AIM: To explore whether strontium ranelate ( Sr ) promotes osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs) through the Hedgehog/Gli1 signaling pathway.METHODS:BMSCs were isolated from 4-week-old rats by adherent culture and induced to differentiate into osteoblasts .According to the experimental purposes , the cells were exposed to different concentrations of Sr , cyclopamine ( Cy, an inhibitor of Hedgehog receptor ) or Gli1-siR-NA.The expression of Gli1 and Runx2 in the cells was detected by Western blotting .The activity of alkaline phosphatase ( ALP) was measured by the method of colorimetry , and the mineralized nodules were observed under microscope with aliz-arin red staining .RESULTS:Exposure to Sr at concentrations of 0.1 to 5 mmol/L for 7 d markedly increased the expres-sion of Gli1 in the BMSCs , and the increase in Gli1 expression was the most obvious following Sr exposure at concentration of 3 mmol/L.Cy at concentration of 10 μmol/L inhibited Sr-induced up-regulation of Gli1 expression.Transfection of the BMSCs with Gli1-siRNA not only obviously inhibited Sr-induced up-regulation of Gli1 and Runx2 ( a downstream protein of Gli1) expression, but also antagonized Sr-induced enhancement of ALP activity and the formation of mineralized nodules . CONCLUSION:The Hedgehog/Gli1 pathway is involved in Sr-induced osteogenic differentiation of rat BMSCs .

Objective To explore the role of PDSA quality management tool in promoting the construction of hospital medical record quality control audit system,and the path to continuously improve the quality of intelligent audit of medical record first page data.Methods Analyzing the current state of medical record quality management using root cause verification meth-ods,we followed the PDSA quality management theory and process,implementing planning,execution,evaluation,and handling procedures.Special pilot programs,effectiveness validation,and iterative improvement measures were undertaken to enhance the entire process of intelligent medical record quality control review.Results After implementing PDSA cycle management meas-ures,the completion rates for admission records,surgical records,discharge records,and medical record cover pages within 24 hours all reached the preset target values.The accuracy of the main diagnosis selection by physicians significantly improved,re-sulting in a standardized and visualized work mechanism for intelligent medical record quality control.Conclusion The applica-tion of PDSA cycle management can effectively improve and enhance the quality of intelligent review of medical record cover pa-ges,facilitating the construction of a scientific system for intelligent medical record quality control review.