1.Study on Cytotoxicity of Codeine Phosphate Tablets
Jiehu OUYANG ; Xianxian PENG ; Ruilian LI
China Pharmacist 2014;(10):1763-1764
Objective:To compare the formula of codeine phosphate tablets produced by two enterprises. Methods: IC50 of the tablets and raw material of codeine phosphate, and the excipients from two enterprises was detected respectively. Results:IC50 of the tablets from A enterprise was 0. 169 mg·ml-1 , that from B enterprise was 0. 140 mg·ml-1 , and the difference was statistically sig-nificant(P<0. 05). IC50 of the raw material from the two enterprises showed no notable difference (P>0. 05). Conclusion: The difference in cytotoxicity of codeine phosphate tablets is manly induced by the excipients, and the products from A enterprise are better than those from B enterprise.
2.Intervention of BCG Polysaccharide and Nucleic Acid in Hypersusceptibility in Guinea Pigs
He TANG ; Ruilian LI ; Zishan LI ; Jiehu OUYANG ; Rongrong WANG
China Pharmacist 2015;(3):394-396,397
Objective:To study the effect and underlying mechanism of BCG polysaccharide and nucleic acid ( BCG-PSN) in hy-persusceptibility in guinea pigs to explore the improvement method for the quality control model of BCG-PSN. Methods:The ovalbumin induced hypersusceptibility animal model was established, the effect of BCG-PSN on hypersusceptibility in guinea pigs was observed. According to the guideline for immunity toxicity study on Chinese traditional medicine and natural medicine, the hypersusceptibility tests were carried out. Serum IgE and histamine were determined by ELISA. Results:The guinea pigs in the model group and the low dosage BCG-PSN group showed strong anaphylactic symptoms, while the middle and high dosage BCG-PNS groups showed fewer symp-toms. The level of IgE in the model group was (1. 673 0 ± 0. 158 6) μg·ml-1 and (1. 683 1 ± 0. 228 1)μg·ml-1 before and after the attacking, respectively, which was higher than that in the control group(P<0. 01). The levels of IgE in the middle and high dos-age BCG-PNS groups were decreased compared with those in the model group before and after the attacking(P<0. 01). The same re-sults were observed in the levels of histamine. Before and after the attacking, the levels of histamine in the model group was (1. 499 7 ± 0. 133 1) ng·ml-1 and (1. 512 1 ± 0. 050 6) ng·ml-1 , respectively, while the levels of histamine in low, middle and high dos-age BCG-PNS groups were decreased compared with those in the model group before and after the attacking(P<0. 01). Conclusion:BCG-PSN can dose-dependently inhibit the anaphylactic reaction induced by ovalbumin.