Objective It describes procedures for designing an analyzing a method comparison experiment for serum albumin(ALB) assay and determining the relative bias between two methods using split patient samples.Method According to the procedure described by the NCCL approved guideline: method comparison and bias estimation using patient samples; 40 patient samples were analyzed in 5 operating days, analyze each patient sample in duplicate using both BCG and BCP method. The duplicates were assessed for each method within the same run. The coefficient of correlation was calculated as well as the bia between the methods.Results In the patient′s serum sample, the bias between BCG and BCP methods were 1.8% at 50g/L or 5.5% at 40 g/L or 11.6% at 30 g/L for albumin. In the commercial quality control serum products, the bias between the two methods varied up to 36%.Conclusion In determination of the patient′s ALB, the bias between BCG and BCP goes up when the concentration of ALB is decreased. But the bias between the two methods varies up to 36% when the quality control serum is determined.

Objective To investigate the application and needs' survey of mobile App health software in the patients with respiratory chronic disease. To make mobile App health software development more targeted and practical in the near future. To provide the foundation for continuous nursing service. Methods A survey research method was adopted in which self-designed questionnaires were administered to 163 patients with respiratory chronic disease from respiratory ward and outpatient clinic. Results Totally 119(73.0%) patients used smartphone, the mean score of experience in using mobile phones was (32.4 ± 14.1), which was in the medium range. There were 80 patients had ever installed and used this kind of App. The frequency of use of mobile App health software was Breathing Exercises App, Healthy Exercise App, Healthy Diet App and Medicine Remind App. The major reason for using of these App was disease prevention, self health care, disease and health monitoring and rehabilitation exercises. There were 120 patients would like to install App health software specific to respiratory chronic disease. They hope the characters of these App were simple operated, practical and without product placement. These App health software should include following function:timely feedback and guidance, providing practical method and help to register. Conclusion Most patients with respiratory chronic disease had the hardware basis and experience basis of using mobile App health software. More targeted and practical mobile App health software for the patients with respiratory chronic diseases should be developed to provide the foundation for continuous nursing service and patient′s self-management.

Objective To construct home-based rehabilitation nursing program for elderly patients with chronic obstructive pulmonary disease based on functional physical fitness index.Methods Through literature search,case review and the daily needs,disease characteristics,physical fitness and other core indicators of elderly patients with chronic obstructive pulmonary disease,the first draft of home rehabilitation nursing program for elderly patients with chronic obstructive pulmonary disease was con-structed.The expert consultation questionnaire was prepared,and 12 experts related to respiratory and rehabilitation were selected through group discussion,and two rounds of expert consultation were conducted.Results The recovery rate of the two rounds of expert consultation questionnaires was 83.3％for both rounds,with an expert authority coefficient of 0.90.The home-based rehabilitation nursing program consists of four primary indicators,eleven secondary indicators,and thirty-three terti-ary indicators.Conclusion The home-based rehabilitation nursing program for elderly patients with chronic obstructive pulmonary disease constructed based on functional physical fitness is feasible,sci-entific and innovative,which can provide theoretical basis for clinical application.

Objective To construct home-based rehabilitation nursing program for elderly patients with chronic obstructive pulmonary disease based on functional physical fitness index.Methods Through literature search,case review and the daily needs,disease characteristics,physical fitness and other core indicators of elderly patients with chronic obstructive pulmonary disease,the first draft of home rehabilitation nursing program for elderly patients with chronic obstructive pulmonary disease was con-structed.The expert consultation questionnaire was prepared,and 12 experts related to respiratory and rehabilitation were selected through group discussion,and two rounds of expert consultation were conducted.Results The recovery rate of the two rounds of expert consultation questionnaires was 83.3％for both rounds,with an expert authority coefficient of 0.90.The home-based rehabilitation nursing program consists of four primary indicators,eleven secondary indicators,and thirty-three terti-ary indicators.Conclusion The home-based rehabilitation nursing program for elderly patients with chronic obstructive pulmonary disease constructed based on functional physical fitness is feasible,sci-entific and innovative,which can provide theoretical basis for clinical application.

