Objeetive To discuss a reasonable postoperative management of breast augmentation by polyacrylamide hydrogel (PAHG) injection.Methods The retrospective study was used to analyze 157 cases which received breast augmentation by PAHG injection.MRI was used in all of cases preoperatively.Among these patients,23 were located by three-dimensional (3D) reconstruction,the content of mono acrylamide in the serum of 71 cases were examined in the hydrogel of 23 cases and in the tissue around the hydrogel of 12 cases,respectively.The silicone gel implants were planted after the removal of PAHG in 7 cases.Results MRI-3D could show the injectants location,scope,layer and integrality more intuitional and more detailed.The momo acrylamide was found in the serum with 7 cases,in the hydrogel with 5 cases,in the tissue around the hydrogel with 3 cases.131 cases gained satisfactory results.All the cases received 3 to 6 months follow-up.The scleroma was not found after the palpation of breast.In the 7 cases that were planted with the silicone gel implants immediately,the shape and texture of the breast were both great.Conclusions The preoperative MRI examination is a first-choice,and if possible,3D reconstruction is better.With the detecting methods so far,there is no strong evidence to support the possibility of PAHG resolving into poisonous acrylamide inside the human body.During the operation,the injectant,the integument around the hydrogel and degenerative tissue should be orthoptically cleared-up as much as possible.Sucking the injectant blindly is not commended.The silicon gel prosthesis is planted to reconstruct the shape of breast immediately,but the prerequisite is that the patients have this demand and that the muscle is intact without inflammation.

Objective: To study the effects of different purification method on the ratio of each component in the suspension of fat granules and the cell viability. Methods: Fat granule suspension was harvested under conventional swelling anesthesia, and divided into staticseparation group, centrifugation group, washing and filtration group.①staticseparation group: based on different gravity separation time (30 s, 1 min, 5 min, 10 min, 30 min, 1 h, 4 h, 12 h, 24 h), the group was subdivided into 9 groups.②Centrifugation group: based on different centrifugation times (3, 5, 10 min) combined withdifferent centrifugation rates (1 000, 2 000, 3 000, 4 000 r/min), this group was subdivided into 12 groups.③Washing and filtration group: According to the layers of gauze (1 layer, 2 layers, 5 layers), this group was subdivided into three groups. Adipocyte activity was measured by Calcein-AM / Hoechst 33342 dual fluorescence staining, and the viable cell rate was calculated. T test was introducedto compare between two samples. One-way AVOVA analysis was used to compare between two groups of samples. Results: Staticseparation group: After gravity separation, the fat granules were separated intooil layer, fat granules layer and liquid layer, and stabilized at 5 min after staticseparation. ②Centrifugation group: The viable cell ratewas highest at 3 min in 2 000 r/min group.③Washing and filtration group: More volume of fat particles were collected by filtration with 2-layer gauze, and the viable cell rate was not significantly reduced.Compared with static 5 min method (86.75±6.29)%, 2 000 r/min 3 min (89.05±7.16)% and double gauze filtration rinse, the cell viability rate was (87.85±5.92)%, and there was no significant difference between the three groups (P> 0.05). Conclusions 5 min static separation, 2-layer gauze filter and 2 000 r/min 3 min fat cell survival rate is the highest among each group, and no significant difference in viable cell rate between these three method . Clinically, 2 000 r/min 3 min is suitable for a small amount of fat filling, washing and filtration is suitable for massive fat filling.

Objective: To measure and analyze the bone volume and thickness of mandibular angle area by computer-assisted CT. Methods: From January 2010 to January 2016, 94 female patients with mandibular angle hypertrophy and bilateral mandibular angle osteotomy were selected. Twenty female patients with non-mandibular surgery were selected as control group (patient examined after nasal augmentation surgery), aged 18 to 37 years, with an average of 27.5 years. Three dimensional CT data of maxillofacial bone of patients were collected. The three dimensional reconstruction and measurement software Mimics 16.0 was used. A three-dimensional model of mandibular angle region was established. The thickness and volume of mandibular angle were measured and analyzed. The total difference between hypertrophic group and normal group was compared by two independent t-test. Results: The thickness of mandibular angle area and mandibular angle area in hypertrophic group were (9.39±1.48) mm and (3 065.25±720.1) mm³ respectively, and the thickness of mandibular angle area and mandibular angle region in control group were (7.48±0.92) mm and (1 276.57±265) mm^3, respectively. There was a significant difference between the two groups (P < 0.05). Conclusions Based on CT scanning data, a method of measuring the bone volume and thickness of mandibular angle region is established, which is helpful to the three-dimensional measurement and analysis of mandibular shape in patients with mandibular angle hypertrophy.

Objective: To investigate the surgical treatment of orbital fractures and ocular traction deformities, and summarize the surgical results. Methods: A retrospective study was conducted on 42 cases of monocular patients with orbital fractures and ocular fractures treated by Medpor Titan and Medpor. Preoperative CT scan and three-dimensional reconstruction were performed to evaluate the maxillofacial fractures, orbital wall defects, orbital content, shift incarceration. During surgery, the periorbital fracture and defect area were exposed, the periorbital shift bone were reduced, Medpor Titan was used to repair the orbital floor, Medpor to correct eyeball retraction. Follow-up observation of postoperative results and to assess the patient′s satisfaction rate, patient’s eyeballs, visual acuity and motor function. Results: All patients had successful operation, the eye trapping deformity were significantly improved or corrected. Patient satisfaction rate was 92.9% (39/42); wound healingswere good, except for 1 infection (4 years after surgery), there was no rejection. Six months after the surgery, the ocular protrusion was reduced in 88.1% (37/42), the degree of diplopia was significantly improved in 90.5% (19/21) and the eye movement disorder corrected by 87.5% (14/16). Conclusions Medpor Titan combined with Medpor repair orbital wall fractures can achieve good results, with high satisfaction rate. Diplopia and eye movement dysfunction can be significantly improved.

OBJECTIVE： To study the effects of bezafibrate （BEZ） and fenofibrate （FEN） on the proliferation of lung adenocarcinoma PC-9 cells and the expression of c-myc. METHODS： The effects of BEZ and FEN （12.5， 25， 50， 100， 200       μmol/L） on the survival rate of PC-9 cells were detected by CCK8 method. PC-9 cells were divided into administration group and control group. Administration group was given low， medium and high concentration （25， 50， 100 μmol/L） of BEZ and FEN； control group was treated with dimethyl sulfoxide for 48 h. Cell cycle distribution and apoptosis were detected by flow cytometry. qRT-PCR was used to detect mRNA relative expression of c-myc in cells. The protein relative expression of c-myc in cells were detected by Western blot assay. RESULTS： The survival rates of PC-9 cells were （80.76±3.2）％， （74.35±5.06）％， （62.8±1.23）％， （59.03±1.55）％， （39.8±1.01）％ under the action of above concentration of BEZ； and the survival rates of PC-9 cells were （74.46±1.30）％， （61.91±4.77）％， （48.95±2.8）％， （37.05±1.55）％， （32.49±1.36）％ under the action of FEN. Compared with control group， G1 phase cell ratio increased significantly in medium and high concentration groups of BEZ and FEN； the apoptotic rate of PC-9 cells was increased significantly in low， medium and high concentration groups of BEZ and FEN； mRNA and protein relative expression of c-myc were decreased significantly， with statistical significance （P＜0.05）. CONCLUSIONS： BEZ and FEN can inhibit the proliferation of PC-9 cells， and down-regulate c-myc expression.