensitivity of HGC-27 cells to cisplatin chemotherapy. Compared with the control group, JYXD (2, 4, 8 g·L-1) reduced the colony formation number of HGC-27 cells (P<0.01) in a concentration-dependent manner. Flow cytometry showed that compared with that in the control group, the proportion of CD44+CD24+ALDH+ population in the cells treated with JYXD (2, 4, 8 g·L-1) decreased (P<0.05). In addition, JYXD (2, 4, 8 g·L-1) inhibited the glucose uptake and lactic acid production of HGC-27 cells. Western blot showed that compared with the control group, JYXD (2, 4, 8 g·L-1) down-regulated the expression levels of SOX2, Nanog, OCT4, PKM2, LDH, GLUT1, and HK2 (P<0.05, P<0.01) in a concentration-dependent manner. ConclusionJYXD may inhibit the proliferation and reduce the stemness of HGC-27 cells by regulating the aerobic glycolysis.