Objective? To understand the knowledge of influenza among inpatients before health education and intervention using the sensitive indicators in nursing outcomes. Methods? Using convenience sampling method, 400 inpatients from November 2018 to February 2019 in China-Japan Friendship Hospital were selected as the research objects. Referring to the two outcomes indicators of "knowledge: disease process" and"knowledge: control of infection" in the Classification System of Nursing Outcomes, a Knowledge Questionnaire on Influenza Susceptibility Indicators of Inpatients was developed to investigate and analyze influenza sensitive indicators. A total of 392 valid questionnaires were collected. Results? The 392 patients' scoring rate of Knowledge Questionnaire on Influenza Susceptibility Indicators of Inpatients was 48.1%. There was no significant difference in Knowledge Questionnaire on Influenza Susceptibility Indicators of Inpatients scores among patients of different ages, gender, educational background, living environment and occupation (P＞0.05), all of them had low scores. In the single analysis, there were statistically significant differences in the scores of "influenza symptoms and signs" among patients of different ages, gender, educational background, living environment and occupations (P＜0.05). There were significant differences in the knowledge of "influenza transmission mode" among patients living in different environments (P＜ 0.05). Compared with the two dimensions of "disease process" and "infection control", the score of "disease process" was higher than that of "infection control", and the difference was statistically significant (P＜0.01). Conclusions The situation of understanding sensitive indicators of flu in the inpatients is not very well, the evaluation based on nursing outcome sensitive indicators can help medical staff to understand the inpatients' real status on flu-related knowledge, self-protection awareness, and behaviour and to provide reference of the following health education and prevention management.

OBJECTIVETo establish the method of promoting human peripheral blood mononuclear cell proliferation by polysaccharide of snakegourd root and identify the effects of polysaccharide of snakegourd root on lymphocyte proliferation, T lymphocyte subsets and the different levels of TNF-alpha and IL-6.

METHODThe polysaccharide of snakegourd root preparations were purified with dialysis and ethanol precipitation. The healthy human PBMC were used as the target cells for screening potency of the drugs. MTT colorimetry was established to examine the levels of lymphocyte proliferation on human PBMC by polysaccharide of snakegourd root in vitro. The percents of lymphocyte subsets (CD3+, CD4+, CD8+ T lymphocyte) and the different levels of TNF-a and IL-6 in PBMC were analysed by FCM and ELISA, respectively.

RESULT1.0-50.0 mmol x L(-1) of polysaccharides of snakegourd root showed the significant effects of promoting proliferation of human PBMC (P < 0.05). The percents of CD3+, CD4+, CD8+ T lymphocytes in PBMC treated with 5.0 and 10.0 mmol x L(-1) of polysaccharides of snakegourd root were significantly higher than those of the control group (P < 0.05). The levels of TNF-alpha and IL-6 were significantly higher than those of the control group after 1.0, 5.0, 10.0 mmol x L(-1) of polysaccharides of snakegourd root stimulation on the human PBMC at 8 hours (P < 0.05).

CONCLUSIONThe significant effects on promoting lymphocyte proliferation and activation of the polysaccharide of snakegourd root are confirmed in this study. The percents of lymphocyte subsets are increased in different degrees by the polysaccharide of snakegourd root. The high levels of TNF-alpha and IL-6 are secreted after the polysaccharides of snakegourd root stimulation on the human PBMC, which lays a foundation for further elucidating the immunocompetence effects and mechanism of the polysaccharide of snakegourd root.

Objective To develop a candidate reference method for the measurement of serum glucose based on isotope dilution liquid chromatography tandem mass spectrometry(ID-LC/MS/MS)Methods An internal standard [~(13)C_6]glucose was added to serum samples and equilibrated with endogenous glucose.Serum proteins were removed by a precipitation with anhydrous ethanol.Serum glucose and the internal standard were then reacted with 1-phenyl-3-methyl-5-pyrazolone and the formed derivatives were analyzed by liquid chromatography tandem mass spectrometry with multiple reaction monitoring(MRM).The method was calibrated with bracketing calibrators and serum glucose concentrations were calculated by comparing the peak area ratios of samples with that of the calibrators.Results The within-run,between-run and total coefficients of variation averaged 0.36%,0.47%and 0.61%,respectively.The analytical recoveries ranged from 99.0% to 100.9%.Results of analyzing the certified reference material SRM 965a showed an average biases of-0.20%.Conclusions An ID-LC/MS/MS method for measuring serum glucose has been developed.The method is highly precise and accurate and may be used as a candidate reference method.

