Background and purpose:Glutamine is the major fuel for the enterocyte and major energy origin of intestinal mucosal cell which prevent gastrointestinal mucosal cell from atrophying.Although there were a lot of studies of how to protect the gastrointestinal mucosal in various stress,such as trauma,burn and complex operation,but the impact of how the glutamine affected the gastrointestinal mucosa in the period of chemotheraphy was rarely reported.Our aim was to investigate the protective effects of glutamine for the morphological and functional damage of gastrointestinal mucosa induced by introperitoneally fluorouracil(5-FU) in rats in order to find the ways to prevent the intestinal mucosa from damage in the period of chemotherapy.Methods:Forty Wistar rats were randomly divided into four groups:A:Blank control group,B:5-FU group,C:5-FU + Glycin group,D:5-FU + Glutamine group.The rats in group C were given Glycin,group D glutamine(0.35 g/kg each).Group B,group C and group D received 5-FU(100 mg/kg) injection intraperitoneally on the fourth day respectively to make the model of gastrointestinal barrier injury.The content of endotoxin in peripheral blood was measured in all groups after the rats were killed on the seventh day.The mesenteric lymph nodes(MLN) and liver were harvested for comparison of bacterial translocation incidence among the four groups.Ileum tissue was taken for pathologic examination including comparison of mucosal thickness,villus height and width and morphology change of ileum of each group.Results:The test showed that the weight and the height,the width,the thickness of the villus intestinal mucosa of group D were more than those of groupB and group C.The content of endotoxin in peripheral blood and the positive cases of bacterial translocation in MLN and liver of group D were less than group B and group C.The weight and the height,the width and the thickness of the villus intestinal mucosa of group A were more than those of group B,group C,and group D.The content of endotoxin in peripheral blood and the positive cases of bacterial translocation of MLN and liver of group A were less than group B,group C and group D.Conclusion:There were damage to the rat's gastrointestinal mucosa during the giving of 5-FU intraperitoneally.Glutamine could protect rat's gastrointestinal mucosa from damage during the giving of 5-FU intraperitoneally.Glycin could not protect rat's gastrointestinal mucosa against from damage during the giving of 5-FU intraperitoneally.

objective To introduce the design concept and structure of a new type of lumbar intervertebral fusion cage ,and to e-valuate its biomechanical properties .Method A partially bioasorbable interbody fusion cage(PBIFC) made of nano hydroxyapatite and poly amino acid /calcium sulfate copolymer materials was developed .Range of motion(ROM ) ,compressive load ,and pull-out test on flexion ,extension ,lateral bending ,and torsion moment on fresh calf L3/L4 specimens of functional spinal union were carried out of iliac bone group ,PBIFC group ,and nano hydroxyapatite/polyamide 66(nHA/PA66) group .Result Of each movement ,the ROM value of iliac bone group are higher than the other two groups ,the difference was statistically significant (P<0 .05) ,and the ROM value of nHA/PA66 group are higher than PBIFC group ,but no statistical difference(P>0 .05) .The pull-out strength of PB-IFC group are higher than iliac bone group ,and the difference was statistically significant (P<0 .05);The pull-out strength of PB-IFC group is lower than the traditional group ,but no statistical difference(P>0 .05) .The compressive load of iliac bone group was lower than that of two cage group ,the difference was statistically significant (P<0 .05) .The compressive load of PBIFC group is slightly lower than the traditional group ,but no statistical difference(P>0 .05) .Conclusion With good implant stability ,pull-out resistance ,and compression resistance performance ,PBIFC can meet the biomechanics requirements of clinical implant .

Objective To investigate lymph node metastases in distal gastric cancer and its clinical significance. Methods From June 2006 to December 2007, 129 distal gastric cancer patients underwent radical gastrectomy with lymphadenectomy. Dissected lymph nodes were collected in groups, and histopathological studies were performed to detect lymph node metastasis. The relationship between lymph node metastasis and tumor parameters such as diameter, location, infiltrating depth, histological category,Borrmann typing was evaluated. Results Lymph node metastases was found in 80 out of 129 patients (62%). A total of 3295 lymph nodes were harvested with an average of 25.54 lymph nodes per patient,among those 889 lymph nodes were identified with metastasis. The metastasis rate was 18.60%, 48. 84%,37. 98%, 38. 76%, 44. 19%, 31.01%, 10. 85%, 14. 73%, 4. 65%, 1.55% and 0. 78% respectively in No. 1, No. 3, No. 4d, No. 5, No. 6, No. 7, No. 8a, No. 9, No. 11 p, No. 12a, No. 14v lymph node group.No. 3 and No. 6 group nodes were moat frequently invaded by metastasis. Conclusion This study provides the regular pattern of lymph node metastasis in distal gastric carcinoma patients undergoing radical gastrectomy and perigastric lymphadectomy which helps to guide lymphadectomy in terms of less trauma and favorite prognosis.

Objective To explore the correlation between the expression of serum long non-coding RNA bladder cancer associated transcript 1(LncRNA BLACAT1)and long non-coding RNA(LncRNA)-H19 levels and the severity of elderly knee osteoarthritis(KOA).Methods A total of 120 elderly KOA patients who underwent diagnosis and treatment in Jingmen Hospital of Traditional Chinese Medicine from November 2021 to February 2023 were selected as subjects(KOA group).Based on the Kelgren-Lawrence(K-L)classification,these KOA group patients were grouped into mild group(n=41),moderate group(n=45),and severe group(n=34).Meanwhile,those who underwent health examinations at the same time were regarded as control group(n=120).Real-time fluorescence quantitative PCR method was applied to measure the levels of serum LncRNA BLACAT1 and LncRNA-H19.The serum levels of LncRNA BLACAT1 and LncRNA-H19 were compared between KOA group and the control group.The serum levels of LncRNA BLACAT1 and LncRNA-H19 in KOA patients with different degrees of illness were analyzed.Spearman correlation was applied to analyze the relationship between the expression of serum LncRNA BLACAT1,LncRNA-H19 levels and the severity of KOA patients.Receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of serum LncRNA BLACAT1 and LncRNA-H19 levels for KOA.Results The serum levels of LncRNA BLACAT1(1.31±0.31)and LncRNA-H19(1.41±0.37)in KOA patients were higher than those in the healthy control group(0.99±0.24,1.03±0.25),and the differences were statistically significant(t=8.941,9.322,all P＜0.05).The serum levels of LncRNA BLACAT1(1.53±0.36)and LncRNA-H19(1.71±0.44)in the severe group were higher than those in the mild(1.13±0.27,1.18±0.29)and moderate groups(1.32±0.31,1.40±0.38)(q=7.806,4.183;8.709,5.200),and the serum levels of the moderate group were higher than that of the mild group(q=3.983,3.884),and the differences were statistically significant,respectively(all P＜0.05).Spearman correlation analysis results showed that the serum levels of LncRNA BLACAT1 and LncRNA-H19 were positively correlated with the severity of the disease(r=0.552,0.414,all P＜0.05).The results of ROC showed that the AUCs of LncRNA BLACAT1 and LncRNA-H19 alone diagnosed KOA were 0.860 and 0.869,with specificity of 74.2％and 80.8％,and sensitivity of 66.7％and 69.1％,respectively.The AUC of the combined diagnosis of KOA was 0.966,with a sensitivity and a specificity of 90.0％,81.7％,respectively.Conclusion The serum levels of LncRNA BLACAT 1 and LncRNA-H19 in KOA patients were elevated and were positively correlated with the severity of KOA patients.Combined detection of the two could effectively predict the occurrence of KOA.

Lysostaphin, a specific endopeptidase enzyme derived from Staphylococcus aureus, is a bactericidal agent against Staphylococcus and difficult to be drug-resistant. This study established the monoclonal antibody affinity chromatography to obtain lysostaphin of high purity for drug-use standard. The purified Lysostaphin was of > 95% purity and its recovery rate more than 90%. Moreover, the affinity column kept its efficiency of purification invariable after more than 30 times repeat. Also, the dye release assay validated that the purified lysostaphin had significant bactericidal activity. This method was simple and of high efficacy for the lysostaphin purification and showed its potency in commercial production.
Antibodies, Monoclonal ; immunology ; Chromatography, Affinity ; methods ; Lysostaphin ; biosynthesis ; isolation & purification ; Recombinant Proteins ; biosynthesis ; isolation & purification ; Staphylococcus aureus ; enzymology