Objective To exlore the influence of internal quality control and external quality control assessment(EQA) resulting from applicability of control samples in measurement of whole blood viscosity (WBV) through the analysis and comparison of applicability of 1 non-Newtonian fluid internal quality control sample in 3 viscometers. Methods Viscometer B, C and D were used to measure WBV of 30 blood samples in parallel under the shear rate(SR) of 1 s-1,30 s~(-1) and 200 s~(-1), then the blood SR-WBV curves of 3 viscometers were drawn according to the results. At the same time, viscometers B, C and D were used respectively to determine the WBV of control A 10 times in one day, then the control A SR-WBV curves were mapped. Three viscometers were used to measure the manufactory control samples and control A 5 times in one day for 4 days. Four groups of daily values of manufactory control samples and control A of each instrument were used to carry out F test to calculate whether 4 daily values are difference. Finally, the control A was dispensed in 49 laboratories nationwide chosen for measurement. On the basis of viscometer used, 20 laboratories were classified as group B, 20 laboratories were classified as group C and 9 laboratories were classified as group D. Then the data under SR of 1 s~(-1) were analyzed to calculate the coefficient of variation (CV) in the group. Results There was significant difference among the WBV of blood samples measured by the viscometers B, C and D. The results under SR of 1 s~(-1) declined in turn, and they were highest under SR of 30 s~(-1) followed by the values of viscometer D and B and they were (8.14±0.75), highest under SR of 30 s-1 followed by the values of viscometer B and D, and they were (7.35±0.07), daily values of manufactory control and control A of each instruments in four groups were compared. Under SR of 1 s~(-1), there was no difference between daily values of manufactory control and control A in viscometer B (F = 2.63, 1.37, P > 0.05), and there was no difference of daily values of manufactory control among viscometer C and D (F = 0.33,3. 14, P > 0.05), but significant daily difference existed when control A was tested by viscometer C and D (F = 5.76, 8.00, P < 0.05). Under SR of 30 s~(-1), there was no difference of daily values of manufactory control among 3 viscometers(F =0.31, 0.18, 2.26, P >0.05), and there was no difference of daily values of control A among 3 viscometers' (F = 1.03, 1.83, 2.40, P > 0.05); Under SR of 200 s~(-1), there was no difference of daily values of manufactory control among 3 viscometers (F =2.59, 0.68, 2.96, P > 0.05), and there was no difference of daily values of control A among 3 viscometers (F=2.31, 3.01, 2.28, P>0.05). When control A was tested under SR of 1 s~(-1) in 49 laboratories nationwide, the WBV values in groups of viscometer B, C and D were (18.47±1.30), (11.17±2.38), viscometer D and C were 63.75% and 21.3%. Conclusions Control A could fully mimic the properties of whole blood steadily on viscometer B, but partially mimic viscometer C and D, so the control A is most appropriate for viscometer B. Because current non-Newtonian fluid internal quality control could mimic rheological properties of whole blood under specifically conditions, laboratories should evaluate the consistent degree between control and whole blood, only the candidates which can mimic the properties of whole blood approximately could be chosen as quality control of WBV. When third-party control is chosen to be samples of EQA, its applicability should be in consideration. Pretest should be performed adequately to define applicability of third-party control, so as to reduce the difference among laboratories due to applicability of control and reflect detection quality of laboratories exactly.

Objective To develop a candidate reference method for the measurement of creatinine in human serum based on isotope dilution liquid chromatography tandem mass spectrometry (ID-LC/MS/MS). Methods An isotopically labeled internal standard [<'2>H<,3>] creatinine was added to the serum sample and equilibrated with the endogenous creatinine. The samples were treated with anhydrous ethanol to remove proteins by precipitation. After being washed with chloroform for further clean-up, the samples were analyzed by LC/MS/MS. Serum creatinine was quantified by a bracketing calibration. Results The within-run, between-run and total coefficients of variation ranged from 0.52% to 0.61%, 0.11% to 0.59% and 0.61% to 0.83%, and the averages were 0.57%, 0.43% and 0.73%, respectively. The analytical recoveries ranged from 99.09% to 101.13% with an average of 100.3%.The results of analyzing the certified reference material SRM 909b (Level Ⅰ and Ⅱ) and SRM 967b showed biases of less than 0.4%. Conclusions An ID-LC/MS/MS method for measuring serum creatinine has been developed. The method is highly precise and accurate and may be used as a candidate reference method for serum creatinine measurements.

Objective To develop a candidate reference method for the measurement of urea in human serum based on isotope dilution/gas chromatography/mass spectrometry.Methods [13C,15N2]Urea used as internal standard Was added to the serum sample and equilibrated with endogenous nonlabeled urea.The serum samples were treated with anhydrous ethanol to emove proteins by precipitation.The serum urea and labeled urea were converted into a trimethylsilyl derivative of 2-hydroxypyrimidine and analyzed by gas chromatography/mass spectrometry system with selected ion monitoring.The concentration of serum ureaWas calculated by the theory of bracketing method.Results The average value of within-run oefficient of vailation(CV),between-run CV and total CV of the procedure were 0.38%(ranged from 0.12%to 0.47%),O.62%(ranged form 0.49% to 0.87%)and 0.73%(ranged from 0.51% to 0.93%).Respectively.The analytical recoveries ranged from 99.37% to 100.95%.The resuhs of analyzing the certified refefence material SRM909b(Level Ⅰand Ⅱ)showed a bias less than 0.2%.Conclusion The procedure for measuring urea in serum is a highly accurate and precise method and can be used as a candidate reference method for serum urea assays.